An Investigation of the Sex Difference in the Diuretic Response to Ethacrynic Acid in Mice

Cooling, M.J.; Sim, M.F.

doi:10.1111/j.1476-5381.1977.tb07536.x

Cited by 4 publications

(2 citation statements)

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“…), theophylline (as theophylline 2 /ethylenediamine, 45 mg/ kg), or vehicle (0.9 ml/30 g b.wt. of 0.85% NaCl) given by oral gavage as described (Sim and Hopcroft, 1976;Cooling and Sim, 1977). The mice were then placed in metabolic cages (Tecniplast, Hohenpeissenberg, Germany) for quantitative urine collections over 3 h without access to food or water as described (Yao et al, 2004).…”

Section: Experimental Protocolmentioning

confidence: 99%

Requirement of Intact Adenosine A₁Receptors for the Diuretic and Natriuretic Action of the Methylxanthines Theophylline and Caffeine

Rieg¹,

Steigele²,

Schnermann³

et al. 2004

J Pharmacol Exp Ther

113

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Although the diuretic and natriuretic effects of the methylxanthines caffeine and theophylline are well established, the mechanisms responsible for these effects are unclear and may be related to inhibition of phosphodiesterases and/or antagonism of adenosine receptors. With regard to the latter, pharmacological blockade of A 1 receptors can induce diuresis and natriuresis by inhibition of proximal tubular reabsorption. To elucidate the role of the A 1 receptor in renal actions of methylxanthines, experiments were performed in A 1 receptor knockout (A 1 RϪ/Ϫ) and littermate wild-type (A 1 Rϩ/ϩ) mice. Urinary excretion was determined in awake mice in metabolic cages over 3 h in response to theophylline (as theophylline 2 / ethylenediamine, 45 mg/kg), caffeine (45 mg/kg), or vehicle (0.9 ml/30 g b.wt. of 0.85% NaCl) given by oral gavage. Theophylline and caffeine elicited a diuresis and natriuresis (in absolute terms and related to urinary creatinine excretion) in A 1 Rϩ/ϩ but not in A 1 RϪ/Ϫ mice. In a second series, the renal effect of intravenous application of theophylline (30 mg/kg) was determined in clearance experiments under anesthesia. This study revealed that the blunted diuretic and natriuretic effect of theophylline in A 1 RϪ/Ϫ mice was not due to different responses in blood pressure or glomerular filtration rate. The data indicate that an intact A 1 receptor is necessary for caffeine-and theophylline-induced inhibition of renal reabsorption causing diuresis and natriuresis. This is consistent with the assumption that A 1 receptor blockade mediates these effects.Caffeine and theophylline are members of the methylxanthine family that are very widely consumed and exert well described behavioral effects. In the kidney, methylxanthines induce diuresis and natriuresis, an effect first described by Davis and Shock (1949). The mechanisms by which methylxanthines act as diuretics and natriuretics are not completely understood. Since methylxanthines are nonselective adenosine receptor antagonists (Fredholm et al., 2001), it is conceivable that the changes in urine excretion are the result of inhibition of the renal actions of endogenous adenosine.

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Section: Experimental Protocolmentioning

confidence: 99%

Requirement of Intact Adenosine A₁Receptors for the Diuretic and Natriuretic Action of the Methylxanthines Theophylline and Caffeine

Rieg¹,

Steigele²,

Schnermann³

et al. 2004

J Pharmacol Exp Ther

113

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“…This could be attributed to a hormone-dependent difference in plasma levels (27). A sex-specific diuresis has also been observed in mice with ethacrynic acid (11).…”

Section: Diuretic Effectmentioning

confidence: 90%

Muzolimine

Garthoff

Hanisch

1984

Cardiovascular Drug Reviews

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Effect of ethacrynic acid on the anticonvulsant activity of the second-generation antiepileptics against maximal electroshock-induced seizures in mice

2009

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An Investigation of the Sex Difference in the Diuretic Response to Ethacrynic Acid in Mice

Cited by 4 publications

References 9 publications

Requirement of Intact Adenosine A₁Receptors for the Diuretic and Natriuretic Action of the Methylxanthines Theophylline and Caffeine

Requirement of Intact Adenosine A₁Receptors for the Diuretic and Natriuretic Action of the Methylxanthines Theophylline and Caffeine

Muzolimine

Effect of ethacrynic acid on the anticonvulsant activity of the second-generation antiepileptics against maximal electroshock-induced seizures in mice

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An Investigation of the Sex Difference in the Diuretic Response to Ethacrynic Acid in Mice

Cited by 4 publications

References 9 publications

Requirement of Intact Adenosine A1Receptors for the Diuretic and Natriuretic Action of the Methylxanthines Theophylline and Caffeine

Requirement of Intact Adenosine A1Receptors for the Diuretic and Natriuretic Action of the Methylxanthines Theophylline and Caffeine

Muzolimine

Effect of ethacrynic acid on the anticonvulsant activity of the second-generation antiepileptics against maximal electroshock-induced seizures in mice

Contact Info

Product

Resources

About

Requirement of Intact Adenosine A₁Receptors for the Diuretic and Natriuretic Action of the Methylxanthines Theophylline and Caffeine

Requirement of Intact Adenosine A₁Receptors for the Diuretic and Natriuretic Action of the Methylxanthines Theophylline and Caffeine