An Invitation to Bio-Analytical Chemistry

Imai, Kazuhiro

doi:10.2116/analsci.14.257

Cited by 6 publications

(3 citation statements)

References 86 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the future, the novel fluorogenic reagents having the advantages of benzofurazan skeleton, and the more sensitive and useful method for the determination of a trace amount of analytes, will be developed. We believe that these developments will be helpful in clarifying the physiological roles of biological substances in the field of biochemistry and bio-analytical chemistry (Imai, 1998).…”

Section: Resultsmentioning

confidence: 98%

Fluorogenic and fluorescent labeling reagents with a benzofurazan skeleton

Uchiyama

Santa

Okiyama

et al. 2001

Biomedical Chromatography

Self Cite

152

101

View full text Add to dashboard Cite

Fluorogenic and fluorescent labeling reagents having a benzofurazan (2,1,3-benzoxadiazole) skeleton such as 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F), 4-N,N-dimethylaminosulfonyl-7-fluoro-2,1,3-benzoxadiazole (DBD-F), 4-aminosulfonyl-7-fluoro-2,1,3-benzoxadiazole (ABD-F), ammonium 7-fluoro-2,1,3-benzoxadiazole-4-sulfonate (SBD-F), 4-hydrazino-7-nitro-2,1,3-benzoxadiazole (NBD-H), 4-N,N-dimethylaminosulfonyl-7-hydrazino-2,1,3-benzoxadiazole (DBD-H), 4-nitro-7-N-piperazino-2,1,3-benzoxadiazole (NBD-PZ), 4-N,N-dimethylaminosulfonyl-7-N-piperazino-2,1,3-benzoxadiazole (DBD-PZ), 4-(N-chloroformylmethyl-N-methyl)amino-7-N,N-dimethylaminosulfonyl-2,1,3-benzoxadiazole (DBD-COCl) and 7-N,N-dimethylaminosulfonyl-4-(2,1,3-benzoxadiazolyl) isothiocyanate (DBD-NCS) are reviewed in terms of synthetic method, reactivity, fluorescence characteristics, sensitivity and application to analytes.

show abstract

Section: Resultsmentioning

confidence: 98%

Fluorogenic and fluorescent labeling reagents with a benzofurazan skeleton

Uchiyama

Santa

Okiyama

et al. 2001

Biomedical Chromatography

Self Cite

152

101

View full text Add to dashboard Cite

show abstract

“…We will first introduce bio-analytical chemistry ( Imai, 1998 ), which is used by researchers in fields including pharmacology, agriculture, medicine, and biotechnology. Conventional analytical chemists typically develop analytical methods that solve particular problems; however, further application of these methods does not often reach their full potential in fields such as life sciences because the possibilities of these methods are not fully realized or explored.…”

Section: Introductionmentioning

confidence: 99%

Recent Progress in FD-LC-MS/MS Proteomics Method

Kobayashi

Imai

2021

Front. Chem.

Self Cite

View full text Add to dashboard Cite

Through the course of our bio-analytical chemistry studies, we developed a novel proteomics analysis method, FD-LC-MS/MS (fluorogenic derivatization-liquid chromatography-tandem mass spectrometry). This method consists of fluorogenic derivatization (FD), LC separation, and detection/quantification of the derivatized proteins, followed by isolation, tryptic digestion of the isolated proteins, and final identification of the isolated proteins using electrospray ionization nano-LC-MS/MS of the generated peptide mixtures with a probability-based protein identification algorithm. In this review, we will present various examples where this method has been used successfully to identify expressed proteins in individual human cells. FD-LC-MS/MS is also suitable for differential proteomics analysis. Here, two biological samples are treated by the same steps mentioned above, and the two chromatograms obtained are compared to identify peaks with different intensities (variation in protein levels). Associated peak fractions are then isolated, and the differentially expressed proteins between the two samples are identified by LC-MS/MS. Several biomarkers for cancers have been identified by FD-LC-MS/MS. For more efficient separation, nano-flow LC with a phenyl-bonded monolithic silica-based capillary column was adopted for cell-expressed intact protein analysis. The derivatized human cell proteins (K562) and yeast cell (Saccharomyces cerevisiae) proteins as model intact cell proteins were analyzed by nano-flow LC with fluorescence detection. More than 1,300 protein peaks were separated/detected from both cells. For straightforward comparison of multiple peak separation profiles, a novel type of chromatogram display, termed the “spiderweb” chromatogram, was developed. A nano-LC-FD-LC-mass spectrometry trial for molecular weight estimation of FD proteins has also been conducted.

show abstract

“…Key words-benzofurazan reagent; chemiluminescence; HPLC; MS/MS; proteomics; bio-analytical chemistry Detection is the Heart of Analytical Chemistry! The Way to Find the Related Molecule to Maintain the Life ．[1][2][3] …”

mentioning

confidence: 99%

Analytical Chemical Studies on High‐Performance Recognition and Detection of Bio‐Molecules in Life

Imai

2004

ChemInform

Self Cite

View full text Add to dashboard Cite

In order to understand the mechanism for maintaining life of animals based on the search of dynamics of biomolecules, I have developed several sensitive and selective methods for their quantiˆcation. Using the methods of derivatization with the developed benzofurazan ‰uorogenic reagents (4-‰uoro-7-nitro-2,1,3-benzoxadiazole (NBD F), ammonium 7-‰uoro-2,1,3-benzoxadiazole 4-sulfonate (SBD F) and etc.) followed by high-performance liquid chromatography (HPLC)-‰uorescence detection, a certain kind of biological and clinical importances was demonstrated of chiral bio-molecules (D-amino acids, D-lactic acid and so on), peptides and proteins. The proposed method (derivatization with SBD F, isolation of the ‰uorescent proteins by two-dimensional HPLC, enzymatic digestion and identiˆca-tion of the altered proteins by HPLC mass spectrometry (MS)/MS with database-searching algorithm) for proteomics studies revieled the changed proteins in the islets of Langerhans of the dexamethazone-induced diabetic rats. An importance of catecholamine metabolism on the blood pressure regulation was also suggested by the method of HPLC chemiluminescence detection of catecholamines and their 3-O-methylmetabolites. A newˆeld of Analytical Chemistry, i.e., Bio-Analytical Chemistry, was also proposed.

show abstract

An Invitation to Bio-Analytical Chemistry

Cited by 6 publications

References 86 publications

Fluorogenic and fluorescent labeling reagents with a benzofurazan skeleton

Fluorogenic and fluorescent labeling reagents with a benzofurazan skeleton

Recent Progress in FD-LC-MS/MS Proteomics Method

Analytical Chemical Studies on High‐Performance Recognition and Detection of Bio‐Molecules in Life

Contact Info

Product

Resources

About