An Lc- MS/MS Method for the Determination of Omeprazole on Proton Pump Inhibitor in Human Plasma

Abstract

A sensitive and selective liquid chromatographic method coupled with tandem mass spectroscopy (LC-MS/MS) was developed for the quantification of omeprazole in human plasma. Lansoprazole was used as internal standard with plasma samples, extracted using 10mM ammonium acetate. A centrifuged upper layer was then evaporated and reconstituted with Acetonitrile: mobile phase buffer 70:30%v/v. The reconstructed samples were injected into a C18 column purospher star 5µ. The mobile phase was composed of ACN: mobile phase buffer (5mm ammonium bicarbonate buffer) in the ratio of 70:30%v/v with flow rate 1.0mL/min. The mass spectrometer was operated using positive ion mode and turbo electro spray ionisation. Nitrogen was used as the nebulizer, curtain, collision and auxiliary gases. Using MS/MS with multiple reactions monitoring (MRM) mode, omeprazole was detected without severe interferences from plasma matrix. Detection of omeprazole in human plasma was accurate and precision. This method has been successfully applied to the study of omeprazole in human specimens

Validated Simultaneous Gradient Ultra-Performance Liquid Chromatographic Quantification of Some Proton Pump Inhibitor Drug Residues in Saudi Pharmaceutical Industrial Wastewater

“…Additionally, actual wastewater samples were carefully treated using the optimized sample preparation technique, then they were chromatographed by applying the optimized chromatographic parameters ( Figure 2 ). The results were compared to those obtained by analyzing the actual wastewater samples using reference methods for the determination of OMP, PNT, and LNZ [ 9 , 17 , 25 ] after sample pretreatment by the same sample preparation protocol ( Table 4 ). Method performance for the determination of the cited drugs was also validated by the application of the standard addition procedure.…”

“…Liquid chromatography coupled with tandem mass spectroscopy (LC-MS/MS) was applied for the determination of OMP in human plasma. It was detected using multiple reaction monitoring (MRM) mode [ 9 ]. Ultra-performance liquid chromatography (UPLC) was applied for the determination of OMP, either simultaneously with domperidone in capsules [ 10 ] or with its related substances using TOF/MS detection [ 11 ].…”

Determination of omeprazole via fluorescence‐quenching methodology; application to content uniformity testing

“…Variable analytical methods have been previously performed for the determination of OMP, including ultraviolet-visible (UV-vis) spectrophotometry [3][4][5][6], spectrofluorimetry [7][8][9], electrochemical methods [10][11][12][13], electrophoresis [14][15][16], high-performance liquid chromatography (HPLC) [17][18][19][20][21], ultrahigh-performance liquid chromatography (UPLC) [22] and liquid chromatography-tandem mass spectrometry (LC-MS/MS) [23].…”

Development and Validation of Stability-Indicating Liquid Chromatographic Assay for Rifaximin (An Antibiotic) in Bulk and Pharmaceutical Dosage Forms

“…Compared with other antibiotics, RFX has a lower rate of fecal pathogenic eradication, so depletion of normal gastrointestinal flora is reduced 5 . Methods reported for the determination of RFX in pharmaceutical dosage forms and biological fluids include RP-HPLC [6][7][8][9] , LC-MS [10][11][12][13] and spectrophotometric [14][15] methods have been developed for the determination of RFX in pharmaceutical formulations and biological fluids. Impurity profiles of Rifaximin were also studied by using Diagnostic fragment-ion-based extension strategy (DFIBES) and derivative resolution of UV spectra [16][17] .…”