An LC–MS–MS Method for the Simultaneous Quantitation of Acyclovir and Valacyclovir in Human Plasma

Kanneti, Ravi; Rajesh, R; Raj, Jog; Bhatt, Parloop

doi:10.1365/s10337-009-1171-3

Cited by 28 publications

(19 citation statements)

References 31 publications

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“…The extract was centrifuged at 14000 rpm for 10 min at −5°C and the concentration of acyclovir was determined by LC-MS/MS using a sample injection volume of 10 μl. The bioanalytical method was modified from previously reported methods (16,19,20). Validation data are included as supplementary material.…”

Section: Methodsmentioning

confidence: 99%

The Influence of Chitosan on the Oral Bioavailability of Acyclovir—a Comparative Bioavailability Study in Humans

et al. 2015

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PurposeThe effects of chitosan hydrochloride on the oral absorption of acyclovir in humans were studied to confirm the absorption enhancing effects reported for in vitro and rat studies, respectively.MethodsA controlled, open-label, randomized, 3-phase study was conducted in 12 healthy human volunteers. Zovirax 200 mg dispersible tablets co-administered with doses of 400 and 1000 mg chitosan HCl were compared with Zovirax only.ResultsThe expected increased absorption of acyclovir was not observed. On the contrary, mean area under the plasma concentration-time curve (AUC0-12 h) and maximal plasma concentration (Cmax) decreased following concomitant chitosan intake (1402 versus 1017 and 982.0 ng∙h/ml and 373 versus 208 and 235 ng/ml, respectively). In addition, Tmax increased significantly in presence of 1000 mg of chitosan from 1 to 2 h.ConclusionsThe results of this study in human volunteers did not confirm an absorption enhancing effect of chitosan. Reference values were comparable to literature data, whereas addition of chitosan resulted in significant opposite effects on Cmax, Tmax and AUC. Additional studies are needed to investigate the cause of the discrepancy. The observed variability and complex potential interactions may complicate the use of chitosan HCl in oral pharmaceutical formulations.Electronic supplementary materialThe online version of this article (doi:10.1007/s11095-014-1613-y) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

The Influence of Chitosan on the Oral Bioavailability of Acyclovir—a Comparative Bioavailability Study in Humans

et al. 2015

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“…Existing analytical methods for acyclovir are based on high-performance liquid chromatography (HPLC) with ultraviolet (Bahrami, Mirzaeei, & Kiani, 2005;Hasan, Chander, Ali, Baboota, & Ali, 2011) or fluorescence detection (Dao, Jiao, & Zhong, 2008), but these methods lack both selectivity and sensitivity. More recent methods that are based on HPLC-tandem mass spectrometry (HPLC-MS/MS) in combination with protein precipitation or solid-phase extraction (SPE) show improved sensitivity, which results in lower limits of quantification (LLOQ) (Holkar, Daphal, Yadav, & Rokade, 2012;Kanneti, Rajesh, Aravinda Raj, & Bhatt, 2009;Rigo-Bonnin et al, 2014;Shao, 2012). These methods, however, are not suitable for the determination of acyclovir in dOFM samples because they require high sample volumes for the sample preparation process.…”

Section: Introductionmentioning

confidence: 99%

Quantification of acyclovir in dermal interstitial fluid and human serum by ultra‐high‐performance liquid–high‐resolution tandem mass spectrometry for topical bioequivalence evaluation

Schimek

Raml

Francesconi

et al. 2018

Biomedical Chromatography

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Time-concentration curves for the topical anti-viral drug acyclovir can provide valuable information for drug development. Open flow microperfusion is used for continuous sampling of dermal interstitial fluid but it requires validated methods for subsequent sample analysis. Therefore, we developed a sensitive, selective and high-throughput ultra-high-performance liquid chromatography-high-resolution tandem mass spectrometry method to determine acyclovir in human dermal interstitial fluid and serum. We validated the method over a concentration range of 0.1-25 ng/mL for a sample volume of just 20 μL and employed cation-exchange solid-phase extraction in a fully automated sample treatment procedure. Short- and long-term sample stability data and the analysis of 5000 samples from a clinical trial demonstrate the successful application of our method.

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“…It has previously been reported that VCV was determined by reversed phase liquid chromatography ,, liquid chromatography with mass spectrometric detection (LC–MS‐MS) ,, micellar electrokinetic chromatography , Spectrophotometric determination ,, as well as voltammetic analysis .…”

Section: Introductionmentioning

confidence: 99%

Voltammetric Determination of Valaciclovir Using a Molecularly Imprinted Polymer Modified Carbon Paste Electrode

et al. 2017

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A molecularly imprinted polymer (MIP) was rationally synthesized with the aid of computer based studies. The computational studies were used to screen for the most suitable template to functional monomer molar ratio. Two functional monomers were involved in the study (methacrylic acid and 4‐vinylpyridine). Four MIP ratios were synthesized in accordance with the results of the computational studies and their performance was evaluated using equilibrium rebinding assays. The MIP with the best performance was used as an additive in carbon paste electrodes for the voltammetric determination of valaciclovir (VCV). Following the optimization of voltammetric parameters, a linear response was obtained in the range of 1.0x10−6–7.0x10−4 M with a limit of detection at 4.45x10−7 M. The MIP modified carbon paste electrode was successfully applied for the determination of VCV in pure solutions and dosage form.

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An LC–MS–MS Method for the Simultaneous Quantitation of Acyclovir and Valacyclovir in Human Plasma

Cited by 28 publications

References 31 publications

The Influence of Chitosan on the Oral Bioavailability of Acyclovir—a Comparative Bioavailability Study in Humans

The Influence of Chitosan on the Oral Bioavailability of Acyclovir—a Comparative Bioavailability Study in Humans

Quantification of acyclovir in dermal interstitial fluid and human serum by ultra‐high‐performance liquid–high‐resolution tandem mass spectrometry for topical bioequivalence evaluation

Voltammetric Determination of Valaciclovir Using a Molecularly Imprinted Polymer Modified Carbon Paste Electrode

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