An optimized five-color/seven-parameter flow cytometry panel for immunophenotyping guinea pig peripheral blood lymphocytes

Crawford, Anna E.; Cross, Claire E.; Ross, Anne-Marie L.; Walker, Jamie D.; Willeford, Bridget V.; Varela‐Stokes, Andrea S.

doi:10.1016/j.jim.2019.112682

Cited by 6 publications

(4 citation statements)

References 14 publications

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“…The sensitivity of guinea pigs to infections and the similarities of their immune system to that of humans have made this animal model important in the study of human infectious diseases ( 21 – 23 ). Between the 1950s and 1990s, the guinea pig was used as a model for MuV research.…”

Section: Discussionmentioning

confidence: 99%

“…However, in all of those studies the only immunological parameter that was investigated was the humoral immune response. Development of new tools for detection of humoral and cellular immune responses such as guinea pig-specific IFN-γ ELISpot assay ( 34 ) or improving antibody selection for flow cytometry analysis ( 23 ) provided a better insight into the development of cellular immunity in guinea pigs during experimental MuV infection, as well as long-term immunity. For all the above reasons, in this study we decided to examine the guinea pig and its interaction with MuV in more detail.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of the Interactions between Mumps Virus and Guinea Pig

Balija

Štimac

Gulija

et al. 2023

J Virol

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Evaluation of the Interactions between Mumps Virus and Guinea Pig

Balija

Štimac

Gulija

et al. 2023

J Virol

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“…Interestingly, the allergen-induced airway obstruction in guinea pigs involves both early and late allergic response, that make it similar to humans and dissimilar from mice [ 110 ]. Although guinea pig models of asthma and COPD are clinically superior to rodents [ 111 ], their use is still limited due to the paucity of available reagents and lack of genetically manipulated strains [ 112 ].…”

Section: Discussionmentioning

confidence: 99%

Muscarinic receptor antagonists and airway inflammation: A systematic review on pharmacological models

Calzetta

Pistocchini

Ritondo

et al. 2022

Heliyon

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“…We included a viability dye to exclude dead cells from the analysis. Guinea pig serum was previously determined to effectively block Fc receptors at a final concentration of 5% in the staining buffer [26], and monocyte blocker was used to prevent non-specific binding of PE-Cy7 to monocytes. We assessed positivity using Fluorescent Minus One (FMO) controls, as seen in Supplementary Figure S3.…”

Section: Panel Designmentioning

confidence: 99%

Skin in the Game: An Assay to Monitor Leukocyte Infiltration in Dermal Lesions of a Guinea Pig Model for Tick-Borne Rickettsiosis

et al. 2022

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Intact, the skin typically serves as an effective barrier to the external world; however, once pathogens have breached this barrier via a wound, such as a tick bite, the surrounding tissues must recruit immune cells from the blood to neutralize the pathogen. With innate and adaptive immune systems being similar between the guinea pig and human systems, the ability of guinea pigs to show clinical signs of many infectious diseases, and the large size of guinea pigs relative to a murine model, the guinea pig is a valuable model for studying tick-borne and other pathogens that invade the skin. Here, we report a novel assay for assessing guinea pig leukocyte infiltration in the skin. Briefly, we developed an optimized six-color/eight-parameter polychromatic flow cytometric panel that combines enzymatic and mechanical dissociation of skin tissue with fluorescent antibody staining to allow for the immunophenotyping of guinea pig leukocytes that have migrated into the skin, resulting in inflammation. We designed this assay using a guinea pig model for tick-borne rickettsiosis to further investigate host–pathogen interactions in the skin, with preliminary data demonstrating immunophenotyping at skin lesions from infected ticks. We anticipate that future applications will include hypothesis testing to define the primary immune cell infiltrates responding to exposure to virulent, avirulent tick-borne rickettsiae, and tick-borne rickettsiae of unknown virulence. Other relevant applications include skin lesions resulting from other vector-borne pathogens, Staphylococcus aureus infection, and Buruli ulcer caused by Mycobacterium ulcerans.

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An optimized five-color/seven-parameter flow cytometry panel for immunophenotyping guinea pig peripheral blood lymphocytes

Cited by 6 publications

References 14 publications

Evaluation of the Interactions between Mumps Virus and Guinea Pig

Evaluation of the Interactions between Mumps Virus and Guinea Pig

Muscarinic receptor antagonists and airway inflammation: A systematic review on pharmacological models

Skin in the Game: An Assay to Monitor Leukocyte Infiltration in Dermal Lesions of a Guinea Pig Model for Tick-Borne Rickettsiosis

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