An optimised method for the rapid analysis of condensed phosphates in fishery and processed marine food products using ion chromatography and microwave sample processing

Detection of polyphosphates in seafood and its relevance toward food safety

“…A typical HPIEC-SCD chromatogram that followed by HRMS profile of 15 main peaks found in SHMP solution (100µg/g) is showed in Figure 2A. Chromatogram obtain by SCD is in the line of recently published finding of Kim et al (2019) where low-chained polyphosphates emerged as the main products. The peaks revealed by the means of HRMS corresponds to the polyphosphates listed in Table 1 and are comparable with only one literature datum available regarding the electrospray mass spectrometry characterisation of polyphosphates (Choi, Hercules & Houalla 2000).…”

“…The presence of SHMP in real food samples was confirmed recently by Kim et al (2019), by means of IC-SCD, but to the best of our knowledge, HRMS has not yet been applied for the SHMP characterisation. IC-SCD-HRMS methodology has been confirmed as a useful tool in the analysis of various inorganic substances in a vast variety of matrices (Chiesa, Panseri, Pasquale, Malandra, Pavlovic & Arioli, 2018;Nobile et al, 2019) with the final objective of utilizing it in food safety and food control purposes, but has not yet been exploited in polyphosphate fingerprinting.…”

“…"Fingerprinting" of complex polymeric linear and cyclic phosphates mixtures such SHMP can be accomplished by IC using 2-mm AS11 column with a KOH gradient (Dionex 2010, Kim et al 2019.…”

“…Generally, it is more likely that orthophosphate derives from the degradation of adenosine triphosphate (ATP). Indeed, many marine edible products contain naturally occurring levels of orthophosphate (Kim et al, 2019). Literature do not give any information on the presence, composition, and way in which any of eventually added polyphosphates could interact with cuttlefish tissues, nor whether they breakdown over time results in producing PO 4 -3 .…”

“…The most important literature data where IEC has been used for determination of polyphosphates in seafood are listed in the Table 1S. IC remains a priority choice for the analysis of (poly)phosphates because it was proven to be highly sensitive that is able to separate the main (poly)phosphates species (Cui, Cai, & Xu, 2000;Sekiguchi et al, 2000;Kafumann, Maden, Leisser, Matera, & Gude, 2005;Iammarino & Di Taranto, 2012;Kim et al 2019).…”