An optical oxygen sensor and reaction vessel for high‐pressure applications

Stokes, M. Dale; Somero, George N.

doi:10.4319/lo.1999.44.1.0189

Cited by 21 publications

(13 citation statements)

References 33 publications

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“…The active chemical compound used on this sensor is a platinum porphyrine complex, which yields a longer and more easily detectable lifetime than the more commonly used ruthenium complexes (e.g., Klimant et al, 1996;Stokes and Romero, 1999). Ruthenium-based sensors will likely perform similarly to the sensors tested here, but their accuracy and precision are expected to be lower, due to the shorter lifetime of ruthenium.…”

Section: Discussion Comments and Recommendationsmentioning

confidence: 99%

“…The most commonly used oxygen lumniphores have been ruthenium complexes (e.g., Klimant et al, 1996;Stokes and Romero, 1999), but for this sensor an oxygen-sensitive luminophore based on a platinum porphyrine complex, commercially available from PreSens GmbH (Regensburg, Germany), was used mainly because of its longer lifetime (about a factor of 5 longer than ruthenium's). A longer lifetime makes it easier to detect the signal and to obtain stable readings.…”

Section: Materials and Proceduresmentioning

confidence: 99%

“…The different signal detection techniques are summarized by Wolfbeis (1991), Demas et al (1999), and Glud et al (2000) along with a wide range of applications. Klimant et al (1995) and Stokes and Romero (1999) described intensity-based oxygen optodes and their use. The function and use of lifetimebased optodes were described by Holst et al (1995) and Klimant et al (1996).…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of a lifetime‐based optode to measure oxygen in aquatic systems

Tengberg

Hovdenes

Andersson

et al. 2006

Limnology & Ocean Methods

223

199

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In this article, we evaluate the performance of a commercially available lifetime-based optode and compare it with data obtained by other methods. We performed a set of 10 different tests, including targeted laboratory evaluations and field studies, covering a wide range of situations from shallow coastal waters and wastewater treatment plants to abyssal depths. Our principal conclusion is that, owing to high accuracy (± 2 µM), long-term stability (more than 20 months), lack of pressure hysteresis, and limited cross-sensitivity, this method is overall more suitable for oxygen monitoring than other methods.

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Section: Discussion Comments and Recommendationsmentioning

confidence: 99%

Section: Materials and Proceduresmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evaluation of a lifetime‐based optode to measure oxygen in aquatic systems

Tengberg

Hovdenes

Andersson

et al. 2006

Limnology & Ocean Methods

223

199

View full text Add to dashboard Cite

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“…The optode uses the effect of dynamic luminescence quenching by molecular oxygen for measuring dissolved oxygen in salt water. Optodes have the advantage that their pressure response is predictable (Glud et al 2001;Stokes and Somero 1999). The oxygen optode was set to sample oxygen concentration and temperature every 30 s and record the data to the laboratory computer.…”

Section: Materials and Proceduresmentioning

confidence: 99%

Development of a hyperbaric trap-respirometer for the capture and maintenance of live deep-sea organisms

Drazen

Bird

Barry

2005

Limnol. Oceanogr. Methods

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A major obstacle to the investigation of deep-sea biology is the lack of instrumentation to retrieve deep-sea organisms from their habitat alive, particularly fishes with physoclistous swimbladders. To perform physiological experiments on deep-sea fishes under in situ but controlled conditions, we constructed a high-pressure fish trap-respirometer to capture deep-water fishes at depth and return them to the surface alive at in situ pressure and temperature. Pumps and instrumentation connected aboard ship or in the laboratory are used for maintenance of the animal and experimentation. The trap was designed so that respiration rates, pressure tolerance, and metabolic responses to various gas concentrations (CO 2 and O 2 ) could be examined in a controlled environment. The trap is deployed as an autonomous lander or free vehicle to depths of 4000 m. Once on the seafloor, a fish is captured on a baited hook that triggers the reeling of the fish into the pressure vessel and closure of its sealing door. Two fish, Coryphaenoides acrolepis, have been recovered live from 1450 m and maintained in the laboratory. Both fish were retrieved at ~95% of their in situ pressure and at temperatures of ~6°C. Oxygen consumption rates of these fish were 54.99 µmol O 2 kg -1 h -1 (1.158 kg, 65.0 cm total length, 23.5 cm pre-anal fin length) and 79.43 µmol O 2 kg -1 h -1 (1.305 kg, 66.5 cm total length, 24.5 cm pre-anal fin length). The latter fish was maintained for 3.5 d, and it survived gradual decompression to 76% of the pressure at its capture depth.

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“…The intensity of the fluorescence emitted depends on the oxygen concentration. This technique has been applied to sediment samples (Klimant et al, 1995), microbial cultures under high pressure (Stokes and Somero, 1999), and bioreactors (Voraberger et al, 2001). Commercial models are now available from several companies, such as the Oxygen Optode (Aanderaa Instruments, Norway), FOXY Fiber Optic Oxygen Sensor (Ocean Optics, USA), and FOX 5000 series (ASR, Japan).…”

mentioning

confidence: 99%