An Investigation of Wound Healing in Sugar Beet Roots Using Light and Fluorescence Microscopy

Abstract

Following impact, wound healing was investigated in roots of sugar beet using¯uorescence microscopy in conjunction with a conventional lignin test. Samples of sugar beet roots were harvested at dierent stages of development and impacted in the laboratory with a falling bolt delivering 1±4 Joules. A response in the form of deposition of brown material, presumed to be melanin, along the outer and inner walls of cells at the wound surface was observed within 3 d of impact. This material eventually became granular in appearance. Formation of a ligno-suberized boundary layer from cells present at the time of impact ®rst occurred in 16-week-old roots 9 d after impact. Intensity of calco¯uor uorescence supported the ®ndings made using light microscopy. As wound healing progressed with time, the intensity of calco¯uor¯uorescence declined, demonstrating interference by wound healing products with calco¯uor binding. Aggressive harvest and subsequent storage dramatically reduced calco¯uor¯uorescence indicating that this dye may have potential value in the assessment of tissue damage.# 2001 Annals of Botany Company

Identification of Hybrids in Potamogeton: Incongruence between Plastid and ITS Regions Solved by a Novel Barcoding Marker PHYB

“…Although PHYB -related subgroups show an expansion in Arabidopsis that PHYD and PHYE are closely related to PHYB , there is no evidence for concerted evolution for PHYB [40]. Moreover, PHYB has been used for many phylogenetic studies [28, 43]. Southern blot analysis and PCR supply show that PHYB is a single-copy gene in grasses except maize [28, 44].…”

A Comprehensive Review of Topical Odor-Controlling Treatment Options for Chronic Wounds

“…Sugar is a readily available, cheap, and biocompatible substance that has been used to treat multiple wound types 109–114. Chiwenga and colleagues87 treated 71 patients with malodorous, painful wounds by using sugar dressings.…”

A Comprehensive Review of Topical Odor-Controlling Treatment Options for Chronic Wounds

“…93 Sugar is a readily available, cheap, and biocompatible substance that has been used to treat multiple wound types. [109][110][111][112][113][114] Chiwenga and colleagues 87 treated 71 patients with malodorous, painful wounds by using sugar dressings. Th ey reported a reduction in mean patient odor scores from 5.45 out of 10 at baseline to a mean of 2.94 at 10 days' treatment.…”