An Investigation of the Relationship Between the Enos Gene Polymorphism and Diagnosed Migraine

Abstract

We investigated the phenotype-genotype association of the following endothelial nitric oxide synthase (eNOS) gene polymorphisms, rs743506, rs2070744, rs1799983, rs180079, rs3918226, rs207468799 and rs148554851, in patients suffering from migraine living in Edirne, Turkey. A total of 175 individuals, who had been diagnosed with migraine between April 2013 and December 2013, at the Neurology Department, Trakya University Medical Faculty, Edirne, Turkey, and 125 healthy controls were recruited. The above gene polymorphisms were analyzed from genomic DNA in both patient and control groups, using the pyro-sequencing method.The eNOS rs1799983 TT genotype frequency in migraine patients who had a headache duration of longer than 24 hours was statistically significantly higher than in patients who had migraine attacks that lasted under 24 hours (p = 0.047). In terms of the AGGTGGA haplotype, the severity of headache was statistically significant, and was found to be severe in 61.0% (p = 0.0001). Also in terms of the AGGTGGA haplotype, the duration of headache was statistically significant, and was >24 hours in 56.0% of patients (p = 0.008).In our study, there was no significant genotypephenotype relationship between eNOS rs743506, rs2070744, rs1799983, rs180079, rs3918226, rs207468799 and rs148554851 gene polymorphisms and migraine patients with and without aura living in Edirne, Turkey. The AGGTGGA haplotype constitutes a risk in terms of the severity and the duration of headaches in patients with migraine. This risk is significantly higher in patients with migraine with aura than patients with migraine without aura.

Endothelial nitric oxide synthase (−786T>C) polymorphism and migraine susceptibility

“…The characteristics of the included studies, which were published between 2005 and 2017, were presented in Table 1 . Two studies were from Brazil, [ 14 , 19 ] 2 from Turkey, [ 1 , 5 ] 1 from the United Kingdom, [ 18 ] and the last one was from Iran. [ 20 ] A total of 763 patients with migraine and 560 healthy controls were included in this meta-analysis.…”

“…For migraine subtypes, 4 studies were included. [ 1 , 5 , 14 , 19 ] We tried to contact the authors of the remaining 2 studies for details. However, no reply was received.…”

“…An article was relevant if it reported original data from case–control study, regardless of language, investigating the correlations between eNOS polymorphism (−786T>C) and migraine susceptibility. Controls were healthy individuals without migraine randomly selected from general populations after excluding those with underlying diseases (e.g., cardiovascular, renal, hepatic, gastrointestinal, pulmonary, endocrine, auto-immune, psychiatric diseases), [ 1 , 5 , 14 , 19 ] or whose health was established by medical diagnosis. [ 18 , 20 ] Studies were excluded if one of the following existed: studies with insufficient genotyping data of patients or control group; case reports; review articles; experiment researches; case-only studies.…”

“…Migraine is a chronic paroxysmal neurological disorder characterized by attacks of moderate or severe headache and reversible neurological and systemic symptoms that affects approximately 12% to 16% of the population. [ 1 , 2 ] Migraine often cause significant disability and impaired quality of life, adversely affecting the activities of daily living and work-related productivity in many patients. [ 3 ] Migraine is clinically diagnosed according to the criteria set out by the International Headache Society (IHS).…”

Migraine in the era of precision medicine

“…However, the pathogenesis of migraine is not clear enough. Therefore, recent studies have devoted the genetic susceptibility research which may also be a neurobiological factor in the etiology of this disorder (12)(13)(14). Familial hemiplegic migraine (FHM), an autosomal dominant migraine with special aura, has been identified mutations in three causal genes (15).…”