An ionizable chromophoric reagent for the analysis of primary amine-containing drugs by capillary electrophoresis

Abstract

We found that ofloxacin acyl chloride is a potential chromophoric reagent for labeling amino analytes for capillary electrophoresis. Ofloxacin acyl chloride has a tertiary amino function in its structure and the derivatives from ofloxacin acyl chloride reacting with amino analytes can be ionized by an acid treatment and analyzed by simple capillary zone electrophoresis. Ofloxacin acyl chloride was used to derivatize model analytes (without chromophore) of amantadine (amino drug), tranexamic acid (non-protein amino carboxylic acid), glycine, and methionine (protein amino acids). The resulting derivatives were analyzed by capillary zone electrophoresis with ultraviolet detection (300 nm). The detection limits of the analytes studied were in the range of 1.0-2.5 microM (S/N = 3, injection 3 s). The precision (relative standard deviation) and accuracy (relative error) of the method for intra- and inter-day analyses of the analytes were respectively below 4.5% and 3.9%. Application of the method to the analysis of tranexamic acid in plasma proved feasible.

Plasma and cerebral spinal fluid tranexamic acid quantitation in cardiopulmonary bypass patients

“…Several previous methods for the determination of TXA in plasma have been published using HPLC-UV [5,6], HPLCfluorescence [7][8][9][10][11], GC-MS [12], and electrophoresis [13,14].…”

Recent advances in amino acid analysis by capillary electrophoresis

“…The RSD of the method for intra-and interday analyses of the analytes was below 4.5%. An application to tranexamic acid in plasma is shown [9]. One must note that this strategy is original because positively charged species are obtained, but the reaction is water sensitive.…”

Analytical methods for quantification of tranexamic acid in biological fluids: A review

“…MS detection enables straightforward analyte identification and quantification eliminating the need of any derivatisation procedure, which may introduce large assay variations, namely the stability of the newly formed derivative. Less common applications reporting the determination of TXA include techniques such as capillary electrophoresis and paper electrophoresis coupled to UV-Vis detection [42,43].…”

“…Several protein precipitation reagents have been applied to achieve this goal, specifically picric acid [42,44], heptafluorobutyric acid [33], perchloric acid [25,34,35,43], methanol [37,41], ethanol [26,30], and acetonitrile [24,28,31,39,45,46], which is in fact the most commonly used. Fiechtner et al [28] reported the pre-treatment of plasma samples with leucine dehydrogenase prior to deproteinization by acetonitrile.…”