An Optimized Terpene Profile for a New Medical Cannabis Oil

Abstract

The purpose of this analytical study was to develop an advanced formulation of medical Cannabis oil (MCO) comparing the chemical profile of different extracts obtained with two existing methods (SIFAP and CALVI) and one original upgraded (CERFIT) method. Preparation methods were applied with varying solvent, temperature, and duration of the decarboxylation and extraction steps. HPLC-MS/MS TSQ and GC/FID-HS analyses were performed to investigate cannabinoid and terpene contents in the three oil extracts. Cannabinoids profile remained comparable between the formulations. CERFIT extracts exhibited a superior quantity of total terpene hydrocarbon forms (e.g., limonene and α-pinene) with no degradation occurrence (i.e., oxidized terpenes not quantifiable). Thus, this new method optimized the phytochemical profile of the MCO presenting a value opportunity to obtain a standardized high-level therapeutic product.

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“…During the dissolution process, the finely pulverized edible sample is combined with the chosen solvent to commence the extraction. Several factors can impact this process, including the solvent temperature, the contact duration between the sample and the solvent, and the level of agitation applied to the mixture. , After the dissolution process, the mixture is subjected to dispersion to evenly distribute the cannabinoids in the solvent. The dispersion process is effectively accomplished by thorough mixing aided by techniques such as stirring, shaking, and ultrasonication.…”

Are cannabidiol (CBD) levels in consumer food products well tested?—A review

“…They are also known to exist in plants of the species Radula and Helichrysum . In addition to cannabinoids, non‐cannabinoid phytochemicals such as flavonoids and terpenes are also found (Maggini et al., 2022). In the group of secondary metabolites, there are cannabin alkaloids (cannabisavin and anhydrocannabizavin), which, in addition to analgesic properties, have a pronounced antioxidant potential (Atalay et al., 2019; Huestis et al., 2019; Jin et al., 2020).…”

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