Transmission lines are special types of cables that are used to conduct alternating current (AC) voltages with frequencies in the radio wave spectrum. One such wire is the parallel-plate transmission line that is generally utilized for short and long-distance signal transmissions as it exhibits minimum power loss over the channel. Its behavior, as it transfers AC voltages, is described by its frequency response that is dependent on many parameters, relevantly its conductivity and dielectric constant. The paper aims to identify and analyze the frequency response of a parallel-plate transmission line using different kinds of conductor and insulating materials. Through the use of the RF Toolbox, specifically, in the Matlab software, the researchers were able to analyze the parallel-transmission line in the frequency domain with respect to the time domain. They were able to generate plots of its frequency response with respect to an input signal's amplitude and phase shift. The data resulting will give insight into how different materials can interact with the frequency response of the signal passing through in the transmission line.