The variation in the refractive index of control glass samples submitted from scenes of crime was investigated. Refractive index data was compiled for four types of flat glass: toughened float glass, non-toughened float glass, non-float glass, and wired glass. From the case data, additional control glass samples for each type of glass were simulated. This was done by standardizing and pooling the data for each type of glass and then drawing a predetermined number of measurements from a given data pool. The mean, estimate of the standard deviation, and 95% and 99% confidence intervals were calculated from these larger sets of simulated, numerical data. The statistical results for each type of glass examined were tabulated and worked examples based on the data are provided. The author suggests that, as a guideline, a minimum of 17 refractive index measurements be made when sampling a toughened float glass control, and a minimum of nine refractive index measurements be made when sampling a non-toughened float glass control.