Aiming at the problems of low shortest path selection accuracy, longer response time, and poor selection effect in current cold chain logistics transportation methods, a cold chain logistics transportation shortest path selection algorithm based on improved artificial bee colony is proposed. The improved algorithm is used to initialize the food source, reevaluate the fitness value of the food source, generate a new food source, optimize the objective function and food source evaluation strategy, and get an improved artificial bee colony algorithm. Based on the improved artificial bee colony algorithm, the group adaptive mechanism of particle swarm algorithm is introduced to initialize the position and velocity of each particle randomly. Dynamic detection factor and octree algorithm are adopted to dynamically update the path of modeling environment information. According to the information sharing mechanism between individual particles, the group adaptive behavior control is performed. After the maximum number of cycles, the path planning is completed, the shortest path is output, and the shortest path selection of cold chain logistics transportation is realized. The experimental results show that the shortest path selection effect of the cold chain logistics transportation of the proposed algorithm is better, which can effectively improve the shortest path selection accuracy and reduce the shortest path selection time.