An Optical Approach for Drug Screening Based on Light‐Harvesting Conjugated Polyelectrolytes

An, Lingling; Liu, Libing; Wang, Shu; Bazan, Guillermo C.

doi:10.1002/anie.200900758

Cited by 20 publications

(13 citation statements)

References 53 publications

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“…In addition to applications tailored for highly sensitive biomacromolecular detection, recent approaches have made efforts to develop water‐soluble CPEs as a new class of optical platform for the screening of small‐molecule active drugs against biological targets such as nucleic acids, enzymes, and RNA–protein complexes . CPEs have been used in fluorescence assays for real‐time screening of small molecules that prevent the RNA–protein complexation critical to virus replication and thereby can be considered potential initial candidates for drug discovery .…”

Section: Resultsmentioning

confidence: 99%

Cell imaging and DNA delivery in fibroblastic cells by conjugated polyelectrolytes

Shin

Lee

Jeong

et al. 2013

Biotech and App Biochem

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This study concentrates on the potential application of conjugated polyelectrolytes (CPEs) to cell imaging and DNA delivery. Four different types of polyfluorene copolymers, namely, PAHFP-Br, PAEFP-Br, PAHFbT-Br, and PSBFP-Na, which have the same π-conjugated backbone but different side chains, were synthesized. For cytotoxicity testing, L-929 fibroblastic cells were treated with increasing concentrations (0-50 µM) of each CPE and then cell viability was determined by WST-8 assay. Cellular uptake of CPEs into cultured L-929 cells was observed by fluorescence microscopy. To examine DNA delivery by CPEs, the cells were incubated for 1 H with PAHFP-Br/fluorescein (Fl)-labeled single-stranded DNA (ssDNA-Fl) complex and then visualized by fluorescence microscopy. Cytotoxicity of CPEs was increased in a dose-dependent manner but at lower than 10 µM, PAHFP-Br, PAEFP-Br, and PSBFP-Na did not show any cytotoxic effects on the cells. When added to cell cultures at 1 µM, PAHFP-Br/ssDNA-Fl complex was delivered and then dissociated into PAHFP-Br and ssDNA-Fl within the cells. This result implies that PAHFP-Br can enable cell imaging and DNA delivery into fibroblastic cells. Therefore, it is suggested that PAHFP-Br with various advantages such as low cytotoxicity and high fluorescence efficiency can be extensively used as a potential agent for cell imaging and gene delivery.

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Section: Resultsmentioning

confidence: 99%

Cell imaging and DNA delivery in fibroblastic cells by conjugated polyelectrolytes

Shin

Lee

Jeong

et al. 2013

Biotech and App Biochem

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“…Light‐harvesting antenna materials have attracted great interest owing to their applications for sensors, photocatalysis, photosynthesis, and optoelectronic devices . This kind of systems absorbs solar light and transfers the excitation energy to a reaction centre by highly efficient energy transfer.…”

Section: Introductionmentioning

confidence: 99%

Artificial Light‐Harvesting Material Based on Self‐Assembly of Coordination Polymer Nanoparticles

et al. 2014

Adv Funct Materials

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Artifi cial light-harvesting antenna materials as potential mimics for photosynthetic systems have attracted intense attention recently. Herein, a new modular approach to construct light-harvesting material, which involves the self-assembly of coordination polymer nanoparticles (CPNs) at room temperature, is presented. Fluorescence resonance energy transfer (FRET) occurs between donor and acceptor molecules encapsulated in the CPNs, and the emission signal of acceptor is amplifi ed signifi cantly. To the best of our knowledge, this is the fi rst example of artifi cial light-harvesting material generated from biomolecule-based coordination polymer nanoparticles. The modularity of the material makes it convenient to manipulate the system by changing the composite of CPNs and the type and amount of dyes confi ned, implying it is a general strategy. The material functions not only in fl uid medium, but also in the form of solid state, which extends its application areas greatly. Furthermore, photocurrent generation can be realized by the dye-encapsulated CPNs system upon irradiation with visible light, implying the potential usefulness in light-energy conversion and photoelectronic applications. Besides, the creation of FRET system provides a platform to mimic dual-channel logic gate at nanoscale level, which is benefi cial to the construction of integrated logic devices with multiple functions.

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“…[24][25][26] Due to the highly electron-delocalized backbones inherited from their neutral counterpart-oil-soluble conjugated polymers, 27,28 CPEs have a distinguished capability to harvest and emit light energy, manifesting superior optoelectronic properties compared to conventional small organic uorophores, such as high optical absorption cross-section, good photo-stability, and large Stokes shi. 25,29,30 Simultaneously, the electrostatic-mediated physicochemical behaviors in aqueous media derived from polyelectrolytes allow CPEs to interact with various biological substances, such as DNA, RNA and protease. 31,32 Integration of the above two features gives rise to extensive biological applications of CPEs, including biological sensors 30,[33][34][35] and uorescence imaging agents for cells and tissues.…”

Section: Introductionmentioning

confidence: 99%

“…25,29,30 Simultaneously, the electrostatic-mediated physicochemical behaviors in aqueous media derived from polyelectrolytes allow CPEs to interact with various biological substances, such as DNA, RNA and protease. 31,32 Integration of the above two features gives rise to extensive biological applications of CPEs, including biological sensors 30,[33][34][35] and uorescence imaging agents for cells and tissues. [36][37][38] Molecular structures of the cationic CPE (TBtPFN) and anionic CPE (BtPFS) are shown in Scheme 1.…”

Section: Introductionmentioning

confidence: 99%

A fluorescence–Raman dual-imaging platform based on complexes of conjugated polymers and carbon nanotubes

et al. 2014

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The present study describes a flexible nanoplatform based on electrostatic assembly of conjugated polyelectrolytes (CPEs) and carboxylated multi-walled carbon nanotubes (cMWNTs). It is demonstrated that the obtained nanocomposites inherit intrinsic optical properties of CPEs and characteristic Raman vibration modes of MWNTs, providing a fluorescence-Raman dual-imaging method for intracellular tracking and locating of MWNTs. We suggest that the cellular internalization of the CPE-cMWNT nanocomposites is a surface charge-dependent process. The strengths of this nanoplatform include satisfying biocompatibility, enhanced protein-repellent property, and ease of implementation, making it available for both in vitro and in vivo applications.

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An Optical Approach for Drug Screening Based on Light‐Harvesting Conjugated Polyelectrolytes

Cited by 20 publications

References 53 publications

Cell imaging and DNA delivery in fibroblastic cells by conjugated polyelectrolytes

Cell imaging and DNA delivery in fibroblastic cells by conjugated polyelectrolytes

Artificial Light‐Harvesting Material Based on Self‐Assembly of Coordination Polymer Nanoparticles

A fluorescence–Raman dual-imaging platform based on complexes of conjugated polymers and carbon nanotubes

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