An Isocratic HPLC-ECD Assay of Urinary Normetanephrine and Metanephrine

Kumar, Adarsh; Kumar, Mahendra; Fernandez, Benny; Goodkin, Karl; Schneiderman, Neil; Eisdorfer, Carl

doi:10.1080/10826079508010269

Cited by 6 publications

(6 citation statements)

References 20 publications

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“…In our assay protein was removed from tissues by PCA precipitation. To remove other potential interfering compounds prior to assay, we used alumina N chromatography (18) for DOPEGAL, DOPAL, and other CA metabolites. For the CA an alumina B extraction procedure (7,18) was used in all tissues prior to MHLPC.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Quantitation of 3,4-Dihydroxyphenylacetaldehyde and 3,4-Dihydroxyphenylglycolaldehyde, the Monoamine Oxidase Metabolites of Dopamine and Noradrenaline, in Human Tissues by Microcolumn High-Performance Liquid Chromatography

Burke

Chung

1999

Analytical Biochemistry

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Section: Discussionmentioning

confidence: 99%

“…The supernatants were mixed with from 150 to 400 l of 0.2 M phosphate buffer (pH 8.97) as needed to bring pH to 7.5. The mixtures were applied to alumina N columns (18) and allowed to flow slowly. The columns were washed with 3 to 4 ml of diethyl ether and eluted with 5 to 7 ml of ethyl acetate.…”

Section: Methodsmentioning

confidence: 99%

Quantitation of 3,4-Dihydroxyphenylacetaldehyde and 3,4-Dihydroxyphenylglycolaldehyde, the Monoamine Oxidase Metabolites of Dopamine and Noradrenaline, in Human Tissues by Microcolumn High-Performance Liquid Chromatography

Burke

Chung

1999

Analytical Biochemistry

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“…6 Since the first successful combination of microdialysis and high-performance liquid chromatography (HPLC) in 1980s, 7 the technique has been most widely used in the study of neurotransmitters and their metabolites. Typical detection methods are electrochemical detection (HPLC-EC) 8,9 and fluorescence detection (HPLC-FL).…”

mentioning

confidence: 99%

Simultaneous Determination of Biogenic Monoamines in Rat Brain Dialysates Using Capillary High-Performance Liquid Chromatography with Photoluminescence Following Electron Transfer

et al. 2006

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Simultaneous determination of biogenic monoamines such as dopamine, serotonin, and 3-methoxytyramine in brain is important in understanding neurotransmitter activity. This study presents a sensitive determination of biogenic monoamines in rat brain striatum microdialysates using capillary high-performance liquid chromatography with the photoluminescence following electrontransfer detection technique. Separation conditions were optimized by changing the concentration of an ion-interaction agent and the percentage of an organic modifier. The high concentration of ioninteraction agent enabled the amines as a class to be separated from interfering acids, but also made the separation very long. To shorten the separation time, 10% (v/v) acetonitrile was used as the organic modifier. Eight chromatographic runs during a 3-h period were analyzed in terms of retention times, peak heights, and peak widths. Chromatograms are very reproducible, with less than 1% changes in peak height over 3 h. Typical concentration detection limits at the optimum separation conditions were less than 100 pM for metabolic acids and ~200 pM for monoamines. The injection volume of the sample was 500 nL. Thus, the mass detection limits were less than 50 amol for metabolic acids and ~100 amol for monoamines. Typical separation time was less than 10 min. To validate the technique, the separation method was applied to the observation of drug-induced changes of monoamine concentrations in rat brain microdialysis samples. Local perfusion of tetrodotoxin, a sodium channel blocker, into the striatum of an anesthetized rat decreased dopamine, 3-methoxytyramine, and serotonin concentrations in dialysates. Successive monitoring of striatal dialysates at a temporal resolution of 7.7 min showed that the injection of nomifensine transiently increased dopamine and 3-methoxytyramine concentrations in rat brain dialysate.Biogenic monoamines, such as dopamine (DA) and serotonin (5HT), play important roles as major neurotransmitters in the mammalian central nervous system. 1 Reliable measurement of extracellular neurotransmitter concentration is thus important in understanding the central nervous system and its underlying physiology.Several methods have been used to monitor the extracellular level of neurotransmitters and their metabolites without separation. 2-5 However, some neurons do not contain only one neurotransmitter; thus, changes in the neurotransmitter concentrations are often interrelated. Moreover, the real biological response is more likely due to the interplay of all the released neurotransmitters. Therefore, it is highly desirable to monitor the level of several neurotransmitters simultaneously. Measuring extracellular concentrations of neurotransmitter metabolites, including 3, 4-dihydroxyphenylacetic acid (DOPAC), 3-methoxytyramine * To whom correspondence should be addressed. E-mail: sweber@pitt.edu.. (3MT), 5-hydroxyindole-3-acetic acid (5HIAA), and homovanillic acid (HVA), is also important in understanding the metabolic pathways and ...

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“…Several analytical methods have been published for the determination of HVA in different biological matrices including urine [16,17], cerebro-spinal fluid [18], brain tissues [19], animal plasma [20,21] and other tissues [22]. Other methods regard the analysis of this compound in human plasma; the vast majority of them are based on the use of HPLC with electrochemical [15,21,[23][24][25][26][27][28][29] detection, however at least one uses HPLC with fluorescence detection [17] and another capillary gas chromatography-mass spectrometry [30].…”

Section: Introductionmentioning

confidence: 98%

“…Moreover, HVA levels in human blood can be used as marker for neuroblastoma [11] and they have been found to be elevated in plasma of patients with chronic schizophrenia treated with atypical antipsychotic drugs [12][13][14]. The study of HVA plasma level variability is therefore very important for the diagnosis of disorders and behavioural problems [15]; consequently, there is a constant strive for the development of reliable, sensitive and precise methods for the determination of this compound in plasma samples. Several analytical methods have been published for the determination of HVA in different biological matrices including urine [16,17], cerebro-spinal fluid [18], brain tissues [19], animal plasma [20,21] and other tissues [22].…”

Section: Introductionmentioning

confidence: 99%

Determination of homovanillic acid (HVA) in human plasma by HPLC with coulometric detection and a new SPE procedure

Saracino

Mandrioli

Mercolini

et al. 2006

Journal of Pharmaceutical and Biomedical Analysis

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An Isocratic HPLC-ECD Assay of Urinary Normetanephrine and Metanephrine

Cited by 6 publications

References 20 publications

Quantitation of 3,4-Dihydroxyphenylacetaldehyde and 3,4-Dihydroxyphenylglycolaldehyde, the Monoamine Oxidase Metabolites of Dopamine and Noradrenaline, in Human Tissues by Microcolumn High-Performance Liquid Chromatography

Quantitation of 3,4-Dihydroxyphenylacetaldehyde and 3,4-Dihydroxyphenylglycolaldehyde, the Monoamine Oxidase Metabolites of Dopamine and Noradrenaline, in Human Tissues by Microcolumn High-Performance Liquid Chromatography

Simultaneous Determination of Biogenic Monoamines in Rat Brain Dialysates Using Capillary High-Performance Liquid Chromatography with Photoluminescence Following Electron Transfer

Determination of homovanillic acid (HVA) in human plasma by HPLC with coulometric detection and a new SPE procedure

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