An optimized method to assess in vivo efficacy of antithrombotic drugs using optical coherence tomography and a modified Doppler flow system

Couture, Lucie; Richer, Louis-Philippe; Cadieux, C. Linn; Thomson, C.M.; Hossain, S.M.

doi:10.1016/j.vascn.2011.09.001

Cited by 10 publications

(9 citation statements)

References 21 publications

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“…Comparatively, the other methods are only effective at and above 100 to 300 μm level, which means small vessels are hardly visible not to mention imaging and quantifying thrombus within these vessels. OCT has been employed to examine thrombus morphology ex vivo . Intracoronary OCT has been developed to both identify coronary atherosclerosis, thrombus formation and plaque rupture due to its distinct resolution advantage over other imaging modalities .…”

Section: Resultsmentioning

confidence: 99%

“…Our system allows us to determine thrombus size and cross section area (represented by percentage of occlusion), time to occlusion as well as time to thrombus size, all parameters are highly valuable to examine thrombus morphology from different aspects. Whereas previous studies usually utilize analytic scale, intravital microscopy and Doppler flow meter to obtain individual parameter such as thrombus weight, thrombus length or time to occlusion, respectively . Furthermore, by using OCT technique, all these measurements can be followed over time in the same mouse, which allows us to appreciate the kinetics of clot formation without sacrificing animals and in the meanwhile limit the number of mice to the minimum.…”

Section: Resultsmentioning

confidence: 99%

“…Postmortem histopathological analysis of thrombi weight and length relies on examination after dissecting thrombi , because thrombosis progression was not monitored in the same mouse over time under this circumstance, the drawbacks are obvious: time‐consuming and large number of mice are sacrificed. Time to occlusion reflects the amount of time it takes for the thrombus to completely block vessel blood flow but it also does not give thrombodynamics information .…”

Section: Introductionmentioning

confidence: 99%

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In vivo monitoring of thrombosis in mice by optical coherence tomography

Liu

et al. 2019

Journal of Biophotonics

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The objective of this study is to establish a novel method for continuously monitoring thrombus progression with various outcome measures and to assess the efficacy of antithrombotic drugs in murine thrombosis model in mice. In the study, thrombus was induced in the femoral vein of mice by FeCl 3 and monitored over time by spectraldomain optical coherence tomography (OCT). Three-dimensional images of thrombi with or without heparin as an antithrombotic agent were obtained from OCT angiography. In addition, several parameters of thrombi were analyzed and compared between control and anticoagulant groups. By using OCT, we were able to trace thrombus generation in the same mouse in real time. We found that in our model heparin reduced thrombus size by~60% and thrombus cross-sectional area by 50%. OCT results also show that both time to thrombus size (>0.02mm 3 ) and time to occlusion (>30%) were significantly reduced after heparin addition. This study demonstrates that OCT reliably monitors thrombus generation and progression from various aspects including thrombus size. This enables us to measure the kinetic of thrombosis more accurately, and effectively evaluate the efficacy and activities of antithrombotic drugs.This model may represent a useful tool in antithrombotic drug discoveries in preclinical studies. K E Y W O R D Santithrombotic drugs, mice, optical coherence tomography, thrombosis

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In vivo monitoring of thrombosis in mice by optical coherence tomography

Liu

et al. 2019

Journal of Biophotonics

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“…Currently, no precise profile of real-time RAT during TRI has been reported because of observational limitations. Optical coherence tomography (OCT) has proven to be a reliable method to assess intravascular device-related thrombi in coronary intervention in the last decades [ 2 ]. A few OCT studies have revealed a remarkably high incidence of RAT from 20.5 to 23.5% during TRI procedures [ 3 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

Bivalirudin vs. Heparin on Radial Artery Thrombosis during Transradial Coronary Intervention: An Optical Coherence Tomography Study

