An LC-MS/MS based candidate reference method for the quantification of carbamazepine in human serum

Taibon, Judith; Schmid, Rupert; Lucha, Stephanie; Pongratz, Stephan; Tarasov, Kirill V.; Seger, Christoph; Timm, Christian; Thiele, Roland; Herlan, Joachim Mark; Kobold, Uwe

doi:10.1016/j.cca.2017.07.013

Cited by 26 publications

(7 citation statements)

References 22 publications

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“…8 In the past, chromatographic assays with high selectivity, sensitivity, resolution, precision and accuracy have been widely applied in the individual or simultaneous determination of CBZ or/and LTG in biological samples. [9][10][11][12][13][14] However, effective and exclusive extraction, separation and enrichment of CBZ and LTG in biological matrix are still a key topic in chromatographic analysis. Except for conventional protein precipitation using methanol, acetonitrile or acetone as precipitant, solid phase extraction, especially based on molecularly imprinted polymers (MIPs), has attracted more attentions.…”

Section: Introductionmentioning

confidence: 99%

Development of magnetic molecularly imprinted polymers with double templates for the rapid and selective determination of carbamazepine and lamotrigine in serum

et al. 2022

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Section: Introductionmentioning

confidence: 99%

Development of magnetic molecularly imprinted polymers with double templates for the rapid and selective determination of carbamazepine and lamotrigine in serum

et al. 2022

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“…The major analytical tools of metabolomics include gas chromatography–mass spectrometry (GC–MS) [ 10 ], liquid chromatography-mass spectrometry (LC-MS), and nuclear magnetic resonance (NMR) spectroscopy. Various samples from the human body (such as cells, blood, urine, and cerebrospinal fluid) can be studied by using these novel and rapid analysis techniques to aid the assessment of various diseases [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Urinary Metabolomics Study of Patients with Gout Using Gas Chromatography-Mass Spectrometry

Sang

et al. 2018

BioMed Research International

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Objectives Gout is a common type of inflammatory arthritis. The aim of this study was to detect urinary metabolic changes in gout patients which may contribute to understanding the pathological mechanism of gout and discovering potential metabolite markers. Methods Urine samples from 35 gout patients and 29 healthy volunteers were analyzed by gas chromatography–mass spectrometry (GC–MS). Orthogonal partial least-squares discriminant analysis (OPLS-DA) was performed to screen differential metabolites between two groups, and the variable importance for projection (VIP) values and Student's t-test results were combined to define the significant metabolic changes caused by gout. Further, binary logistic regression analysis was performed to establish a model to distinguish gout patients from healthy people, and receiver operating characteristic (ROC) curve was made to evaluate the potential for diagnosis of gout. Result A total of 30 characteristic metabolites were significantly different between gout patients and controls, mainly including amino acids, carbohydrates, organic acids, and their derivatives, associated with perturbations in purine nucleotide synthesis, amino acid metabolism, purine metabolism, lipid metabolism, carbohydrate metabolism, and tricarboxylic acid cycle. Binary logistic regression and ROC curve analysis showed the combination of urate and isoxanthopterin can effectively discriminate the gout patients from controls with the area under the curve (AUC) of 0.879. Conclusion Thus, the urinary metabolomics study is an efficient tool for a better understanding of the metabolic changes of gout, which may support the clinical diagnosis and pathological mechanism study of gout.

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“…The oral pharmacokinetic study on SD rats of the plasma sample concentration of 13d was analyzed by the fully validated method on a liquid chromatography-tandem mass spectrometry (LC–MS/MS) instrument consisting of an auto sampler, Water’s quaternary pump-600 LC system (Waters Milford USA) equipped with a pump, degasser, coupled to an API-3200 mass spectrometer (AB Sciex, Canada). The detection mode of the analyte was positive, using electrospray ionization operated in compound selective reaction monitoring (SRM) at m / z transition 459.2 → 134.1 ( 13d ), and SRM of an internal standard used 237 → 194.1 (Carbamazepine) were chromatographically achieved on Waters symmetry, C18 (150 * 4.6 mm i.d., 5 μm) using binary mobile phase aqueous 0.1% formic acid in triple distilled water and organic acetonitrile at a 35:65 ratio, respectively. The flow rate of the mobile phase was 0.5 mL/min while 20 μL of sample volume injected in LC–MS/MS coupled with a HPLC autosampler followed by the extraction of the protein precipitation (PPT) method and plasma (50 μL) was precipitated with 150 μL of acetonitrile containing an internal standard (IS-50 ng/mL).…”

Section: Experimental Sectionmentioning

confidence: 99%

Tyrosine-Derived Novel Benzoxazine Active in a Rat Syngenic Mammary Tumor Model of Breast Cancer

et al. 2021

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In continuing efforts of improving benzoxazepine derivatives as an anti-breast cancer agent, a new chemical entity, benzoxazine, was designed from scaffold morphing. Structure–activity relationship studies revealed that H, −OMe, −CF3, and −F were well tolerated on R1 and R2 positions of ring A, and R2 as −CH2CH2N(CH2)4 (N-ethyl pyrrolidine) and −CH2CH2N(CH2)5 (N-ethyl piperidine) chains on ring D increased activities (Series B, Figure 3). 13d selected as a lead compound (IC50: 0.20 to 0.65 μM) induces apoptosis, cell cycle arrest, and loss of mitochondrial membrane potential in breast cancer cells. Compound 13d was formulated into 13d–f using cyclodextrin to improve its solubility for a pharmacokinetic, in vivo efficacy study. Both 13d and 13d–f regressed tumor growth at concentrations of 5 and 20 mg/kg better than tamoxifen without any mortality in a rat syngenic mammary tumor model. Collectively, our data suggest that tyrosine-derived novel benzoxazine 13d could be a potential lead for the treatment of breast cancer and hence deserve further in-depth studies.

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An LC-MS/MS based candidate reference method for the quantification of carbamazepine in human serum

Cited by 26 publications

References 22 publications

Development of magnetic molecularly imprinted polymers with double templates for the rapid and selective determination of carbamazepine and lamotrigine in serum

Development of magnetic molecularly imprinted polymers with double templates for the rapid and selective determination of carbamazepine and lamotrigine in serum

Urinary Metabolomics Study of Patients with Gout Using Gas Chromatography-Mass Spectrometry

Tyrosine-Derived Novel Benzoxazine Active in a Rat Syngenic Mammary Tumor Model of Breast Cancer

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