An MspI polymorphism in the inhibin alpha gene and its associations with superovulation traits in Chinese Holstein cows

Tang, Kai-Fu; Li, Shujing; Yang, Wenguang; Yu, Jiaojiao; Han, Li; Li, Xiang; Yang, Liguo

doi:10.1007/s11033-010-0072-8

Cited by 25 publications

(27 citation statements)

References 24 publications

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“…Furthermore, three of the SNP were found to be expressed within the ovary (Table 3). In particularly, INHA (inhibin alpha) which has various polymorphisms associated with Holstein cow response to superovulation and is well known for its role in regulating follicle stimulating hormone secretion [78]. This gene was also reported in the GWAS-gene identification efforts of Fortes et al [11].…”

Section: Resultsmentioning

confidence: 96%

Multi-Tissue Omics Analyses Reveal Molecular Regulatory Networks for Puberty in Composite Beef Cattle

et al. 2014

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Puberty is a complex physiological event by which animals mature into an adult capable of sexual reproduction. In order to enhance our understanding of the genes and regulatory pathways and networks involved in puberty, we characterized the transcriptome of five reproductive tissues (i.e. hypothalamus, pituitary gland, ovary, uterus, and endometrium) as well as tissues known to be relevant to growth and metabolism needed to achieve puberty (i.e., longissimus dorsi muscle, adipose, and liver). These tissues were collected from pre- and post-pubertal Brangus heifers (3/8 Brahman; Bos indicus x 5/8 Angus; Bos taurus) derived from a population of cattle used to identify quantitative trait loci associated with fertility traits (i.e., age of first observed corpus luteum (ACL), first service conception (FSC), and heifer pregnancy (HPG)). In order to exploit the power of complementary omics analyses, pre- and post-puberty co-expression gene networks were constructed by combining the results from genome-wide association studies (GWAS), RNA-Seq, and bovine transcription factors. Eight tissues among pre-pubertal and post-pubertal Brangus heifers revealed 1,515 differentially expressed and 943 tissue-specific genes within the 17,832 genes confirmed by RNA-Seq analysis. The hypothalamus experienced the most notable up-regulation of genes via puberty (i.e., 204 out of 275 genes). Combining the results of GWAS and RNA-Seq, we identified 25 loci containing a single nucleotide polymorphism (SNP) associated with ACL, FSC, and (or) HPG. Seventeen of these SNP were within a gene and 13 of the genes were expressed in uterus or endometrium. Multi-tissue omics analyses revealed 2,450 co-expressed genes relative to puberty. The pre-pubertal network had 372,861 connections whereas the post-pubertal network had 328,357 connections. A sub-network from this process revealed key transcriptional regulators (i.e., PITX2, FOXA1, DACH2, PROP1, SIX6, etc.). Results from these multi-tissue omics analyses improve understanding of the number of genes and their complex interactions for puberty in cattle.

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Section: Resultsmentioning

confidence: 96%

Multi-Tissue Omics Analyses Reveal Molecular Regulatory Networks for Puberty in Composite Beef Cattle

et al. 2014

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“…The injections were given at 12-h intervals in a dose step-down manner -i.e., doses of 5.4, 4.4, 3.4, and 2.4 mL on each day -as described in previous studies (Tang et al, 2011). Each cow was twice given 4 mL 0.15 mg/mL prostaglandin F2α (Qilu Animal Health Products Co., Ltd., China) along with the sixth and seventh FSH treatments.…”

Section: Superovulation Procedures and Embryos Harvestmentioning

confidence: 99%

Polymorphisms of the bovine growth differentiation factor 9 gene associated with superovulation performance in Chinese Holstein cows

Tang¹,

Li²,

Yang³

2013

Genet. Mol. Res.

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ABSTRACT. Growth differentiation factor 9 (GDF9) belongs to the transforming growth factor β superfamily and plays a critical role in ovarian follicular development and ovulation rate. We examined the bovine GDF9 gene polymorphism and analyzed its association with superovulation performance. Based on the sequence of the bovine GDF9 gene, six pairs of primers were designed to detect single nucleotide polymorphisms of two exons and intron 1 of GDF9 using polymerase chain reaction-single-strand conformation polymorphism. Only the products amplified by primer 3-1 displayed polymorphisms. Sequencing revealed two mutations of A485T and A625T in intron 1 of the GDF9 gene in 171 Chinese Holstein cows treated for superovulation. Association analysis showed that these two single nucleotide polymorphisms of A485T and A625T had significant effects Effects of GDF9 variants on superovulation performance on the number of transferable embryos (P < 0.05), and the A625T polymorphism was significantly associated with the total number of ova (P < 0.05). In addition, a significant additive effect on the number of transferable embryos was detected in polymorphisms of A485T (P < 0.05). This study is the first to identify two polymorphisms in bovine GDF9 and describe their correlation with superovulation traits in Chinese Holstein cows.

