An ISMap 02 -like insertion sequence in Mycobacterium spp. interferes with specific detection of Mycobacterium avium subsp. paratuberculosis

Park, Hong-Tae; Jung, Young-Hoon; Yoo, Han Sang

doi:10.1016/j.vetmic.2018.01.013

Cited by 7 publications

(9 citation statements)

References 36 publications

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“…The target of the PCR kit used in this study is ISMap02. Previous work has shown that PCR reactions targeting this insertion sequence may detect other, non-MAP, species of Mycobacterium (Park et al, 2018).…”

Section: Resultsmentioning

confidence: 99%

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Environmental sampling to assess the bioburden of Mycobacterium avium subspecies paratuberculosis in drylot pens on California dairies

Chamchoy

Williams

Adaska

et al. 2019

PeerJ

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Mycobacterium avium subspecies paratuberculosis (MAP) is a bacterium that can cause substantial economic losses in infected dairy herds due to reduced milk production and increased cow-replacement costs. In order to control MAP in dairies with drylot pens, a standardized environmental sampling protocol to quantify MAP in fecal slurry was developed based on an existing protocol for freestall pens. Specifically, following a 24 h hold of the flush, a grab sample of approximately 10 ml of fecal slurry was collected every 1 m along the flush lane of the drylot pens, avoiding individual cow fecal pats. To determine the reliability and repatability of the new environmental sampling protocol for estimation of MAP bioburden at the pen level, two collectors simultaneously collected fecal slurry samples every day for 3 days from six drylot cow pens on two Central California dairies. During the study period no cow movement between pens was allowed with the exception of sick cows. The study herds had MAP seroprevalence of 5.8% and 3.2%, respectively, based on whole pen serum ELISA results. Variance components models for quantitative real-time PCR (qPCR) results showed samples collected from different pens on different dairies accounted for greater variablitiy in MAP concentration (65%), while samples collected by different collectors had the least variability (0.1%). In contrast, variability in MAP concentration in environmental samples collected on different days had 25% variability. The intraclass correlation coefficient showed high reliability (93%) of environmental sampling simultaneously by different collectors. In contrast, the reliability of environmental sampling at different days was 65%, which was similar to the reliability for sampling by different collectors on different days. Investigators can expect high reliability when employing the new environmental sampling protocol along with qPCR testing of environmental samples from drylot pens.

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Section: Resultsmentioning

confidence: 99%

“…The choice of qPCR testing allowed us to detect the ISMap02 sequence which is specific to MAP. However, another study showed that non-MAP mycobacteria could give positive results by ISMAP02 targeting PCR which could have resulted in false positives (Park et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Environmental sampling to assess the bioburden of Mycobacterium avium subspecies paratuberculosis in drylot pens on California dairies

Chamchoy

Williams

Adaska

et al. 2019

PeerJ

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“…Detection by PCR provides rapid results and is very sensitive, but it cannot distinguish between live and dead MAP [ 71 , 73 ]. In addition, false positive identifications may occur due to identification of non-MAP mycobacteria [ 74 ]. Thus, there is a need for rapid, sensitive, and highly specific detection of MAP, both in livestock and food.…”

Section: Discussionmentioning

confidence: 99%

Mycobacterium avium subsp. paratuberculosis Proteome Changes Profoundly in Milk

Kleinwort¹,

Hobmaier²,

Mayer

et al. 2021

Metabolites

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Mycobacterium avium subspecies paratuberculosis (MAP) are detectable viable in milk and other dairy products. The molecular mechanisms allowing the adaptation of MAP in these products are still poorly understood. To obtain information about respective adaptation of MAP in milk, we differentially analyzed the proteomes of MAP cultivated for 48 h in either milk at 37 °C or 4 °C or Middlebrook 7H9 broth as a control. From a total of 2197 MAP proteins identified, 242 proteins were at least fivefold higher in abundance in milk. MAP responded to the nutritional shortage in milk with upregulation of 32% of proteins with function in metabolism and 17% in fatty acid metabolism/synthesis. Additionally, MAP upregulated clusters of 19% proteins with roles in stress responses and immune evasion, 19% in transcription/translation, and 13% in bacterial cell wall synthesis. Dut, MmpL4_1, and RecA were only detected in MAP incubated in milk, pointing to very important roles of these proteins for MAP coping with a stressful environment. Dut is essential and plays an exclusive role for growth, MmpL4_1 for virulence through secretion of specific lipids, and RecA for SOS response of mycobacteria. Further, 35 candidates with stable expression in all conditions were detected, which could serve as targets for detection. Data are available via ProteomeXchange with identifier PXD027444.

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“…M. virginiense had never been isolated from the human body until Vasireddy, et al [12] reported three isolates from the tendon, elbow, and knee. Since the first report in 2016, only two more M. virginiense isolates, one from a mud specimen of a swine farm in Japan [4] and another from bovine fecal specimens [5], have been reported. We report the first isolation of M. virginiense from a human pulmonary specimen.…”

mentioning

confidence: 99%

“…Of note, the patient works for the Korean Forest Service; thus, he is frequently exposed to mud and soil. Because M. virginiense has been isolated from mud and bovine fecal specimens, its infection in this case may be linked to the patient's professional activities [45].…”

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confidence: 99%