An optimized protocol for estimating cellulase activity in biological samples

Talebi, Zainab Abbas Al; Al-Kawaz, Hawraa Saad; Mahdi, Rasha Kadhim; Al-Hassnawi, Alaa Tariq; Alta'ee, Abdulsamie H.; Hadwan, Asad M.; khudhair, Dunia Abbas; Hadwan, Mahmoud Hussein

doi:10.1016/j.ab.2022.114860

Cited by 4 publications

(2 citation statements)

References 54 publications

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“…Cellulases produced by certain microorganisms can play a key role in the degradation of cellulose present in stone cells (Ajeje et al, 2021; Ren et al, 2019). These cellulases include exoglucanase, endoglucanase, and β‐glucosidase (Talebi et al, 2022). Studies have shown that clear zone test on CMC medium with iodine and the filter paper degradation test are the most common methods to test the production of cellulase by microbial strains (Kurnia Ila & Astuti Feb, 2021).…”

Section: Discussionmentioning

confidence: 99%

A study revealing mechanisms behind the stone cell of Yali pear degradation by mixed‐culture fermentation of lactic acid bacteria and yeast

Gao,

Xu,

Tian

et al. 2024

eFood

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The presence of stone cells in pears is recognized as a problem for the pear processing industry. Bacillus and mold can degrade stone cells because of their potential to digest cellulose and lignin, but they cannot be used for the degradation test of pear stone cells. In this study Lactoplantibacillus plantarum JYLP‐326 was used in single culture or in the mixed culture with Saccharomyces cerevisiae Y2 to study their potential to degrade stone cells during fermentation and the mechanism of degradation was further explored. Synergy in cellulase activity was observed in the mixed‐culture where the maximum activity was observed at 96th hour of fermentation. Activities of endoglucanase, exoglucanase and β‐glucosidase were 1.75, 4.58, and 2.31. The degradation rate of stone cells in the mixed‐culture was 37.67%, which was significantly higher than that the results obtained for single cultures. The results of scanning electron microscopy (SEM) showed that the surface of the cultured stone cells became rough. Metabolomics studies confirmed the presence of specific metabolites related to the degradation of stone cells after the fermentation. It was concluded that the mixed‐culture fermentation using the above‐mentioned strains could be exploited by the pear processing industry to degrade stone cells.

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Section: Discussionmentioning

confidence: 99%

A study revealing mechanisms behind the stone cell of Yali pear degradation by mixed‐culture fermentation of lactic acid bacteria and yeast

Gao,

Xu,

Tian

et al. 2024

eFood

View full text Add to dashboard Cite

show abstract

“…A 2.0 mL sample of the 1-month-subcultured microflora was centrifuged at 14,000× g and 4 • C for 10 min. The resultant supernatant, termed enzyme solution, was analyzed for the presence of cellulase, chitinase, and pectinase using the modified dinitro salicylic acid (DNS) method [21]. In brief, 200 µL of the enzyme solution and 800 µL of 50 mM citrate buffer (pH 4.8) containing 12.5 mg/mL of carboxymethyl cellulose, chitin from shrimp shells, or citrus pectin, as a substrate for cellulase, chitinase, or pectinase, respectively, were mixed in a microtube and incubated for 1 h at 50 • C. The tubes were then centrifuged at 14,000× g and 4 • C for 10 min.…”

Section: Enzymatic Assaymentioning

confidence: 99%

The Potential of Digested Sludge-Assimilating Microflora for Biogas Production from Food Processing Wastes

Hasaka,

Sakamoto,

Fujii

2023

Microorganisms

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Food processing wastes (FPWs) are residues generated in food manufacturing, and their composition varies depending on the type of food product being manufactured. Therefore, selecting and acclimatizing seed microflora during the initiation of biogas production is crucial for optimal outcomes. The present study examined the biogas production capabilities of digested sludge-assimilating and biogas-yielding soil (DABYS) and enteric (DABYE) microflorae when used as seed cultures for biogas production from FPWs. After subculturing and feeding these microbial seeds with various FPWs, we assessed their biogas-producing abilities. The subcultures produced biogas from many FPWs, except orange peel, suggesting that the heterogeneity of the bacterial members in the seed microflora facilitates quick adaptation to FPWs. Microflorae fed with animal-derived FPWs contained several methanogenic archaeal families and produced methane. In contrast, microflorae fed with vegetable-, fruit-, and crop-derived FPWs generated hydrogen, and methanogenic archaeal populations were diminished by repeated subculturing. The subcultured microflorae appear to hydrolyze carbohydrates and protein in FPWs using cellulase, pectinase, or protease. Despite needing enhancements in biogas yield for future industrial scale-up, the DABYS and DABYE microflorae demonstrate robust adaptability to various FPWs.

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Cellulolytic potential of mangrove bacteria Bacillus haynesii DS7010 and the effect of anthropogenic and environmental stressors on bacterial survivability and cellulose metabolism

Palit,

Das

2024

Environmental Research

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An optimized protocol for estimating cellulase activity in biological samples

Cited by 4 publications

References 54 publications

A study revealing mechanisms behind the stone cell of Yali pear degradation by mixed‐culture fermentation of lactic acid bacteria and yeast

A study revealing mechanisms behind the stone cell of Yali pear degradation by mixed‐culture fermentation of lactic acid bacteria and yeast

The Potential of Digested Sludge-Assimilating Microflora for Biogas Production from Food Processing Wastes

Cellulolytic potential of mangrove bacteria Bacillus haynesii DS7010 and the effect of anthropogenic and environmental stressors on bacterial survivability and cellulose metabolism

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