An open-source platform for head-fixed operant and consummatory behavior

Gordon-Fennell, Adam; Barbakh, Joumana M; Utley, MacKenzie T; Bazzino, Paula; Gowrishankar, Raaj; Bruchas, Michael R.; Roitman, Mitchell F.; Stuber, Garret D.

doi:10.7554/elife.86183

Cited by 12 publications

(3 citation statements)

References 83 publications

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“…For the 4 days prior to testing, animals were habituated to handling, fiber photometry cable attachment, and head-fixation. Animals were head-fixed to minimize motion-related artifacts in the fiber photometry signal, and all head-fixed testing was completed using the open-source OHRBETS platform 77 .…”

Section: Methodsmentioning

confidence: 99%

Development of a genetically encoded sensor for probing endogenous nociceptin opioid peptide release

Zhou,

Stine,

Prada

et al. 2024

Nat Commun

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Nociceptin/orphanin-FQ (N/OFQ) is a recently appreciated critical opioid peptide with key regulatory functions in several central behavioral processes including motivation, stress, feeding, and sleep. The functional relevance of N/OFQ action in the mammalian brain remains unclear due to a lack of high-resolution approaches to detect this neuropeptide with appropriate spatial and temporal resolution. Here we develop and characterize NOPLight, a genetically encoded sensor that sensitively reports changes in endogenous N/OFQ release. We characterized the affinity, pharmacological profile, spectral properties, kinetics, ligand selectivity, and potential interaction with intracellular signal transducers of NOPLight in vitro. Its functionality was established in acute brain slices by exogeneous N/OFQ application and chemogenetic induction of endogenous N/OFQ release from PNOC neurons. In vivo studies with fibre photometry enabled direct recording of NOPLight binding to exogenous N/OFQ receptor ligands, as well as detection of endogenous N/OFQ release within the paranigral ventral tegmental area (pnVTA) during natural behaviors and chemogenetic activation of PNOC neurons. In summary, we show here that NOPLight can be used to detect N/OFQ opioid peptide signal dynamics in tissue and freely behaving animals.

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Section: Methodsmentioning

confidence: 99%

Development of a genetically encoded sensor for probing endogenous nociceptin opioid peptide release

Zhou,

Stine,

Prada

et al. 2024

Nat Commun

Self Cite

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show abstract

“…Animals were head-fixed to minimize motion-related artefacts in the fibre photometry signal, and all head-fixed testing was completed using the open-source OHRBETS platform. 78…”

Section: Methodsmentioning

confidence: 99%

Development of a genetically encoded sensor for probing endogenous nociceptin opioid peptide release

Zhou

Stine

Prada

et al. 2023

Preprint

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Nociceptin/orphanin-FQ (N/OFQ) is a recently appreciated critical opioid peptide with key regulatory functions in several central behavioral processes including motivation, stress, feeding, and sleep. The functional relevance of N/OFQ action in the mammalian brain remains unclear due to a lack of high-resolution approaches to detect this neuropeptide with appropriate spatial and temporal resolution. Here we develop and characterize NOPLight, a genetically encoded sensor that sensitively reports changes in endogenous N/OFQ release. We characterized the affinity, pharmacological profile, spectral properties, kinetics, ligand selectivity, and potential interaction with intracellular signal transducers of NOPLight in vitro. Its functionality was established in acute brain slices by exogeneous N/OFQ application and chemogenetic induction of endogenous N/OFQ release from PNOC neurons. In vivo studies with fiber photometry enabled a direct recording of binding by N/OFQ receptor ligands, as well as the detection of natural or chemogenetically-evoked endogenous N/OFQ release within the paranigral ventral tegmental area (pnVTA). In summary, we show that NOPLight can be used to detect N/OFQ opioid peptide signal dynamics in tissue and freely-behaving animals.

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“…Now, in eLife, Garret Stuber and colleagues from the University of Washington and University of Illinois at Chicago – including Adam Gordon-Fennell as first author – report a new low-cost system for conducting behavioral experiments related to motivation on head-fixed mice ( Gordon-Fennell et al, 2023 ). The tool – which is named OHRBETS (short for Open-Source Head-fixed Rodent Behavioral Experimental Training System) – consists of two configurations ( Figure 1 ).…”

mentioning

confidence: 99%