An investigation into the role of lipid peroxidation in the mode of action of aromatic hydrocarbon and dicarboximide fungicides

Orth, Ann B.; Sfarra, A.; Pell, Eva J.

doi:10.1016/0048-3575(92)90106-a

Cited by 24 publications

(8 citation statements)

References 26 publications

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“…However, others have not found this effect (e.g. Orth et al ., 1992, 1993; Cabral & Cabral, 2000). Nevertheless, there is a good correlation between the content of intracellular lipid peroxides and the fungicide concentration when B. cinerea and Mucor mucedo are treated with dicarboximides (Edlich & Lyr, 1992) and, thus, it has been proposed that lipid peroxides (especially those originating from the mitochondrial inner membrane) are involved in the mode of action of dicarboximides (Edlich & Lyr, 1995; Choi et al ., 1997).…”

Section: Introductionmentioning

confidence: 92%

Lipid composition of Botrytis cinerea and inhibition of its radiolabelling by the fungicide iprodione

et al. 2003

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Summary• Botrytis cinerea is an important plant pathogen that causes grey mould in over 200 hosts. It is often controlled by dicarboximides, which have various proposed mechanisms of action, including effects on lipids. Here we have examined the effect of one dicarboximide, iprodione, on lipid metabolism.• B. cinerea , cultured in malt extract media, was challenged with iprodione and its lipids extracted, separated by TLC, and analysed by GLC. Lipid metabolism was followed using [1-14 C]acetate.• Triacylglycerol was the major nonpolar and phosphatidylcholine the main polar lipid in B. cinerea . Linoleate, followed by α -linolenate, were the major fatty acids and most lipid classes had compositions broadly similar to the total fatty acid pattern. Iprodione, at concentrations causing a cessation of growth (5 µM) caused a decrease in polar lipid but not total nonpolar lipid labelling. Within the nonpolar lipids, DAG was better labelled.• The data show that iprodione had a selective effect on lipid metabolism. The altered pattern of labelling suggested that choline (ethanolamine) phosphotransferase would be worth investigating as a primary site of action.

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Section: Introductionmentioning

confidence: 92%

Lipid composition of Botrytis cinerea and inhibition of its radiolabelling by the fungicide iprodione

et al. 2003

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“…Widespread resistance to dicarboximide fungicides, which include iprodione, vinclozolin, and procymidone, was found in B. cinerea within a few years of their introduction (Beever 1983;Leroux et al 1983;Pommer and Lorenz 1995). In spite of many years of investigation, the mode of action of dicarboximides and the resistance mechanisms toward them remained unclear (Orth et al 1992(Orth et al , 1995Edlich and Lyr 1995;Lee et al 2001;Ramesh et al 2001;Vermeulen et al 2001). However, recent data suggest that dicarboximides interfere with the osmotic signal transduction pathway.…”

Section: Introductionmentioning

confidence: 99%

Survey of mutations of a histidine kinase gene BcOS1 in dicarboximide-resistant field isolates of Botrytis cinerea

et al. 2006

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Previously, we cloned a putative osmosensing histidine kinase gene (BcOS1) and revealed that a single amino acid substitution, isoleucine to serine at codon 365, conferred dicarboximide resistance in field isolates of Botrytis cinerea. This point mutation (type I) occurred within the restriction enzyme TaqI site of the wild-type BcOS1 gene. Thus, a procedure was developed for detecting the type I mutation of the BcOS1 gene using a polymerase chain reaction (PCR) in combination with restriction fragment-length polymorphism (RFLP). Diagnosis by PCR-RFLP was conducted on the 105 isolates isolated from 26 fields in Japan. All dicarboximide-sensitive isolates (49 isolates) had the wild-type BcOS1 gene, and the 43 isolates with the type I mutation were resistant to dicarboximides without exception. These data indicate that dicarboximide-resistant isolates with type I mutation are widespread throughout Japan. However, other types of dicarboximide resistance were detected among isolates from Osaka; among the 24 resistant isolates from Osaka, 12 had the BcOS1 gene without the type I mutation. BcOS1 gene sequencing of these resistant isolates classified them into two groups, type II and type III. The type II isolates have three amino acid substitutions within BcOS1p ( 368 Val to Phe, 369 Gln to His, and 447 Thr to Ser). The type III isolates have two amino acid substitutions within BcOS1p ( 369 Gln to Pro and 373 Asn to Ser). These amino acid changes are located on the amino acid repeat domain in BcOS1p. The three types of resistant isolates were all moderately resistant to dicarboximides without significant osmotic sensitivity, and their pathogenicity on cucumber leaves was also very similar to that of the wild-type isolate.

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“…By contrast, suggestions have been made that proline might protect plants from heavy metal toxicity (Farago & Mullen, 1979 ;Smirnoff & Cumbes, 1989 ;Kavi Kishor et al, 1995). Another salient point is that diverse kinds of stresses, inter alia, induce lipid peroxidation, which leads to disruption of membrane functions and harmful effects on plant cells (Orth et al, 1991 ;Seel et al, 1992 ;DeLong & Steffen, 1997). There appears to be a relationship between lipid peroxidation and proline accumulation in plants subjected to diverse kinds of stresses.…”

Section: mentioning

confidence: 99%

Heavy‐metal‐induced proline accumulation and its role in ameliorating metal toxicity in Chlorella vulgaris

1999

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Exposure of Chlorella vulgaris to elevated concentrations of copper, chromium, nickel and zinc led to intracellular accumulation of high concentrations of these metals. Concomitantly, accumulation of free proline occurred, depending on the concentration of metals in the external medium or in the cell. The greater the toxicity or accumulation of a metal, the greater the amount of intracellular proline in algal cells. However, higher concentrations of copper and chromium were inhibitory to proline accumulation by the test organism. The accumulation of proline was triggered within a few hours of metal treatment. Test metals also induced lipid peroxidation ; copper was the most efficient inducer whereas zinc was the least. Pretreatment of C. vulgaris with proline counteracted metal-induced lipid peroxidation and potassium ion efflux. Thus the present work shows a protective effect of proline on metal toxicity through inhibition of lipid peroxidation.

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An investigation into the role of lipid peroxidation in the mode of action of aromatic hydrocarbon and dicarboximide fungicides

Cited by 24 publications

References 26 publications

Lipid composition of Botrytis cinerea and inhibition of its radiolabelling by the fungicide iprodione

Lipid composition of Botrytis cinerea and inhibition of its radiolabelling by the fungicide iprodione

Survey of mutations of a histidine kinase gene BcOS1 in dicarboximide-resistant field isolates of Botrytis cinerea

Heavy‐metal‐induced proline accumulation and its role in ameliorating metal toxicity in Chlorella vulgaris

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