An investigation of low frequency sound waves as a means of inhibiting barnacle settlement

Interactive effects of salinity and adult extract upon settlement of the estuarine barnacle Balanus improvisus (Darwin, 1854)

“…Loss of discriminating settlement behavior in aged cyprids of B. amphitrite occurs in the presence oflow-frequency sound waves (Branscomb & Rittschof, 1984), polystyrene (Rittschof et aI., 1984a) and bacterial films (Maki et aI., 1988); although it was later shown that some bacteria are capable of inhibiting settlement of older cyprid larvae (Maki et aI., 1990).…”

“…These include: relative density and location of larvae in the water column (Grosberg, 1982;Connell, 1985;Gaines et al, 1985;Shanks, 1986); physical attributes, such as light, flow velocity and spatial heterogeneity of potential settlement sites (Crisp, 1955;Rittschofet al, 1984;Wethey, 1984;Chabot & Bourget, 1988;Eckman et al, 1990); cyprid age (Crisp & Meadows, 1963;Branscomb & Rittschof, 1984;Rittschof et al, 1984a;Maki et al, 1988Maki et al, , 1990; and chemical cues, both stimulatory and inhibitory, associated with adult and larval barnacles, symbiotic fauna, flora, microbial flora and potential predators (Knight-Jones, 1953;Crisp & Meadows, 1962, 1963Larman & Gabbott, 1975;Moyse & Hui, 1981;Strathmann et al, 1981;Rittschof et al, 1984aRittschof et al, ,b, 1985Rittschof et al, , 1986aStanding et al, 1984;Rittschof, 1985;Raimondi, 1988;Johnson & Strathmann, 1989;Maki et al, 1988Maki et al, , 1990Crisp, 1990).…”

A settlement inhibition assay with cyprid larvae of the barnacle Balanus amphitrite

“…It is also possible that the settlement behaviour of the cyprid larvae, involving the secretion of a temporary adhesive (footprint) [17], may be intrinsically more susceptible to chemical stressors. Indeed, settlement of barnacle cyprids is a complex phenomenon integrating the behavioural, biochemical and physiological responses of the organism [17,18] Balanus amphitrite is an excellent test organism for ecotoxicological studies that aim at the rapid screening of toxicants because of its extensive distribution range, year-round reproductive cycle, rapid larval development, the ease of obtaining synchronous cultures, and the predictable settlement in static conditions [5,19]. Results of this study strongly suggest that the barnacle settlement inhibition assay can be used to complement the battery of acute tests for ecotoxicological assessment of contaminants in Hong Kong marine waters.…”

Indole Derivatives as Potent Inhibitors of Larval Settlement by the Barnacle,Balanus amphitrite

“…The cyprids were stored in the dark at 5°C until the day of use. 10) Biological assay.8) Each test compound (0.05 mg) dissolved in 0.1 ml of methanol was dried in a polystyrene Petri dish (35 mm in diameter) at 23°C, before 5 ml of seawater filtered through a 0.45 J.lm pore-size filter was added to adjust the sample concentration to 10 ppm. Ten cyprids (day 5-7) of B. amphitrite, at the settling stage, were then inoculated into each Petri dish, triplicate dishes being prepared for each set of experiments.…”