An Isoform of the Eukaryotic Translation Elongation Factor 1A (eEF1a) Acts as a Pro-Viral Factor Required for Tomato Spotted Wilt Virus Disease in Nicotiana benthamiana

Helderman, Tieme A.; Deurhof, Laurens; Bertran, André; Boeren, Sjef; Fokkens, Like; Kormelink, Richard; Joosten, M.H.A.J.; Prins, Marcel; Burg, H.A. van den

doi:10.3390/v13112190

Cited by 6 publications

(10 citation statements)

References 93 publications

(121 reference statements)

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“…Several host factors interacting with TSWV have been identified, one such factor being the eukaryotic translation elongation factor 1A (eEF1A). eEF1A interacts with the RNP of TSWV, and silencing eEF1A via virus-induced gene silencing (VIGS) significantly inhibits TSWV replication in tobacco [153,154]. Ribosomal protein S6 (RPS6) is a host factor that is part of the 40S ribosomal subunit.…”

Section: Crispr/cas-mediated Tomato Breeding For Resistance Against O...mentioning

confidence: 99%

Advances and Prospects of Virus-Resistant Breeding in Tomatoes

Shahriari,

Su,

Zheng

et al. 2023

IJMS

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Plant viruses are the main pathogens which cause significant quality and yield losses in tomato crops. The important viruses that infect tomatoes worldwide belong to five genera: Begomovirus, Orthotospovirus, Tobamovirus, Potyvirus, and Crinivirus. Tomato resistance genes against viruses, including Ty gene resistance against begomoviruses, Sw gene resistance against orthotospoviruses, Tm gene resistance against tobamoviruses, and Pot 1 gene resistance against potyviruses, have been identified from wild germplasm and introduced into cultivated cultivars via hybrid breeding. However, these resistance genes mainly exhibit qualitative resistance mediated by single genes, which cannot protect against virus mutations, recombination, mixed-infection, or emerging viruses, thus posing a great challenge to tomato antiviral breeding. Based on the epidemic characteristics of tomato viruses, we propose that future studies on tomato virus resistance breeding should focus on rapidly, safely, and efficiently creating broad-spectrum germplasm materials resistant to multiple viruses. Accordingly, we summarized and analyzed the advantages and characteristics of the three tomato antiviral breeding strategies, including marker-assisted selection (MAS)-based hybrid breeding, RNA interference (RNAi)-based transgenic breeding, and CRISPR/Cas-based gene editing. Finally, we highlighted the challenges and provided suggestions for improving tomato antiviral breeding in the future using the three breeding strategies.

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Section: Crispr/cas-mediated Tomato Breeding For Resistance Against O...mentioning

confidence: 99%

Advances and Prospects of Virus-Resistant Breeding in Tomatoes

Shahriari,

Su,

Zheng

et al. 2023

IJMS

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“…The interaction between the ER-localized P3 from the Soybean mosaic virus (SMV) and eEF1α has been shown to be essential for virulence in the susceptible host [82]. Furthermore, eEF1A from N. benthamiana has been identified as a pro-viral factor required for the Tomato spotted wilt virus disease (TSWV) [92]. Maize possesses at least eleven eEFs, from which nine are annotated in the MaizeGDB database as "elongation factor α (elfα)" (Supplementary Table 1).…”

Section: The Presence Of Diverse Transcripts For Eifs and Eefs Imply A Complex Regulation Of Translation In Scmv Interaction With Maize Tmentioning

confidence: 99%

The Prominent Role of Translation Factors, Heat Shock Protein 90-2 and an ABC Transporter Associated to the Resistance of a Maize Line Against Sugarcane Mosaic Virus

Rodríguez-Gómez¹,

Vargas-Mejía²,

Silva-Rosales³

2022

Preprint

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The mosaic disease in maize is caused by Sugarcane mosaic virus (SCMV), a member of the Potyviridae family. The best strategy to cope with viral infections is the use of disease-resistant maize lines. To better understand the resistance response to SCMV, we analyzed differentially expressed genes among a resistant line (CI-RL1), a susceptible line (B73), and the F1 progeny from a cross between both lines using RNA-Seq data. We also analyzed transcript expression pattern clustering to allocate previously reported resistance candidate genes. GO enrichment analysis of biological processes highlighted a strong regulation in ROS detoxification in both the susceptible and resistant lines. The enrichment of cellular components led to the identification of an integral component of the plasma membrane in the RL line. Transcript expression patterns provide evidence of the importance of host translation in virus response, showing the diverse and complex behavior of eIF4E homologs and the presence of eleven eEF1α factors in maize. In addition, we identified two genes putatively implied in long-distance movement: ZmPiezo and ZmPVIP1. Finally, we propose an ABC transporter to be associated with viral resistance.

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“…T.A. Helderman et al [ 4 ] studied host factors that are important for the systemic spread of Tomato spotted wilt orthotospovirus (TSWV) and disease symptom development. Their data indicate that silencing one clade of the eEF1A gene family strongly suppressed TSWV accumulation and disease symptom development in N. benthamiana , suggesting that the gene products act as important susceptibility factors for TSWV.…”

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confidence: 99%