An investigation on the cytotoxicity and caspase-mediated apoptotic effect of biologically synthesized silver nanoparticles using Podophyllum hexandrum on human cervical carcinoma cells

Jeyaraj, Murugaraj; Rajesh, Manoharan; Renganathan, Arun; MubarakAli, Davoodbasha; Sathishkumar, Gnanasekar; Sivanandhan, Ganeshan; Dev, Gnanajothi Kapil; Manickavasagam, Markandan; Premkumar, Kumpati; Thajuddin, Nooruddin; Ganapathi, A.

doi:10.1016/j.colsurfb.2012.09.042

Cited by 274 publications

(118 citation statements)

References 46 publications

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“…268 Similarly, nanosilver obtained from S. grandiflora also caused cytotoxicity, oxidative stress, and apoptosis in tumor cells. 269 Moreover, green-synthesized AgNPs from the leaves extract of Podophyllum hexandrum and Suaefa monoica were examined and found to show cytotoxic activity and apoptotic effect, respectively. 270,271 Piao et al demonstrated that OH radicals released by the AgNPs attacked cellular molecules including DNA, proteins, and lipids to induce oxidative damages.…”

Section: Anticancer Property Of Agnpsmentioning

confidence: 99%

Nanosilver: new ageless and versatile biomedical therapeutic scaffold

Khan¹,

Saleh²,

Wahab³

et al. 2018

IJN

168

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Silver nanotechnology has received tremendous attention in recent years, owing to its wide range of applications in various fields and its intrinsic therapeutic properties. In this review, an attempt is made to critically evaluate the chemical, physical, and biological synthesis of silver nanoparticles (AgNPs) as well as their efficacy in the field of theranostics including microbiology and parasitology. Moreover, an outlook is also provided regarding the performance of AgNPs against different biological systems such as bacteria, fungi, viruses, and parasites (leishmanial and malarial parasites) in curing certain fatal human diseases, with a special focus on cancer. The mechanism of action of AgNPs in different biological systems still remains enigmatic. Here, due to limited available literature, we only focused on AgNPs mechanism in biological systems including human (wound healing and apoptosis), bacteria, and viruses which may open new windows for future research to ensure the versatile application of AgNPs in cosmetics, electronics, and medical fields.

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Section: Anticancer Property Of Agnpsmentioning

confidence: 99%

Nanosilver: new ageless and versatile biomedical therapeutic scaffold

Khan¹,

Saleh²,

Wahab³

et al. 2018

IJN

168

View full text Add to dashboard Cite

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“…Also, Green leaf extract of Podophyllum hexandrum -encapsulated AgNPs was prepared and found to cause DNA damage and caspase-mediated cell death of human cervical cancer cell selectively [143]. Moreover, crystalline gold nanoparticles synthesized using Podophyllum hexandrum L. were found to exhibit an effective anticancer activity by inducing oxidative stress, cell cycle arrest, DNA damage, and activation of caspase cascade, leading to mitochondrial dysfunction eventually ensuing in apoptosis of cancer cells [144].…”

Section: Herbal Drug Delivery Systems Against Cervical Cancermentioning

confidence: 99%

Possible role of nanocarriers in drug delivery against cervical cancer

Gupta

2017

Nano Reviews & Experiments

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Introduction: Cervical cancer is the second most common cancer and the largest cancer killer among women in most developing countries including India. Although, various drugs have been developed for cervical cancer, treatment with these drugs often results in a number of undesirable side effects, toxicity and multidrug resistance (MDR). Also, the outcomes for cervical cancer patients remain poor after surgery and chemo radiation. Methods: A literature search (for drugs and delivery systems against cervical cancer) was performed on PubMed and through Google. The present review discuss about various methods including its current conventional treatment with special reference to recent advances in delivery systems encapsulating various anticancer drugs and natural plant products for targeting towards cervical cancer. The role of photothermal therapy, gene therapy and radiation therapy against cervical cancer is also discussed. Results: Systemic/targeted drug delivery systems including liposomes, nanoparticles, hydrogels, dendrimers etc. and localized drug delivery systems like cervical patches, films, rings etc. are safer than the conventional chemotherapy which has further been proved by the several drug delivery systems undergoing clinical trials. Conclusion: Novel approaches for the aggressive treatment of cervical cancer will optimistically result in decreased side effects as well as toxicity, frequency of administration of existing drugs, to overcome MDR and to increase the survival rates.

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“…[10][11][12][13] Especially, the applications of AgNPs have risen up in cancer diagnosis and treatment, 14 not only as attractive targeted drug delivery vehicles or probes for early cancer screening 15,16 but also as a promising therapeutic molecule by itself. AgNPs showed potential cytotoxicity 18 colon cancer HT29 cells, 19 cervical cancer HeLa cells, 20 and Dalton's lymphoma ascites tumor. 21 Hsin et al provided evidence for the mitochondrial pathway of apoptosis of AgNPs in NIH3T3 cells, which is mediated by a ROS-and JNK-dependent mechanism.…”

Section: Introductionmentioning

confidence: 99%

“…23 AgNPs can selectively inhibit HeLa cells by caspase-3-mediated cell death and DNA damage. 20 Yet, neither the underlying biochemical mechanisms nor the molecular triggers have been revealed. 24 In general, the toxicity of AgNPs appears to be driven by the release of silver ions.…”

Section: Introductionmentioning

confidence: 99%

Effects of green-synthesized silver nanoparticles on lung cancer cells in vitro and grown as xenograft tumors in vivo

He¹,

Du²,

Ma³

et al. 2016

IJN

153

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Silver nanoparticles (AgNPs) have now been recognized as promising therapeutic molecules and are extending their use in cancer diagnosis and therapy. This study demonstrates for the first time the antitumor activity of green-synthesized AgNPs against lung cancer in vitro and in vivo. Cytotoxicity effect was explored on human lung cancer H1299 cells in vitro by MTT and trypan blue assays. Apoptosis was measured by morphological assessment, and nuclear factor-κB (NF-κB) transcriptional activity was determined by a luciferase reporter gene assay. The expressions of phosphorylated stat3, bcl-2, survivin, and caspase-3 were examined by Western blot analysis. AgNPs showed dose-dependent cytotoxicity and stimulation of apoptosis in H1299 cells. The effects on H1299 cells correlated well with the inhibition of NF-κB activity, a decrease in bcl-2, and an increase in caspase-3 and survivin expression. AgNPs significantly suppressed the H1299 tumor growth in a xenograft severe combined immunodeficient (SCID) mouse model. The results demonstrate the anticancer activities of AgNPs, suggesting that they may act as potential beneficial molecules in lung cancer chemoprevention and chemotherapy, especially for early-stage intervention.

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An investigation on the cytotoxicity and caspase-mediated apoptotic effect of biologically synthesized silver nanoparticles using Podophyllum hexandrum on human cervical carcinoma cells

Cited by 274 publications

References 46 publications

Nanosilver: new ageless and versatile biomedical therapeutic scaffold

Nanosilver: new ageless and versatile biomedical therapeutic scaffold

Possible role of nanocarriers in drug delivery against cervical cancer

Effects of green-synthesized silver nanoparticles on lung cancer cells in vitro and grown as xenograft tumors in vivo

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