An optimal manual procedure for ammonia analysis in natural waters by the indophenol blue method

Ivančić, Ingrid; Degobbis, Danilo

doi:10.1016/0043-1354(84)90230-6

Cited by 355 publications

(166 citation statements)

References 12 publications

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“…The concentrations of dissolved silicate, nitrate and nitrite were also estimated daily, according to Strickland and Parsons (1972). The concentration of dissolved ammonium was assessed according to Ivančič and Deggobis (1984). The detection limits for phosphate, nitrate and ammonium were 0.8 nM, 0.017 and 0.019 µM, respectively.…”

Section: Dissolved Nutrients and Dissolved Organic Carbonmentioning

confidence: 99%

Bacterial Growth and Mortality after Deposition of Saharan Dust and Mixed Aerosols in the Eastern Mediterranean Sea: A Mesocosm Experiment

et al. 2017

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The impact of viral lysis and grazing by flagellates on bacterioplankton production was assessed during a mesocosm experiment in the Eastern Mediterranean Sea, in response to Saharan dust (SD) vs. mixed aerosols (A) addition. The results highlight a positive effect on bacterial abundance, production and growth rate (∼1.2, ∼2.4, and ∼1.9 -fold higher than the controls) in both SD and A, which was also confirmed by the increased portion of high DNA content bacteria (up to 48% of the bacterial community). Lytic viral production and the portion of bacterial production lost due to viral lysis were lower in SD and A after dust addition than in the controls (0.33 ± 0.17 × 10 6 virus-like particles mL −1 h −1 and 6 ± 4%, respectively). Potential ingestion rate of bacteria by flagellates increased upon dust enrichment, but did not differ between mesocosms. Larger predators possibly down regulated flagellate abundance, and the calculated portion of bacterial production lost due to flagellate grazing was probably an artifact. Higher frequency of lysogenic cells in A compared to SD and the controls four days after dust addition may reflect faster phosphorus limitation in A, due to receiving less dissolved inorganic phosphorus and more dissolved inorganic nitrogen than SD.

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Section: Dissolved Nutrients and Dissolved Organic Carbonmentioning

confidence: 99%

Bacterial Growth and Mortality after Deposition of Saharan Dust and Mixed Aerosols in the Eastern Mediterranean Sea: A Mesocosm Experiment

et al. 2017

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“…Experimental values were subtracted from blank values and the resulting computed O 2 consumption rates are reported in µmol g −1 h −1 (wet mass). A second sample of water was immediately drawn and frozen in a cryovial at −80 • C. These samples were later thawed and the NH 3 concentration (µmol g −1 h −1 wet mass) was measured using the indophenol blue colorimetric assay (Ivancic and Degobbis, 1984). Organisms were weighed using a motion-compensated shipboard balance system (Childress and Mickel, 1980), then frozen in liquid nitrogen.…”

Section: Methodsmentioning

confidence: 99%

The metabolic response of pteropods to acidification reflects natural CO<sub>2</sub>-exposure in oxygen minimum zones

2012

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Abstract. Shelled pteropods (Thecosomata) are a group of holoplanktonic mollusks that are believed to be especially sensitive to ocean acidification because their aragonitic shells are highly soluble. Despite this concern, there is very little known about the physiological response of these animals to conditions of elevated carbon dioxide. This study examines the oxygen consumption and ammonia excretion of five pteropod species, collected from tropical regions of the Pacific Ocean, to elevated levels of carbon dioxide (0.10 %, 1000 ppm). Our results show that pteropods that naturally migrate into oxygen minimum zones, such as Hyalocylis striata, Clio pyramidata, Cavolinia longirostris and Creseis virgula, were not affected by carbon dioxide at the levels and duration tested. Diacria quadridentata, which does not migrate, responds to high carbon dioxide conditions with reduced oxygen consumption and ammonia excretion. This indicates that the natural chemical environment of individual species may influence their resilience to ocean acidification.

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“…Inorganic Nutrients, Particulate Organic C and N Water samples were analyzed daily for phosphate according to the MAGIC method (Rimmelin and Moutin, 2005), for silicate, nitrite, and nitrate according to Strickland and Parsons (1972), and for ammonium according to Ivancic and Degobbis (1984). The detection limits were 0.8 nM for phosphate, 0.017 µM for nitrate, 0.019 µM for ammonium, and 0.025 µM for silicate.…”

Section: Total Dissolved Phosphorus (Tdp)mentioning

confidence: 99%

Saharan Dust Deposition Effects on the Microbial Food Web in the Eastern Mediterranean: A Study Based on a Mesocosm Experiment

et al. 2017

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The effect of episodicity of Saharan dust deposition on the pelagic microbial food web was studied in the oligotrophic Eastern Mediterranean by means of a mesocosm experiment in May 2014. Two different treatments in triplicates (addition of natural Saharan dust in a single-strong pulse or in three smaller consecutive doses of the same total quantity), and three unamended controls were employed; chemical and biological parameters were measured during a 10-day experiment. Temporal changes in primary (PP) and bacterial (BP) production, chlorophyll a (Chl a) concentration and heterotrophic bacteria, Synechococcus and mesozooplankton abundance were studied. The results suggested that the auto-and hetero-trophic components of the food web (at least the prokaryotes) were enhanced by the dust addition (and by the nitrogen and phosphorus added through dust). Furthermore, a 1-day delay was observed for PP, BP, and Chl a increases when dust was added in three daily doses; however, the maximal values attained were similar in the two treatments. Although, the effect was evident in the first osmotrophic level (phytoplankton and bacteria), it was lost further up the food web, masked under the impact of grazing exerted by predators such as heterotrophic flagellates, ciliates and dinoflagellates. This was partly proved by two dilution experiments. This study demonstrates the important role of atmospheric deposition and protist grazing when evaluating the effect on oligotrophic systems characterized by increased numbers of trophic levels.

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An optimal manual procedure for ammonia analysis in natural waters by the indophenol blue method

Cited by 355 publications

References 12 publications

Bacterial Growth and Mortality after Deposition of Saharan Dust and Mixed Aerosols in the Eastern Mediterranean Sea: A Mesocosm Experiment

Bacterial Growth and Mortality after Deposition of Saharan Dust and Mixed Aerosols in the Eastern Mediterranean Sea: A Mesocosm Experiment

The metabolic response of pteropods to acidification reflects natural CO<sub>2</sub>-exposure in oxygen minimum zones

Saharan Dust Deposition Effects on the Microbial Food Web in the Eastern Mediterranean: A Study Based on a Mesocosm Experiment

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An optimal manual procedure for ammonia analysis in natural waters by the indophenol blue method

Cited by 355 publications

References 12 publications

Bacterial Growth and Mortality after Deposition of Saharan Dust and Mixed Aerosols in the Eastern Mediterranean Sea: A Mesocosm Experiment

Bacterial Growth and Mortality after Deposition of Saharan Dust and Mixed Aerosols in the Eastern Mediterranean Sea: A Mesocosm Experiment

The metabolic response of pteropods to acidification reflects natural CO&lt;sub&gt;2&lt;/sub&gt;-exposure in oxygen minimum zones

Saharan Dust Deposition Effects on the Microbial Food Web in the Eastern Mediterranean: A Study Based on a Mesocosm Experiment

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The metabolic response of pteropods to acidification reflects natural CO<sub>2</sub>-exposure in oxygen minimum zones