An Investigation of Growth Factors and Lactoferrin in Naturally Occurring Ovine Pulmonary Adenomatosis

Sozmen, Mahmut; Beytut, Enver

doi:10.1016/j.jcpa.2012.04.004

Cited by 10 publications

(9 citation statements)

References 104 publications

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“…An immunoreactivity score (IRS) was obtained by multiplying the immunolabelling intensity score (0–3) by the percentage of immunolabelled cells. The IRS ranged from 0 to a maximum value of 300 (Sözmen & Beytut, ; Sözmen, Tunca, & Erginsoy, ).…”

Section: Methodsmentioning

confidence: 99%

Kisspeptin‐10 and the G protein‐coupled receptor 54 are differentially expressed in the canine pregnant uterus and trophoblast cells

Schäfer-Somi

Kaya

et al. 2016

Reprod Domestic Animals

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ContentsUterine tissue was collected from bitches after ovariohysterectomy at different times after ovulation. Samples were assigned to four groups: metestrous non-pregnant, day 10-12, n = 4; pre-implantation, day 10-12, n = 9; post-implantation, day 18-25, n = 13; mid-gestation, day 30-40, n = 7. RT-qPCR detection was performed for kiss1 and the G protein-coupled receptor 54 (GPR54, specific receptor for kisspeptin). In 0-300). In non-pregnant and pre-implantation tissues, gene expression was highly variable for kiss1 and GPR54. Expression of GPR54 was higher before embryo adhesion than during post-implantation and mid-gestation (p < .05), whereas there was no difference found between groups for kiss1. Except during the pre-implantation period, KP-10 expression was higher in the non-pregnant uterus compared to all gestational periods investigated, indicating a pregnancy-related downregulation. In the preimplantation period, KP-10 was present in larger vessels only, whereas the presence of GPR54 in vessels was found in all samples, with most labelling in the postimplantation period. KP-10 was present in superficial uterine glands, GPR54 in superficial and deep uterine glands of the post-implantation uterus. In myocytes, the highest staining for KP-10 was seen in the non-pregnant uterus, whereas the highest staining for GPR54 was seen in post-implantation and mid-gestation. Syncytiotrophoblast cells stained for both KP-10 and GPR54 in post-implantation and mid-gestation, with maximum intensity for GPR54 in the latter. We conclude that KP-10 and GPR54 are expressed in the canine uterus and trophoblast cells. However, during pregnancy, expression of both proteins seems to be differentially regulated.

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Section: Methodsmentioning

confidence: 99%

Kisspeptin‐10 and the G protein‐coupled receptor 54 are differentially expressed in the canine pregnant uterus and trophoblast cells

Schäfer-Somi

Kaya

et al. 2016

Reprod Domestic Animals

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“…On this basis, colostrum proteins can be divided into two major categories: i) high abundance proteins, mainly immunoglobulins and caseins, and, ii) a wide range of low abundant proteins. In this category are included proteins that contribute to protection of newborns against bacterial and viral infections [ 10 , 11 ] and other postpartum environmental challenges such as complement factors, acute-phase proteins, anti-microbial proteins and peptides, and cytokines [ 12 , 13 ], and proteins that contribute to the nutrition and to the development of various parts of newborn organism, such as growth-promoting components, important for development of gastrointestinal tract [ 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Proteomics-Driven Analysis of Ovine Whey Colostrum

et al. 2015

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The aim of this study was to shed light in to the complexity of the ovine colostrum proteome, with a specific focus on the low abundance proteins. The ovine colostrum is characterized by a few dominating proteins, as the immunoglobulins, but it also contains less represented protein species, equally important for the correct development of neonates. Ovine colostrum, collected immediately after lambing, was separated by 1D SDS-PAGE. Proteins bands were digested with trypsin and the resulting peptides were analyzed by LC-MS/MS. On the basis of the Swiss-Prot database, a total of 343 unique proteins were identified. To our knowledge, this study represents the most comprehensive analysis of ovine colostrum proteome.

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“…In a recent article, El-Ashker et al (2018) suggested that, supplemented with Lactobacillus sp., is a possible nutritional supplements to support the immune system in healthy lambs. Due to its role in the protection against microbial infection or its anti-viral activities, it also has been administrated as prebiotic to prevent colonization and excretion Escherichia Coli or against the ovine pulmonary adenomatosis (Sozmen and Beytut, 2012).…”

Section: Discussionmentioning

confidence: 99%

Identification of possible new salivary biomarkers of stress in sheep using a high-resolution quantitative proteomic technique

Escribano

Horvatić

Contreras-Aguilar

et al. 2019

Research in Veterinary Science

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The aim of this study was to identify biological pathways and proteins differentially expressed in the saliva proteome of sheep after the application of a model of stress, using high-resolution quantitative proteomics. In addition, one of the proteins differently expressed was verified and evaluated as a possible biomarker of stress in this species. Saliva paired samples from eight sheep before and after the application of a model of stress based on shearing were analysed using tandem mass tags (TMT). The TMT analysis allowed for the identification of new stress-related metabolic pathways and revealed 13 proteins differentially expressed between before and after the stress. Six of these proteins pertain to four major metabolic pathways affected, namely: canonical glycolysis, oxygen transport, neural nucleus development, and regulation of actin cytoskeleton reorganization. The rest of proteins were unmapped original proteins such as acyl-coenzyme-A-binding protein; complement C3; alpha-2-macroglobulin isoform-X1; type-II small proline-rich protein; lactoferrin; secretoglobin family-1D-member; and keratin, type-II cytoskeletal 6. Of these proteins, based on its biological significance and specific immunoassay availability, lactoferrin was selected for further validation. The immunoassay intra-and inter-assay coefficients of variation were lower than 13%. The method showed good linearity under dilution and recovery, and the detection limit was low enough to detect salivary lactoferrin levels. A significant decrease (P < 0.01) in salivary lactoferrin concentration in the sheep following the application of the model of stress was observed, suggesting that this protein could be a potential salivary biomarker of stress situations in sheep.

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An Investigation of Growth Factors and Lactoferrin in Naturally Occurring Ovine Pulmonary Adenomatosis

Cited by 10 publications

References 104 publications

Kisspeptin‐10 and the G protein‐coupled receptor 54 are differentially expressed in the canine pregnant uterus and trophoblast cells

Kisspeptin‐10 and the G protein‐coupled receptor 54 are differentially expressed in the canine pregnant uterus and trophoblast cells

Proteomics-Driven Analysis of Ovine Whey Colostrum

Identification of possible new salivary biomarkers of stress in sheep using a high-resolution quantitative proteomic technique

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