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“…Genomic DNA was obtained from blood samples by the method of modified Salting out, described by Miller and coworkers (Miller et al, 1988). To amplify a fragment of 249pb on the INHA exon 1 which contains one reported SNP of A192G (GenBank number: rs41257116) were used the primers F: 5'-GCCCTGTTTCTGGAT-GCC-3' and R: 5'-ATTCAACCCAACCTGCCTA-3' (Tang et al, 2011). The PCR was performed in a final reaction volume of 30 µl, containing 3.8 µl 10X reaction buffer (200 nM (NH 4 ) 2 SO 4, 750 mM Tris-HCl pH 8.8 at 25 °C), 2.5 mM of Mg Cl 2 , 15 pmol of both primers, 0.4 mM of dNTP (deoxyribo nucleotide triphosphate), 1.5 unit of Taq-DNA polymerase (Thermo Scientific) and 100 ng of genomic DNA as template.…”

Section: Dna Extraction and Genotyping By Pcr-rflpmentioning

confidence: 99%

“…In cattle has been reported a change of an adenine for a guanine at position 192 (A192G) on the exon 2 of the INHA, this SNP is considered as a synonymous mutation, this means that the change of an adenine for a guanine does not change the amino acid sequence of the protein, since both codons (CGA and CGG) encode the amino acid Arginine (NCBI, 2013), but result in an additional restriction site for the enzyme MspI. In Chinese Holstein cattle has been found that this SNP is associated with response to superovulatory treatments (Tang et al, 2011) and semen quality (Sang et al, 2011), and the G allele exert a positive effect in both characteristics. However there are no studies about the effect of this polymorphism on the main parameters of zootechnical interest.…”

Section: Introductionmentioning

confidence: 99%

Polimorfismo A192G de INHA y su asociación con características lecheras en ganado Holstein de Antioquia

2015

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El objetivo de esta investigación fue determinar el efecto del polimorfismo A192G en algunos parámetros productivos y reproductivos en vacas Holstein de Antioquia. Para esto, se utilizó la técnica PCR-RFLP para amplificar un segmento de 249 pb del gen de inhibina alfa bovina (INHA) el cual fue digerido con la enzima de restricción MspI. El efecto de los genotipos de INHA en las características productivas y reproductivas se determinó con un Modelo Linear Mixto y la prueba de Tukey se usó para establecer diferencias significativas entre las medias de los tres genotipos. El efecto de sustitución alélica se estudió a través de un modelo de regresión lineal donde los genotipos AA, AG y GG fueron transformados a una escala cuantitativa de 0, 1 y 2, respectivamente de acuerdo al número de alelos G poseídos. En el ganado Holstein de Antioquia el genotipo más común fue el AG (0,529) seguido por el AA (0,269) y el genotipo GG (0,201). Las frecuencias alélicas fueron 0,534 para A y 0.466 para G. Los genotipos de INHA no ejercieron un efecto significativo en los principales parámetros reproductivos, excepto para porcentaje de grasa donde los animales GG presentaron el mayor valor para esta característica. A pesar de que no hubo un efecto significativo de este polimorfismo en los parámetros reproductivos, la prueba de Tukey mostró que el alelo G tiende a tener un efecto negativo en la fertilidad de las hembras, aumentando el intervalo entre partos y el número de servicios por concepción, disminuyendo la eficiencia reproductiva de los hatos. Se requieren más estudios para determinar si estos SNP pueden ser utilizados como marcadores moleculares.

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An MspI polymorphism in the inhibin alpha gene and its associations with superovulation traits in Chinese Holstein cows

Cited by 25 publications

References 24 publications

Multi-Tissue Omics Analyses Reveal Molecular Regulatory Networks for Puberty in Composite Beef Cattle

Multi-Tissue Omics Analyses Reveal Molecular Regulatory Networks for Puberty in Composite Beef Cattle

Polymorphisms of the bovine growth differentiation factor 9 gene associated with superovulation performance in Chinese Holstein cows

Polimorfismo A192G de INHA y su asociación con características lecheras en ganado Holstein de Antioquia

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