An iridium oxide nanoparticle and polythionine thin film based platform for sensitive Leishmania DNA detection

Mayorga‐Martinez, Carmen C.; Chamorro-García, Alejandro; Serrano, Lorena; Rivas, Lourdes; Quesada-González, Daniel; Altet, Laura; Francino, Olga; Sánchez, Armand; Merkoçi, Arben

doi:10.1039/c5tb00545k

Cited by 31 publications

(11 citation statements)

References 25 publications

(54 reference statements)

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“…3B). Iridium oxide nanoparticles (IrO2NPs) are other 0D nanomaterials used due to their catalytic properties towards water oxidation reaction 26 , the production of oxygen from water, and their application in impedimetric sensors [27][28] . Impedance is a technique used to measure frequency changes on the dielectric medium close to the nanoparticles.…”

Section: D Electrochemical-based Poc Devicesmentioning

confidence: 99%

Nanomaterial-based devices for point-of-care diagnostic applications

2018

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In this review, we have discussed the capabilities of nanomaterials for point-of-care (PoC) diagnostics and explained how these materials can help to strengthen, miniaturize and improve the quality of diagnostic devices. Since the optical, electrochemical and other physical properties of nanomaterials are dictated by their composition, size and shape, these factors are critical in the design and function of nanomaterial-based PoC diagnostics.

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Section: D Electrochemical-based Poc Devicesmentioning

confidence: 99%

Nanomaterial-based devices for point-of-care diagnostic applications

2018

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“…Earlier publications addressed detection of nucleic acid of infectious agents using voltammetric, amperometric and impedance electrochemical approaches (Hassen et al, 2008;Pournaghu-Azar et al, 2008;Cash et al, 2009;Benvidi et al, 2015). Notably, DNA hybridization biosensors based on electrochemical spectroscopy impedance (EIS) are being developed in both label-free formats and label-based approaches using mostly gold nanoparticle (AuNP) and cadmium sulfide quantum dot (CdS QD) as tags (Wang et al, 2002 and2003;Xu et al, 2004;Pumera et al, 2005;Bonanni et al, 2008;Rasheed and Sandhyarani, 2017 ;Ribovski et al, 2017) also employing other nanomaterials (Wang et al, 2003;De la Escosura-Muñiz and Merkoçi, 2010;Merkoçi, 2010;De la Escosura-Muñiz and Merkoçi, 2014;Mayorga-Martinez et al, 2015;De la Escosura-Muñiz et al, 2016). Modification of electrode surface for immobilization of biorecognition receptor (i.e.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical detection of plant virus using gold nanoparticle-modified electrodes

Khater

Escosura‐Muñiz

Quesada-González

et al. 2019

Analytica Chimica Acta

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112

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Tristeza is one of the destructive diseases of citrus causing by citrus tristeza virus (CTV).Historically, CTV has been associated with serious outbreaks of quick decline of citrus, therefore CTV monitoring is important aspect for avoiding such re-emerging epidemics, which would threat citrus production through the world. In this context, we have designed for the first time a label-free impedimetric biosensor for the detection of nucleic acid of CTV.The sensing platform based on a screen-printed carbon electrode (SPCE) was modified by electrodeposited gold nanoparticles (AuNPs), which allowed to efficiently immobilizing thiolated ssDNA probes as well to enhance the electrode conductivity. The growth of AuNPs was optimized and characterized using scanning electron microscopy (SEM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). We investigated the behavior of thiolated ssDNA probe layer and its hybridization with target DNA onto AuNP surfaces by EIS measurements in Fe(CN6) 4-/Fe(CN6) 3red-ox system. The main sensor design aspects such as AuNPs size, probe DNA concentration and immobilization time together with DNA hybridization time were optimized so as to achieve the best performance. Impedance values of DNA hybridization increased with Citrus tristeza-related synthetic DNA concentration, showing a logarithmic relation in the range of 0.1 to 10 µM. The results also indicate that the biosensor was able to selectively detect CTV nucleic acids in the presence of other non-specific DNAs. Moreover, we have demonstrated the good performance of the system in a real plant sample matrix. In addition, the sensor reproducibility enhanced after the hybridization onto MCH/poly (AT) thiolated DNA probes which was confirmed by intra-and inter-day variability assays.

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“…Therefore, these electrodes are cumbersome to use for electrochemical aptasensing applications. The screen printed carbon electrode (SPCE) based biosensor offers the advantages over the conventional electrochemical biosensors in terms of disposability, portability and feasibility for on-site analysis at remote areas [14][15][16]. The preparation of SPCEs is simple, inexpensive and versatile with advantages of mass production and miniaturization of devices.…”

Section: Introductionmentioning

confidence: 99%