An isothermal lab-on-phone test for easy molecular diagnosis of SARS-CoV-2 near patients and in less than 1 hour

Dória, Gonçalo; Clemente, Carla; Coelho, E.; Colaço, João; Crespo, Rui; Semikhodskii, Andrei; Mansinho, Hélder; DinisCarvalho, MagnoMaria Fernanda; Casmarrinha, Manuela; Samina, Cátia; Vidal, Ana Cristina; Delarue, Francisca; Graúdo, Susana; Santos, Ana Catarina; Peças, David; Carreira, Olga; Marques, Raquel; Gaspar, Carina; Flores, Orfeu

doi:10.1016/j.ijid.2022.07.042

Cited by 7 publications

(6 citation statements)

References 15 publications

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“…The device has been used successfully for on-site detection of SARS-CoV-2. 30 Compared to other portable or smartphonebased fluorescence detection instruments, 51,52 the Doctor Vida pocket test offers control of the temperature and real-time monitoring of the fluorescence profile. Thus, this technique should be generally suitable for the detection of LAMP assays in the field.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…The Doctor Vida pocket test is a pocket-sized device for isothermal amplification and allows for easy and rapid on-site measurement of fluorescence. The device has been used successfully for on-site detection of SARS-CoV-2 . Compared to other portable or smartphone-based fluorescence detection instruments, , the Doctor Vida pocket test offers control of the temperature and real-time monitoring of the fluorescence profile.…”

Section: Resultsmentioning

confidence: 99%

“…The progress of amplification can be monitored via a mobile app (Dr Vida Pocket PCR). 30 The isothermal lab-on-phone assay offers the advantage of real-time monitoring, simple and user-friendly operation, and more informative results than LFAs. Both platforms have the potential to enable in-field detection of fish species and thus contribute to compliance with current legislation and extend already existing in-field readout systems for Pleuronectiformes.…”

Section: ■ Introductionmentioning

confidence: 99%

“…The portable and hand-held device allows isothermal amplification of any genetic material and can measure fluorescence in real time. The progress of amplification can be monitored via a mobile app (Dr Vida Pocket PCR) . The isothermal lab-on-phone assay offers the advantage of real-time monitoring, simple and user-friendly operation, and more informative results than LFAs.…”

Section: Introductionmentioning

confidence: 99%

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Fast and User-Friendly Detection of Flatfish Species (Pleuronectes platessa and Solea solea) via Loop-Mediated Isothermal Amplification (LAMP)

Wax,

Pförtner,

Holz

et al. 2023

J. Agric. Food Chem.

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The detection of a Cytochrome b gene (cytb) for species differentiation in fish is intensively used. A fast alternative to expensive and time-consuming DNA barcoding is loop-mediated isothermal amplification (LAMP) in combination with efficient readout systems. For this reason, we developed LAMP assays for rapid species detection of Pleuronectes platessa and Solea solea, two economically important flatfish species in Europe that are prone to mislabeling. Species-specific primer sets targeting cytb were designed, and LAMP assays were optimized. With the optimized LAMP assays, we were able to detect up to 0.1 and 0.01 ng of target DNA of P. platessa and S. solea, respectively, and in each case up to 1% (w/w) of target species in mixtures with nontarget species. For future on-site detection, a lateral flow assay and a pocket-sized lab-on-phone assay were used as readout systems. The lab-on-phone assay with the S. solea specific primer set revealed cross-reactivity to Solea senegalensis. The assay targeting P. platessa proved to be highly specific. Both assays could be performed within 45 min and provided rapid and easy detection of fish species.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Fast and User-Friendly Detection of Flatfish Species (Pleuronectes platessa and Solea solea) via Loop-Mediated Isothermal Amplification (LAMP)

Wax,

Pförtner,

Holz

et al. 2023

J. Agric. Food Chem.

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“…Although there are already portable real-time fluorometers for reaction monitoring available, e.g., a LAMP-based assay for the detection of COVID-19, the assays developed for this purpose require the investment in such a device as it is not yet standard equipment [24]. However, the use of different visual detection methods is sufficient to provide a clear yes/no answer to the question of the possible contamination of a sample.…”

Section: Introductionmentioning

confidence: 99%

Food Authentication: The Detection of Arbutus unedo and Olea europaea Leaves as an Admixture of Oregano Using LAMP- and Duplex LAMP-Based Test Systems with Lateral-Flow Assays

Holz,

Wax,

Illarionov

et al. 2024

Agriculture

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The Mediterranean herb oregano is one of the most frequently adulterated foods. Often morphologically similar leaf material is used as a filler, which can generally be detected using DNA-based methods. Loop-mediated isothermal amplification (LAMP) has high potential for point-of-care testing as it requires only a simple device for sample incubation and is less sensitive to inhibition by co-isolated metabolites compared to conventional PCRs (polymerase chain reactions). In this work, we have developed two LAMP assays for the specific detection of the adulterants olive (Olea europaea) and strawberry tree (Arbutus unedo). The combination with a rapid isolation protocol and LFAs (Lateral-flow assays) as a visualization technique provides a reliable indication of possible adulteration. It has also been shown that it is possible to estimate the level of contamination and to perform the LAMP/LFA assay with DNA isolation in less than 30 min. As a further option, a duplex LAMP/LFA assay was developed that allows both contaminants to be detected in parallel, making the rapid test system even more cost-effective and user-friendly.

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Laboratory-based molecular test alternatives to RT-PCR for the diagnosis of SARS-CoV-2 infection

Arevalo-Rodriguez,

Mateos-Haro,

Dinnes

et al. 2024

Cochrane Database of Systematic Reviews

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An isothermal lab-on-phone test for easy molecular diagnosis of SARS-CoV-2 near patients and in less than 1 hour

Cited by 7 publications

References 15 publications

Fast and User-Friendly Detection of Flatfish Species (Pleuronectes platessa and Solea solea) via Loop-Mediated Isothermal Amplification (LAMP)

Fast and User-Friendly Detection of Flatfish Species (Pleuronectes platessa and Solea solea) via Loop-Mediated Isothermal Amplification (LAMP)

Food Authentication: The Detection of Arbutus unedo and Olea europaea Leaves as an Admixture of Oregano Using LAMP- and Duplex LAMP-Based Test Systems with Lateral-Flow Assays

Laboratory-based molecular test alternatives to RT-PCR for the diagnosis of SARS-CoV-2 infection

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