An odorant receptor that senses four classes of musk compounds

Yoshikawa, Keiichi; Deguchi, Jun; Jieying, Hu; Lu, Hsiu-Yi; Matsunami, Hiroaki

doi:10.1016/j.cub.2022.10.038

Cited by 7 publications

(3 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Vapor-phase stimulation was observed as indicated by the finding that no response was observed when a non-volatile cellular stimulant (i.e., forskolin) was tested. Activations of a consensus version of OR5A2 (hereafter, cOR5A2) and OR9Q2 against the vapor phase of Amb and p -cresol were monitored 55 . The result showed that a low volume of medium covering OR-expressing cells caused a lower maximum response; a 60% reduction in the amount of medium (from 50 to 20 μL) decreased the response amplitude of cOR5A2 and OR9Q2 to 33% and 35%, respectively (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Dual-Glo™ luciferase assay (Promega, Madison, WI, USA) was performed as previously described 55 . Briefly, FLAG-Rho-tagged OR, cAMP response element (CRE)/luc2PpGL4.29, pRL-CMV, and RTP1S were added to Dulbecco’s Modified Eagle Medium (DMEM) with polyethylenimine Max (PEI-MAX, Polysciences, Warrington, PA, USA) for each well of a poly- d -lysine-coated 96-well plate (Corning) or poly- d -lysine-coated 384-well plate (Corning).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Functions of human olfactory mucus and age-dependent changes

Shirai

Takase

Yokoyama

et al. 2023

Sci Rep

Self Cite

View full text Add to dashboard Cite

Odorants are detected by olfactory sensory neurons, which are covered by olfactory mucus. Despite the existence of studies on olfactory mucus, its constituents, functions, and interindividual variability remain poorly understood. Here, we describe a human study that combined the collection of olfactory mucus and olfactory psychophysical tests. Our analyses revealed that olfactory mucus contains high concentrations of solutes, such as total proteins, inorganic elements, and molecules for xenobiotic metabolism. The high concentrations result in a capacity to capture or metabolize a specific repertoire of odorants. We provide evidence that odorant metabolism modifies our sense of smell. Finally, the amount of olfactory mucus decreases in an age-dependent manner. A follow-up experiment recapitulated the importance of the amount of mucus in the sensitive detection of odorants by their receptors. These findings provide a comprehensive picture of the molecular processes in olfactory mucus and propose a potential cause of olfactory decline.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Functions of human olfactory mucus and age-dependent changes

Shirai

Takase

Yokoyama

et al. 2023

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…[25] Yet, it still remains unclear how odorants are recognized, and by how much energy an odorant receptor can induce and stabilize conformations. The human olfactory receptor OR5 A2 is now generally considered to be the prime musk receptor, [26] reportedly activated by all the structurally diverse families of musks; [27] yet its binding cavity is rather large and unspecific, and thus it would be going too far to rationalize why the compounds reported do not bind. Rather the powdery, creamy, fruity ionone odor of 49 can be explained.…”

Section: Discussionmentioning

confidence: 99%

Synthesis and Olfactory Properties of Lower‐Ring Homologues of the Linear Alicyclic Musk Romandolide

Dacho,

Nikipelov,

Tóthová

et al. 2023

Helvetica Chimica Acta

View full text Add to dashboard Cite

In order to expand the knowledge on linear alicyclic musks, a set of 29 small‐ring (racemic) analogues of the biodegradable and renewable alicyclic musk Romandolide was designed, prepared, and evaluated. The common short and modular synthesis employs either commercially available or easily accessible (substituted) cyclopropyl/cyclobutyl ketones and acids. These were transformed in three/four steps to the target compounds. Their qualitative olfactory analysis reveals that contraction of cyclohexane ring of Romandolide to smaller rings annihilates the genuine musk scent, though many of these share the metallic hot‐iron off‐note of some macrocyclic musks. Indeed, these new derivatives exhibit a plethora of pleasant, interesting, and potentially useful scents including herbal, green, fruity, or chocolate ones accompanied by various undertones. The powdery, fruity ionone odor of the dimethyl cyclobutyl compound was found to be most interesting as it possesses a very natural raspberry and violet character. Computational modelling suggest that the lowest‐energy conformers of the target compounds do either not adopt a true U‐shape as was speculated to be a prerequisite for a musk odor, or that the quaternary carbon atom of the dimethyl‐substituted cyclobutane is not well placed to fit into a hydrophobic binding pocket on the corresponding receptor site.

show abstract

Molecular determinants of olfactory receptor activation: Comparative analysis of Olfr205 and Olfr740 family member responses to indole

Li,

Wang,

et al. 2024

Archives of Biochemistry and Biophysics

View full text Add to dashboard Cite

An odorant receptor that senses four classes of musk compounds

Cited by 7 publications

References 34 publications

Functions of human olfactory mucus and age-dependent changes

Functions of human olfactory mucus and age-dependent changes

Synthesis and Olfactory Properties of Lower‐Ring Homologues of the Linear Alicyclic Musk Romandolide

Molecular determinants of olfactory receptor activation: Comparative analysis of Olfr205 and Olfr740 family member responses to indole

Contact Info

Product

Resources

About