An optical PMMA biochip based on fluorescence anisotropy: Application to C-reactive protein assay

Baldini, Francesco; Carloni, Adolfo; Giannetti, Ambra; Porro, G. Bianchi; Trono, C.

doi:10.1016/j.snb.2008.08.027

Cited by 50 publications

(27 citation statements)

References 14 publications

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“…PMMA surfaces can immobilize proteins such as streptavidin [41], single-chain variable fragments of antibodies [42], and C-reactive proteins [43] and minutes. Moreover, a much higher noise level was observed for the QCM-P device, which may due to a short-term stability of the physical adsorption [46].…”

Section: Application Of Qcm-p For Acrylic Acid Graftingmentioning

confidence: 99%

Numerical simulation and experimental study of resonance characteristics of QCM-P devices operating in liquid and their application in biological detection

Wang

Gong

et al. 2015

Sensors and Actuators B: Chemical

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Section: Application Of Qcm-p For Acrylic Acid Graftingmentioning

confidence: 99%

Numerical simulation and experimental study of resonance characteristics of QCM-P devices operating in liquid and their application in biological detection

Wang

Gong

et al. 2015

Sensors and Actuators B: Chemical

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“…Using this setup of thin film waveguides and grating couplers, a limit of detection as low as 50 fM could be reached. Baldini and coworkers 7,8 employed an inverse setup with respect to the two abovementioned optical systems. Here the fluorescence spots were directly illuminated with excitation light perpendicular to the surface and the polymer substrate was exploited as waveguide to collect the emitted fluorescence.…”

Section: Tirf Based Optical Detectionmentioning

confidence: 99%

Optical biosensor system with integrated microfluidic sample preparation and TIRF based detection

Gilli

Scheicher

Suppan

et al. 2013

Optical Sensors 2013

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There is a steadily growing demand for miniaturized bioanalytical devices allowing for on-site or point-of-care detection of biomolecules or pathogens in applications like diagnostics, food testing, or environmental monitoring. These, so called labs-on-a-chip or micro-total analysis systems (µ-TAS) should ideally enable convenient sample-in -result-out type operation. Therefore, the entire process from sample preparation, metering, reagent incubation, etc. to detection should be performed on a single disposable device (on-chip). In the early days such devices were mainly fabricated using glass or silicon substrates and adapting established fabrication technologies from the electronics and semiconductor industry. More recently, the development focuses on the use of thermoplastic polymers as they allow for low-cost high volume fabrication of disposables. One of the most promising materials for the development of plastic based lab-on-achip systems are cyclic olefin polymers and copolymers (COP/COC) due to their excellent optical properties (high transparency and low autofluorescence) and ease of processing.We present a bioanalytical system for whole blood samples comprising a disposable plastic chip based on TIRF (total internal reflection fluorescence) optical detection. The chips were fabricated by compression moulding of COP and microfluidic channels were structured by hot embossing. These microfluidic structures integrate several sample pretreatment steps. These are the separation of erythrocytes, metering of sample volume using passive valves, and reagent incubation for competitive bioassays. The surface of the following optical detection zone is functionalized with specific capture probes in an array format. The plastic chips comprise dedicated structures for simple and effective coupling of excitation light from low-cost laser diodes. This enables TIRF excitation of fluorescently labeled probes selectively bound to detection spots at the microchannel surface. The fluorescence of these detection arrays is imaged using a simple set-up based on a digital consumer camera. Image processing for spot detection and intensity calculation is accomplished using customized software. Using this combined TIRF excitation and imaging based detection approach allowes for effective suppression of background fluorescence from the sample, multiplexed detection in an array format, as well as internal calibration and background correction.

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“…[17][18][19] Polymers, such as polymethylmethacrylate (PMMA), are highly transparent and have very low autofluorescence over a wide spectral range, 20 making it a well used material in microfluidics. The PMMA can be patterned in all the above ways; however, hot embossing and injection molding are high-volume methods and have high-tooling costs, making their applicability to a research environment low.…”

Section: Introductionmentioning

confidence: 99%

Laser micromachined wax-covered plastic paper as both sputter deposition shadow masks and deep-ultraviolet patterning masks for polymethylmethacrylate-based microfluidic systems

Fan

et al. 2013

J. Micro/Nanolith. MEMS MOEMS

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Abstract. We report a technically innovative method of fabricating masks for both deep-ultraviolet (UV) patterning and metal sputtering on polymethylmethacrylate (PMMA) for microfluidic systems. We used a CO 2 laser system to cut the required patterns on wax-covered plastic paper; the laser-patterned wax paper will either work as a mask for deep-UV patterning or as a mask for metal sputtering. A microfluidic device was also fabricated to demonstrate the feasibility of this method. The device has two layers: the first layer is a 1-mm thick PMMA substrate that was patterned by deep-UV exposure to create microchannels. The mask used in this process was the laser-cut wax paper. The second layer, also a 1-mm thick PMMA layer, was gold sputtered with patterned wax paper as the shadow mask. These two pieces of PMMA were then bonded to form microchannels with exposed electrodes. This process is a simple and rapid method for creating integrated microfluidic systems that do not require cleanroom facilities.

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An optical PMMA biochip based on fluorescence anisotropy: Application to C-reactive protein assay

Cited by 50 publications

References 14 publications

Numerical simulation and experimental study of resonance characteristics of QCM-P devices operating in liquid and their application in biological detection

Numerical simulation and experimental study of resonance characteristics of QCM-P devices operating in liquid and their application in biological detection

Optical biosensor system with integrated microfluidic sample preparation and TIRF based detection

Laser micromachined wax-covered plastic paper as both sputter deposition shadow masks and deep-ultraviolet patterning masks for polymethylmethacrylate-based microfluidic systems

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