An N-nitrosation reaction-based fluorescent probe for detecting nitric oxide in living cells and inflammatory zebrafish

Tang, Qing-Hai; Li, Peijuan; Zhou, Zile; Lu, Qiujun; Gu, Biao; Tang, Siping; Zhang, Youyu

doi:10.1016/j.saa.2021.120728

Cited by 12 publications

(2 citation statements)

References 35 publications

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“…Numerous studies developed NO probes for in vitro and in vivo imaging in different models, which are based mainly on the structure of o-phenylenediamine, Rhodamine 6G, etc. [7][8][9] However, they are not suitable for joint imaging because of the fluorescence intensity in water solution.…”

Section: Introductionmentioning

confidence: 99%

A nitric oxide responsive AIE probe for detecting the progression of osteoarthritis

Jin

Chen

et al. 2023

J. Mater. Chem. B

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Section: Introductionmentioning

confidence: 99%

A nitric oxide responsive AIE probe for detecting the progression of osteoarthritis

Jin

Chen

et al. 2023

J. Mater. Chem. B

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“…However, real-time analysis of NO is challenging due to its unique molecular properties in biological systems, including fast diffusion (diffusion constant 3300 μm/s 2 ), small size, and hydrophobicity (XlogP: 0.2). , Current methods are mainly developed for the indirect analysis of NO, such as high-performance liquid chromatography (HPLC), electron paramagnetic resonance (EPR), chemiluminescence, and fluorescence detection techniques . These methods have been used in many studies but are limited by the complexity of sophisticated instruments and additional chemical reagents.…”

Section: Introductionmentioning

confidence: 99%

Bio-Inspired Electrochemical Detection of Nitric Oxide Promoted by Coordinating the Histamine-Iron Phthalocyanine Catalytic Center on Microelectrode

Guo

Long

et al. 2023

Anal. Chem.

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Biomimetic structures to fabricate bioelectronic interfaces that allow sensors to electrically communicate with electrodes have potential applications in the development of biosensors. Herein, inspired by the structure feature of nitric oxide (NO) sensory protein, we constructed a biomimetically catalytic center, the histamine coordinated iron phthalocyanine (FePc), for efficient and sensitive detection of NO. In specific, NO is recognized by axial tethered FePc, and the oxidative signal of NO on FePc is converted into output signal through electrocatalytic oxidation. Based on the fabricated catalytic structure on the carbon fiber electrode, on one hand, the macrocyclic π system of FePc enabled a rapid redox process, which facilitates electron transfer, thereby greatly improving sensitivity. On the other hand, by coordination with histamine on the electrode surface, FePc can enhance the electrochemical oxidation activity toward NO and promote catalytic detection, which have been revealed by electrochemical characterizations and density functional theory theoretical calculations. The designed electrochemical microsensor exhibits a low limit of detection (0.03 nM) and shows a wide detection range (0.1 nM−2 μM). In addition, the electrochemical microsensor has been successfully used for real-time monitoring of NO release by live cells. So, this work shows a new strategy for the design of bioinspired electrochemical microsensors that may provide a potential analytical tool for tracing biological signal molecules with enzyme-free biomimetically catalytic centers.

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Molecular Imaging of Nitric Oxide Surrogates with Organelle‐Specific Fluorescent Probes

Yadav,

SanuKhan,

Kalita

et al. 2024

Chemistry An Asian Journal

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Nitric oxide is an important signalling molecule responsible for maintaining body’s homeostasis. Any dysregulation in NO can lead to many pathological conditions like atherosclerosis, cancers, neurodegenerative disorders, hypertension, and inflammation. Several, sensing technologies are used for sensing NO. Among these, fluorescent imaging is considered to be one of the most efficient. Till date, approximately 123 fluorescent probes are reported related to nitric oxide (NO) sensing fluorescent probes for the sensitive, selective, and real‐time detection of NO at both the cellular and subcellular levels. In the past five years, around 41 fluorescent probes and four review articles have been published, specifically focusing on the detection of nitric oxide. Despite considerable advancements in this area, no systematic review has summarized various organelle‐targeting NO‐sensing fluorescent probes. Herein, we summarized last five years from 2019 to 2024 along with the key pioneering research in this field covering divergent roles of NO across various cellular organelles. We have included 41 probes by classifying into different organelle targeting sections. We strongly believe this review will provide an advanced summary of NO specific fluorescent probes and their applications for monitoring the progression of diseases in in vitro to in vivo models such as drosophila, zebrafish, mouse models.

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An N-nitrosation reaction-based fluorescent probe for detecting nitric oxide in living cells and inflammatory zebrafish

Cited by 12 publications

References 35 publications

A nitric oxide responsive AIE probe for detecting the progression of osteoarthritis

A nitric oxide responsive AIE probe for detecting the progression of osteoarthritis

Bio-Inspired Electrochemical Detection of Nitric Oxide Promoted by Coordinating the Histamine-Iron Phthalocyanine Catalytic Center on Microelectrode

Molecular Imaging of Nitric Oxide Surrogates with Organelle‐Specific Fluorescent Probes

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