An optimised electrochemical biosensor for the label-free detection of C-reactive protein in blood

Bryan, Thomas; Liu, Xiang; Bueno, Paulo Roberto; Davis, Jason J.

doi:10.1016/j.bios.2012.06.051

Cited by 198 publications

(118 citation statements)

References 37 publications

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“…12, clearly demonstrate that this module can be used as a label-free biosensor. While NeutrAvidin itself is not a particularly useful biomarker, due to the mechanism of the biotin-avidin bonding, the results of this model assay demonstrate that this device can be generalized and used in most label-free affinity assays already developed [45]- [47].…”

Section: Label-free Assaymentioning

confidence: 96%

A Multi-Technique Reconfigurable Electrochemical Biosensor: Enabling Personal Health Monitoring in Mobile Devices

Sun

Venkatesh

Hall

2016

IEEE Trans. Biomed. Circuits Syst.

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Abstract-This paper describes the design, performance, and characterization of a reconfigurable, multi-technique electrochemical biosensor designed for direct integration into smartphone and wearable technologies to enable remote and accurate personal health monitoring. By repurposing components from one mode to the next, the biosensor's potentiostat is able reconfigure itself into three different measurements modes to perform amperometric, potentiometric, and impedance spectroscopic tests all with minimal redundant devices. A 3.9×1.65 cm 2 PCB prototype of the module was developed with discrete components and tested using Google's Project Ara modular smartphone. The amperometric mode has a ±1 nA to ±200 μA measurement range. When used to detect pH, the potentiometric mode can achieve a resolution of <0.08 pH units. In impedance measurement mode, the device can measure 50 Ω -10 MΩ and has been shown to have <6° of phase error. This prototype was used to perform several point-of-care health tracking assays suitable for use with mobile devices: 1) Blood glucose tests were conducted and shown to cover the diagnostic range for Diabetes patients (~200 mg/dL). 2) Lactoferrin, a biomarker for urinary tract infections, was detected with a limit of detection of approximately 1 ng/mL. 3) pH tests of sweat were conducted to track dehydration during exercise. 4) EIS was used to determine the concentration of NeutrAvidin via a label-free assay.

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Section: Label-free Assaymentioning

confidence: 96%

A Multi-Technique Reconfigurable Electrochemical Biosensor: Enabling Personal Health Monitoring in Mobile Devices

Sun

Venkatesh

Hall

2016

IEEE Trans. Biomed. Circuits Syst.

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“…9,11 In more recent work we have demonstrated that these capabilities can be incorporated into both the analysis of real patient samples and the simultaneous detection of multiple markers. 11,[17][18][19][20][21] Faradaic EIS assays are almost exclusively based on analysing the parameter of charge transfer resistance (R ct ) obtained by fitting the acquired current signal to a Randles equivalent circuit (see SI Figure 1 for details) , 22,23 while non-faradaic analyses most typically use modulus of the impedance (|Z|), double layer capacitance (C dl ) or phase (ϕ) as sampling functions. 9,[24][25][26][27] In both approaches a phenomenological model based either on an equivalent circuit 28 or continuum scheme derived from microscopic Poisson-Nernst-Plank continuum equations (using conventional or alternative approaches for diffusional activity) [29][30][31] can be applied from which physical parameters can be extracted.…”

Section: Introdutionmentioning

confidence: 97%

“…a generic input/output signal) beyond traditional EIS approaches 11,19,[32][33][34][35] and specifically none which do not require a premodelling of any given interface of interest. We show herein, that, in applying the transfer function concept to EIS datasets it is not only possible to extract analytically potent information but to also automate this without being limited to the confines of a specific physical model.…”

Section: Introdutionmentioning

confidence: 99%

Immittance Electroanalysis in Diagnostics

et al. 2014

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Impedance derived electroanalytical assays are inherently spectroscopic (frequency resolved) and potentially exceedingly sensitive indicators of interfacial change (such as target binding at an appropriate receptor). We introduce here the use of a portfolio of mathematically derived immittance functions and related components, capable, from the same raw data sets, of enabling increased assay sensitivity and markedly shorter assay times in comparison to traditional impedance analyses. The methodology, applied herein to faradaic (redox probe amplified) and non-faradaic assays, requires no equivalent circuit analysis or prior assumption of response. Its focus is to optimize analytical potency and to enable the user to select and apply the most frequency-optimized reporter of interfacial change and to, thereafter, run rapid (optimized) analyses at single frequencies.

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“…It is defined as a group of metabolic diseases where ultimately the body's pancreas does not produce enough insulin or does not properly respond to insulin produced, resulting in high blood sugar levels over a prolonged period. Glucose meters and other POC devices utilize an assortment of methods for detecting and monitoring biomarkers including electrochemical [16][17][18][19][20], magnetic [21][22][23][24][25][26][27][28][29][30], optical [31][32][33][34], label-free spectroscopic analysis [35][36][37][38][39][40][41][42][43], colorimetric [44][45][46][47][48][49], and plasmonic nanoparticle based sensors [50][51][52]. Generally, electrochemical detection uses potentiometric, amperometric, and impedimetric measurements in conjunction with electroactive tags or free flowing electroactive analytes [17][18][19][20] [15,53,54] are examples of electrochemical and colorimet...…”

Section: Current Commercial Poc Technologiesmentioning

confidence: 99%

Surface enhanced Raman spectroscopy (SERS) for in vitro diagnostic testing at the point of care

et al. 2017

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Point-of-care (POC) device development is a growing field that aims to develop low-cost, rapid, sensitive in-vitro diagnostic testing platforms that are portable, selfcontained, and can be used anywhere -from modern clinics to remote and low resource areas. In this review, surface enhanced Raman spectroscopy (SERS) is discussed as a solution to facilitating the translation of bioanalytical sensing to the POC. The potential for SERS to meet the widely accepted "ASSURED" (Affordable, Sensitive, Specific, Userfriendly, Rapid, Equipment-free, and Deliverable) criterion provided by the World Health Organization is discussed based on recent advances in SERS in vitro assay development. As SERS provides attractive characteristics for multiplexed sensing at low concentration limits with a high degree of specificity, it holds great promise for enhancing current efforts in rapid diagnostic testing. In outlining the progression of SERS techniques over the past years combined with recent developments in smart nanomaterials, high-throughput microfluidics, and low-cost paper diagnostics, an extensive number of new possibilities show potential for translating SERS biosensors to the POC.

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An optimised electrochemical biosensor for the label-free detection of C-reactive protein in blood

Cited by 198 publications

References 37 publications

A Multi-Technique Reconfigurable Electrochemical Biosensor: Enabling Personal Health Monitoring in Mobile Devices

A Multi-Technique Reconfigurable Electrochemical Biosensor: Enabling Personal Health Monitoring in Mobile Devices

Immittance Electroanalysis in Diagnostics

Surface enhanced Raman spectroscopy (SERS) for in vitro diagnostic testing at the point of care

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