An oral double-targeted DNA vaccine induces systemic and intestinal mucosal immune responses and confers high protection against Vibrio mimicus in grass carps

Cao, Ji; Zhu, Xinchi; Liu, Xinyuan; Yuan, Kun; Zhang, Jiajun; Gao, Huile; Li, Jinnian

doi:10.1016/j.aquaculture.2019.02.006

Cited by 23 publications

(5 citation statements)

References 37 publications

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“…LZM is an important defense component of the innate immune system, which plays a crucial role in mediating protection against bacterial and viral invasion (41,42). A previous study reported that LZM activity in the serum and intestinal mucus of grass carp increased significantly after immunization with an oral DNA vaccine compared to control fish (43). In our study, the LZM activity of the immunized fish was significantly higher in the serum, foregut, and hindgut mucus.…”

Section: Discussionsupporting

confidence: 57%

Gut mucosal immune responses and protective efficacy of oral yeast Cyprinid herpesvirus 2 (CyHV-2) vaccine in Carassius auratus gibelio

Dong

Kong

et al. 2022

Front. Immunol.

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Cyprinid herpesvirus 2 (CyHV-2) causes herpesviral hematopoietic necrosis (HVHN) disease outbreaks in farmed Cyprinid fish, which leads to serious economic losses worldwide. Although oral vaccination is considered the most suitable strategy for preventing infectious diseases in farmed fish, so far there is no commercial oral vaccine available for controlling HVNN in gibel carp (C. auratus gibelio). In the present study, we developed for the first time an oral vaccine against CyHV-2 by using yeast cell surface display technology and then investigated the effect of this vaccine in gibel carp. Furthermore, the protective efficacy was evaluated by comparing the immune response of a single vaccination with that of a booster vaccination (booster-vaccinated once 2 weeks after the initial vaccination). Critically, the activities of immune-related enzymes and genes expression in vaccine group, especially in the booster vaccine group, were higher than those in the control group. Moreover, strong innate and adaptive immune responses could be elicited in both mucosal and systemic tissues after receipt of the oral yeast vaccine. To further understand the protective efficacy of this vaccine in gibel carp, we successfully developed the challenge model with CyHV-2. Our results showed the relative percent survival was 66.7% in the booster vaccine group, indicating this oral yeast vaccine is a promising vaccine for controlling CyHV-2 disease in gibel carp aquaculture.

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Section: Discussionsupporting

confidence: 57%

Gut mucosal immune responses and protective efficacy of oral yeast Cyprinid herpesvirus 2 (CyHV-2) vaccine in Carassius auratus gibelio

Dong

Kong

et al. 2022

Front. Immunol.

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“…These results show that DNA vaccines prepared with BG-loaded plasmids have a better stimulatory effect on both humoral and cellular immunities, relative to naked DNA vaccines. Cao et al used DH5α ghosts loaded with plasmids containing five exogenous fragments (including invariant chain-like protein [Iclp] gene) to prepare double-targeted DNA vaccines for oral immunization of grass carp [81]. Plasmids with endogenous Iclp easily enter the MHC-II antigen presentation pathway.…”

Section: Bgs-based Vaccine 41 Dna Vaccinesmentioning

confidence: 99%

Bacterial Ghosts-Based Vaccine and Drug Delivery Systems

Chen

Kong

et al. 2021

Pharmaceutics

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Bacterial ghosts (BGs) are empty bacterial envelopes of Gram-negative bacteria produced by controlled expressions of cloned gene E, forming a lysis tunnel structure within the envelope of the living bacteria. Globally, BGs have been used as vaccine delivery systems and vaccine adjuvants. There is an increasing interest in the development of novel delivery systems that are based on BGs for biomedical applications. Due to intact reservation of bacterial cell membranes, BGs have an inherent immunogenicity, which enables targeted drug delivery and controlled release. As carrier vehicles, BGs protect drugs from interference by external factors. In recent years, there has been an increasing interest in BG-based delivery systems against tumors, inflammation, and infection, among others. Herein, we reviewed the preparation methods for BGs, interactions between BGs and the host, and further highlighted research progress in BG development.

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“…T-SOD, AKP, ACP, and LZM are generally selected as indicators to evaluate the immune status and disease resistance of fish. Previously, it was reported that the oral double-targeted DNA vaccine of Vibrio mimicus could significantly increase the activity of SOD and LZM in the serum and intestinal mucus of grass carp ( Ctenopharyngodon idella ) [ 50 ]. The oral Bacillus subtilis vaccine expressing VP56 of grass carp reovirus II (GCRVII) markedly increased the activity of immune-related enzymes in the serum and intestine of immunized grass carp [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Oral Vaccination of Largemouth Bass (Micropterus salmoides) against Largemouth Bass Ranavirus (LMBV) Using Yeast Surface Display Technology

Zhang

Chen

Xue

et al. 2023

Animals

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Largemouth bass ranavirus (LMBV) infects largemouth bass, leading to significant mortality and economic losses. There are no safe and effective drugs against this disease. Oral vaccines that directly target the intestinal mucosal immune system play an important role in resisting pathogens. Herein, the B subunit of Escherichia coli heat-labile enterotoxin (LTB, a mucosal immune adjuvant) and the LMBV main capsid protein (MCP) were expressed using Saccharomyces cerevisiae surface display technology. The yeast-prepared oral vaccines were named EBY100-OMCP and EBY100-LTB-OMCP. The candidate vaccines could resist the acidic intestinal environment. After 7 days of continuous oral immunization, indicators of innate and adaptive immunity were measured on days 1, 7, 14, 21, 28, 35, and 42. High activities of immune enzymes (T-SOD, AKP, ACP, and LZM) in serum and intestinal mucus were detected. IgM in the head kidney was significantly upregulated (EBY100-OMCP group: 3.8-fold; BY100-LTB-OMCP group: 4.3-fold). IgT was upregulated in the intestines (EBY100-OMCP group: 5.6-fold; EBY100-LTB-OMCP group: 6.7-fold). Serum neutralizing antibody titers of the two groups reached 1:85. Oral vaccination protected against LMBV infection. The relative percent survival was 52.1% (EBY100-OMCP) and 66.7% (EBY100-LTB-OMCP). Thus, EBY100-OMCP and EBY100-LTB-OMCP are promising and effective candidate vaccines against LMBV infection.

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An oral double-targeted DNA vaccine induces systemic and intestinal mucosal immune responses and confers high protection against Vibrio mimicus in grass carps

Cited by 23 publications

References 37 publications

Gut mucosal immune responses and protective efficacy of oral yeast Cyprinid herpesvirus 2 (CyHV-2) vaccine in Carassius auratus gibelio

Gut mucosal immune responses and protective efficacy of oral yeast Cyprinid herpesvirus 2 (CyHV-2) vaccine in Carassius auratus gibelio

Bacterial Ghosts-Based Vaccine and Drug Delivery Systems

Oral Vaccination of Largemouth Bass (Micropterus salmoides) against Largemouth Bass Ranavirus (LMBV) Using Yeast Surface Display Technology

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