An investigation of the genotoxic effects of N -nitrosomorpholine in mammalian cells

Hazard assessment of nitrosamine and nitramine by-products of amine-based CCS: Alternative approaches

“…However, although NPIPz is positive when tested in the presence of metabolic activation in an Ames test with Salmonella typhimurium strain 1535 (Zeiger and Sheldon, 1978), this positive result is canceled by the negative result obtained with the in vitro HPRT gene mutation test with Chinese Hamster Ovary cells in the presence of metabolic activation (Jones et al, 1981). In a comparable test with Chinese Hamster Lung cells, the CCS nitrosamines NMOR (Robichová et al, 2004) and NDELA (Liu and Russell, 2008) did test positive. Still NPIPz is carcinogenic (see Table 3), and it might be a (very) weak mutagen.…”

N–nitrosamines are associated with shorter telomere length

“…N-nitrosamines have been reported to induce oxidative stress and DNA damage (43)(44)(45)(46), and the main mechanism suggested for N-nitrosamine carcinogenesis is through the formation of DNA adducts that can cause mutations (47,48). However, most data are derived from tobacco-specific nitrosamines.…”

Cytotoxicity, mutagenicity, cellular uptake, DNA and glutathione interactions of lipophilic trans-platinum complexes tethered to 1-adamantylamine

“…The results were statistically evaluated by Student t-test. Other details can be found in the paper published previously [17].…”

“…Mutation frequency was detected by counting 6-TG resistant colonies in V79 cells in the same way as described previously [17]. Briefly, these cells were seeded (3.5 Â 10 5 , in triplicate per sample) in petri dishes (100 mm) and incubated at 37°C in a 5% CO 2 atmosphere.…”