An optical microplate biosensor for the detection of methyl parathion pesticide using a biohybrid of Sphingomonas sp. cells-silica nanoparticles

Mishra, Archana; Kumar, Jitendra; Melo, Jose Savio

doi:10.1016/j.bios.2016.08.048

Cited by 77 publications

(26 citation statements)

References 34 publications

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“…Despite these applications, however, the potential risks of SNPs against human heath have not been fully assessed. Recently, SNPs have been widely used for the targeted delivery of contrast agents and drugs and biomedical applications such as biosensors, microscopic imaging, DNA delivery, and enzyme immobilization, in order to improve disease diagnosis and therapy 5,6. The sufficiently small size of SNPs, like that of other nanoparticles, can penetrate relatively large pores of blood vessels around diseased regions, such as in cancer 7.…”

Section: Introductionmentioning

confidence: 99%

<p>A reliable approach for assessing size-dependent effects of silica nanoparticles on cellular internalization behavior and cytotoxic mechanisms</p>

Kim¹,

Kim²,

Lee³

et al. 2019

IJN

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BackgroundThe size of nanoparticles is considered to influence their toxicity, as smaller-sized nanoparticles should more easily penetrate the cell and exert toxic effects. However, conflicting results and unstandardized methodology have resulted in controversy of these size-dependent effects. Here, we introduce a unique approach to study such size-dependent effects of nanoparticles and present evidence that reliably supports this general assumption along with elucidation of the underlying cytotoxic mechanism.MethodsWe prepared and physically characterized size-controlled (20–50 nm) monodispersed silica nanoparticles (SNPs) in aqueous suspensions. Then, a variety of biochemical assessments are used for evaluating the cytotoxic mechanisms.ResultsSNP treatment in three cell lines decreased cell viability and migration ability, while ROS production increased in dose- and size-dependent manners, with SNPs <30 nm showing the greatest effects. 30- and 40-nm SNPs were observed similar to these biological activities of 20- and 50-nm, respectively. Under the conventionally used serum-free conditions, both 20-nm and 50-nm SNPs at the IC50 values (75.2 and 175.2 μg/mL) induced apoptosis and necrosis in HepG2 cells, whereas necrosis was more rapid with the smaller SNPs. Inhibiting endocytosis impeded the internalization of the 50-nm but not the 20-nm SNPs. However, agglomeration following serum exposure increased the size of the 20-nm SNPs to approximately 50 nm, preventing their internalization and cell membrane damage without necrosis. Thus, 20-nm and 50-nm SNPs show different modes of cellular uptake, with smaller SNPs capable of trafficking into the cells in an endocytosis-independent manner. This approach of using non-overlapping size classes of SNPs under the same dose, along with serum-induced agglomeration analysis clarifies this long-standing question about the safety of small SNPs.ConclusionOur results highlight the need to revise safety guidelines to account for this demonstrated size-dependent cytotoxicity under serum-free conditions, which may be similar to the microenvironment after tissue penetration.

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Section: Introductionmentioning

confidence: 99%

<p>A reliable approach for assessing size-dependent effects of silica nanoparticles on cellular internalization behavior and cytotoxic mechanisms</p>

Kim¹,

Kim²,

Lee³

et al. 2019

IJN

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“…Then, a magnetic field can be used to form a sandwich-like complex of the two probes with a test sample containing a target protein (e.g., sera, pathogen culture, body fluid) to form “magnetic microsphere-target protein-AuNP”. After dissociation of the labelled DNA barcode strands on the gold nanoprobes via de-hybridization elution release, the target protein content can be determined by the selected colorimetric, fluorescence labelling, biochip or other detection method [18] , [19] , [20] , [21] , [22] , [23] . The main schematic diagram is shown in Fig.…”

Section: The Principle Of Bio-barcode Technology and A Methods Applicamentioning

confidence: 99%

Bio-barcode detection technology and its research applications: A review

Wang

Jin

Chen

et al. 2019

Journal of Advanced Research

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“…A whole-cell optical biosensor based on Sphingomonas sp. cells was recently proposed as an interesting alternative for methyl parathion detection [ 17 ]. The optical biosensor used silica nanoparticles functionalized with polyethyleneimine, which provided an enhanced storage stability (180 days with a retention of 85% of activity when stored at 4 °C), mainly due to the protective covering provided around the cells also reducing the leaching of the hydrolytic enzyme.…”

Section: Biosensors For Environmental Monitoringmentioning

confidence: 99%

“…The optical biosensor used silica nanoparticles functionalized with polyethyleneimine, which provided an enhanced storage stability (180 days with a retention of 85% of activity when stored at 4 °C), mainly due to the protective covering provided around the cells also reducing the leaching of the hydrolytic enzyme. This excellent storage stability enables many routine applications such as decentralized field testing, screening, and rapid detection of multiple samples, avoiding the time-consuming preparation of a fresh biosensor [ 17 ]. In addition, the optical biosensor is reusable, since, after ten repeated reactions, 81% of the enzyme activity was maintained.…”

Section: Biosensors For Environmental Monitoringmentioning

confidence: 99%

Recent Progress in Biosensors for Environmental Monitoring: A Review

Justino

Duarte

Rocha-Santos

2017

Sensors

304

125

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The environmental monitoring has been one of the priorities at the European and global scale due to the close relationship between the environmental pollution and the human health/socioeconomic development. In this field, the biosensors have been widely employed as cost-effective, fast, in situ, and real-time analytical techniques. The need of portable, rapid, and smart biosensing devices explains the recent development of biosensors with new transduction materials, obtained from nanotechnology, and for multiplexed pollutant detection, involving multidisciplinary experts. This review article provides an update on recent progress in biosensors for the monitoring of air, water, and soil pollutants in real conditions such as pesticides, potentially toxic elements, and small organic molecules including toxins and endocrine disrupting chemicals.

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An optical microplate biosensor for the detection of methyl parathion pesticide using a biohybrid of Sphingomonas sp. cells-silica nanoparticles

Cited by 77 publications

References 34 publications

<p>A reliable approach for assessing size-dependent effects of silica nanoparticles on cellular internalization behavior and cytotoxic mechanisms</p>

<p>A reliable approach for assessing size-dependent effects of silica nanoparticles on cellular internalization behavior and cytotoxic mechanisms</p>

Bio-barcode detection technology and its research applications: A review

Recent Progress in Biosensors for Environmental Monitoring: A Review

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