2023
DOI: 10.1101/2023.12.03.569756
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An optimised protocol for quality control of gene therapy vectors using Nanopore direct RNA sequencing

Kathleen Zeglinski,
Christian Montellese,
Matthew E. Ritchie
et al.

Abstract: Despite recent advances made towards improving the efficacy of lentiviral gene therapies, a sizeable proportion of produced vector contains an incomplete and thus potentially non-functional RNA genome. This can undermine gene delivery by the lentivirus as well as increase manufacturing costs and must be improved to facilitate the widespread clinical implementation of lentiviral gene therapies. Here, we compare three long-read sequencing technologies for their ability to detect issues in vector design and deter… Show more

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