An Investigation of Removed Cultivated Epithelial Transplants in Patients After Allocultivated Corneal Epithelial Transplantation

Cooper, Leanne J.; Fullwood, Nigel J.; Koizumi, Noriko; Nakamura, Fuminori; Kinoshita, Shigeru

doi:10.1097/00003226-200404000-00004

Cited by 16 publications

(4 citation statements)

References 14 publications

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“…Traditional therapy includes protease inhibitors, antibiotics, regeneration stimulators, and surgical intervention. Despite the availability of methods for the treatment of corneal burns, regeneration of the corneal tissue and successful recovery of vision are a challenge [3].…”

Section: Introductionmentioning

confidence: 99%

Effects of cultured human adipose‐derived stem cells transplantation on rabbit cornea regeneration after alkaline chemical burn

Lin¹,

Lai²,

Tai³

et al. 2012

The Kaohsiung J of Med Scie

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Ocular chemical burn is a severe injury with poor outcomes. Immediate and appropriate management is highly related to prognosis. We studied the effect of cultured human adipose tissue-derived stem cells on the regeneration of the rabbit cornea after alkaline chemical burn, using used human adipose tissue-derived stem cells as the source material. Immediately after the chemical burn, the experimental eye received a single subconjunctival injection of a stem cell suspension (1.3 × 10(5) cells/0.2 mL), with the other eye serving as control. Rabbits were sacrificed and specimens taken 30 days after injection. The experimental group showed faster wound healing than the control group, and the result for the experimental group was clearer cornea medium. Histologically, there were five to six epithelial cell layers on the corneas of the experimental group as compared to two to three cell layers on the corneas of the control group. Wilcoxon signed rank test showed a significant difference in the epithelial cell layers between the two groups. Surface markers for connexin 43 (Cx43), β-catenin, E-cadherin, and P63 were analyzed. Cx43 and β-catenin showed significant change, as determined by the Wilcoxon signed rank test, which indicated good cell renewal during repair of the corneal epithelium damaged by the chemical burn. E-cadherin and P63 showed no significant change during the epithelium healing process. Transplantation of cultured human adipose tissue-derived stem cells as a treatment for a corneal chemical burn promotes cell renewal and assists in damage repair.

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Section: Introductionmentioning

confidence: 99%

Effects of cultured human adipose‐derived stem cells transplantation on rabbit cornea regeneration after alkaline chemical burn

Lin¹,

Lai²,

Tai³

et al. 2012

The Kaohsiung J of Med Scie

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“…This method, however, is only successful in unilateral limbal stem cell deficiency. Homologous ex vivo expanded limbal stem cells are not superior to homologous limbal grafts (Cooper et al. 2002).…”

Section: Introductionmentioning

confidence: 99%

Corneal surface reconstruction using adult mesenchymal stem cells in experimental limbal stem cell deficiency in rabbits

Reinshagen¹,

Auw-Haedrich²,

Sorg

et al. 2009

Acta Ophthalmologica

106

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ABSTRACT.Purpose: To investigate the ability of mesenchymal stem cells (MSC) to transdifferentiate to corneal epithelial cells in experimental limbal stem cell deficiency in rabbits. Methods: Total limbal stem cell deficiency was produced in 21 right eyes of 21 New Zealand rabbits; 6 eyes served as controls (group 1, G1). After removal of the conjunctival overgrowth, five eyes received amniotic membrane transplantation (AMT; G2). In four eyes, autologous limbal stem cell transplantation from the healthy eye was performed with AMT (G3). In another six eyes, enriched autologous MSC were injected under the amniotic membrane (AM) (G4). Within 280 days, corneoscleral discs were analysed for goblet cells, cytokeratin (CK) 3 ⁄ 12, connexin 43, b 1 -integrin, CK 19, a-enolase, p63 and ATP-binding cassette transporter subtype G-2 (ABCG-2) distribution patterns. Results: Cultivated MSC were positive for CK 3 ⁄ 12 and a-enolase, but negative for ABCG-2, p63 and connexin 43. On rabbit corneas, CK 3 ⁄ 12 was expressed in all corneal regions in all groups, but with significantly different intensities. Among all other parameters, expression levels of ABCG-2, b 1 -integrin and connexin 43 were significantly different between the transplanted groups and the control group. After a mean follow-up time of 172 (47-280) days, goblet cells were rarely present in the central cornea (G1-4). Conclusion: CK 3 ⁄ 12 is not highly specific for differentiated corneal epithelium. Further, goblet cells are not a reliable marker for conjunctivalization in rabbits. Expression of ABCG-2, b 1 -integrin and connexin 43 after mesenchymal stem cell transplantation may indicate their ability to maintain their stem cell character or to transdifferentiate to epithelial progenitor cells.

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“…The drawback is that little is known about the integration of the amniotic membranes after grafting. Indeed, Cooper et al 30 have reported that the amniotic membrane remains intact for 1 year after cultivated corneal epithelial transplantation using an amniotic membrane carrier.…”

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confidence: 99%

Ocular Surface Reconstruction Using Autologous Rabbit Oral Mucosal Epithelial Sheets Fabricated Ex Vivo on a Temperature-Responsive Culture Surface

Hayashida

Nishida

Yamato

et al. 2005

Invest. Ophthalmol. Vis. Sci.

122

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An Investigation of Removed Cultivated Epithelial Transplants in Patients After Allocultivated Corneal Epithelial Transplantation

Abstract: We suggest that the process of allograft rejection is responsible for the corneal epithelial loss and that this is followed by conjunctival invasion onto the amniotic membrane.

Cited by 16 publications

References 14 publications

Effects of cultured human adipose‐derived stem cells transplantation on rabbit cornea regeneration after alkaline chemical burn

Effects of cultured human adipose‐derived stem cells transplantation on rabbit cornea regeneration after alkaline chemical burn

Corneal surface reconstruction using adult mesenchymal stem cells in experimental limbal stem cell deficiency in rabbits

Ocular Surface Reconstruction Using Autologous Rabbit Oral Mucosal Epithelial Sheets Fabricated Ex Vivo on a Temperature-Responsive Culture Surface

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