Liu

Wang

Niu

et al. 2020

Journal of Interventional Cardiology

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Objectives. This study aimed to evaluate the antithrombotic efficacy between bivalirudin and unfractionated heparin (UFH) on radial artery thrombosis (RAT) during transradial coronary intervention (TRI) by optical coherence tomography (OCT). Methods and Results. We consecutively reviewed a total of 307 patients who underwent radial artery OCT inspection after TRI in our centre from October 2017 to January 2019; afterwards, 211 screened patients were divided into the UFH group (n = 144) and the bivalirudin group (n = 67) according to their anticoagulation strategy during TRI. The thrombosis in the radial artery was observed in 51 cases (24.17%) with a median thrombus volume of 0.054 mm3 (0.024, 0.334) and median thrombus score of 7 (4, 15). Thrombus occurred in 28 cases in the bivalirudin group with an incidence of 41.8%, which was significantly higher than that in the UFH group (n = 23, 16.0%, P<0.001). This difference was even more remarkable after propensity score matching (bivalirudin group n = 22, 42.3% vs. UHF group n = 11, 13.9%, P<0.001). Multivariate logistic analysis revealed that bivalirudin increased the RAT risk by 3.872 times (95% CI 2.006–8.354, P<0.001) after adjustment for the other predictors. Conclusion. In this present study, the use of bivalirudin was associated with a higher risk of RAT than UFH. It highlighted UFH should be a more considerable choice to prevent radial artery access thrombosis in TRI.

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“…9 The technique has the major advantage of providing a description of whole blood properties with high resolution, high sensitivity, and potential application in vivo, such as characterizing blood optical clearing, hematocrit (HCT), red blood cells (RBCs) aggregation, blood photocoagulation, blood oxygen saturation, thrombus volume, and blood fibrinogen level. [10][11][12][13][14][15][16][17][18] Recently, we reported that OCT technique was able to characterize the whole blood coagulation process and the effects of anticoagulants on blood coagulation in the static state. 19,20 The 1∕e light penetration depth (d 1∕e ) [the point where the signal attenuated to 37% (1∕e) of the point with the greatest reflectance] versus time of blood coagulation process was able to differentiate various stages of blood properties during coagulation in the static state.…”

Section: Introductionmentioning

confidence: 99%

Monitoring the blood coagulation process under various flow conditions with optical coherence tomography

Geng

Teng

2014

J. Biomed. Opt

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Our previous work demonstrated that an optical coherence tomography (OCT) technique was able to characterize the whole blood coagulation process. The 1/e light penetration depth (d(1/e)) derived from the profiles of reflectance versus depth was developed for detecting the whole blood coagulation process in static state. To consider the effect of blood flow, in the present study, d(1/e) versus time from the coagulating porcine blood circulated in a mock flow loop with various steady laminar flows at mean flow speed in the range from 5 to 25 mm/s. The variation of d(1/e) was used to represent the change of blood properties during coagulation in different hematocrits (HCT) ranging from 25% to 55%, velocities from 5 to 25 mm/s, and tubing sizes from 0.9 to 2 mm. The results showed that there were positive correlations between coagulation time (t(c)) and HCT, velocity, and tubing size, respectively. In addition, the coagulation rate (S(r)) was decreased with the increase of HCT, velocity, and tubing size. This study testified that HCT, flow velocity, and tubing size were substantial factors affecting the backscattering properties during flowing blood coagulation. Furthermore, OCT has the potential to represent the process of flowing blood coagulation with proper parameters.

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An optimized method to assess in vivo efficacy of antithrombotic drugs using optical coherence tomography and a modified Doppler flow system

Cited by 10 publications

References 21 publications

In vivo monitoring of thrombosis in mice by optical coherence tomography

In vivo monitoring of thrombosis in mice by optical coherence tomography

Bivalirudin vs. Heparin on Radial Artery Thrombosis during Transradial Coronary Intervention: An Optical Coherence Tomography Study

Monitoring the blood coagulation process under various flow conditions with optical coherence tomography

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