An orally delivered, live‐attenuated <i>Edwardsiella ictaluri</i> vaccine efficiently protects channel catfish fingerlings against multiple <i>Edwardsiella ictaluri</i> field isolates

Aarattuthodiyil, Suja; Griffin, Matt J.; Greenway, Terrence E.; Khoo, Lester H.; Byars, Todd S.; Lewis, Marsha; Steadman, James; Wise, David J.

doi:10.1111/jwas.12693

Cited by 15 publications

(16 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This phenomenon has been described in similar studies investigating cross‐protective immunity in fish vaccines (Poobalane et al., 2010; Wang et al., 2013). In Experiment 1, post‐vaccination, prechallenge losses were inconsistent with previous studies (Aarattuthodiyil et al., 2020; Chatakondi et al., 2018; Greenway et al., 2017; Griffin, Greenway, et al., 2020; Peterson et al., 2016; Wise et al., 2015). This response could be attributed to the extremely high vaccine dose (>1 × 10 7 CFU/ml) and increased exposure time used to ensure an adequate immune response in hybrids, which are typically less susceptible to E .…”

Section: Discussioncontrasting

confidence: 69%

Cross‐protective efficacy of a live‐attenuated Edwardsiella ictaluri vaccine against heterologous Edwardsiella piscicida isolates in channel and channel × blue catfish hybrids

López-Porras

Griffin

Ware

et al. 2022

Journal of Fish Diseases

Self Cite

View full text Add to dashboard Cite

Edwardsiella piscicida is a growing problem for catfish aquaculture in the southeastern United States, particularly in channel (Ictalurus punctatus) x blue (I. furcatus) catfish hybrids. Research has shown E. piscicida isolates recovered from farmed catfish in Mississippi form at least five discrete phyletic groups, with no apparent differences in virulence in channel and hybrid catfish. Laboratory trials have shown a live‐attenuated E. ictaluri vaccine (340X2) cross‐protects against at least one E. piscicida isolate (S11–285) in channel and hybrid catfish, although it is unknown if this protection exists for other E. piscicida variants. To this end, channel and hybrid catfish were immunized by immersion with E. ictaluri 340X2. Thirty days later, fish were challenged by intracoelomic injection with representative E. piscicida variants from each phyletic group. Relative percent survival (RPS) for hybrids ranged from 54.7% to 77.8%, while RPS in channels ranged from 80.5% to 100%. A second study investigated whether channel and hybrid catfish exposed to heterologous E. piscicida isolates were similarly protected against wild‐type E. ictaluri. Fish were exposed by bath immersion to representative E. piscicida isolates from each phyletic group. Thirty days post‐immunization, fish were challenged by immersion with wild‐type E. ictaluri isolate S97–773. Regardless of variant, previous exposure to heterologous E. piscicida isolates significantly improved survival following E. ictaluri challenge. These findings suggest the presence of shared and conserved antigens among E. piscicida and E. ictaluri that could be exploited by application of polyvalent or cross‐protective vaccines.

show abstract

Section: Discussioncontrasting

confidence: 69%

Cross‐protective efficacy of a live‐attenuated Edwardsiella ictaluri vaccine against heterologous Edwardsiella piscicida isolates in channel and channel × blue catfish hybrids

López-Porras

Griffin

Ware

et al. 2022

Journal of Fish Diseases

Self Cite

View full text Add to dashboard Cite

show abstract

“…Contrary to this line of thought, Klesius and Shoemaker () reported that channel catfish immunized by sublethal exposure to a virulent E. ictaluri isolate were not protected against all challenge isolates in a heterologous cross‐protection study. Comparably, the vaccine isolate used here was shown to protect fish against 23 temporally discrete isolates from U.S. catfish operations archived from 1993 to 2016 (Aarattuthodiyil et al, ). Moreover, using similarly produced vaccine serials, genetic variation (family effects) in host fish was not shown to influence vaccine efficacy (Peterson et al, ).…”

Section: Discussionmentioning

confidence: 94%

Validation of Edwardsiella ictaluri oral vaccination platform in experimental pond trials

Wise

Greenway

Byars

et al. 2019

J World Aquaculture Soc

Self Cite

View full text Add to dashboard Cite

Since its recognition in the early 1980s, enteric septicemia of catfish (ESC), caused by the gram-negative enteric Edwardsiella ictaluri, is one of the most significant pathogens affecting catfish aquaculture. In efforts to improve disease management in U.S. catfish aquaculture, a live-attenuated ESC vaccine and method of in-pond oral vaccine delivery was developed. This work evaluated the efficacy of the oral delivery platform using a live-attenuated E. ictaluri vaccine under simulated commercial conditions. Channel catfish fingerlings were orally vaccinated approximately 40-50 days poststocking by mixing the attenuated vaccine with feed using a patent-pending mechanized delivery system. Across three discrete years under varying evaluation methods, vaccination resulted in significant improvements in survival, feed conversion ratio, feed fed, and total yield. An economic analysis of experimental data suggests a significant positive net economic benefit from vaccination.After accounting for a projected cost of vaccination, the realized improvements in survival and yield resulted in an estimated benefit exceeding $3,500/ha ($1,400/acre) on fingerling operations. These results suggest vaccinating channel catfish fingerlings against ESC is an effective strategy to mitigate ESC-related losses and can significantly improve production efficiency and profitability on catfish operations.

show abstract

“…For example, there is evidence that a live-attenuated E. ictaluri vaccine (Wise et al, 2015) can infer protection against E. piscicida in channel and hybrid catfish, although this has only been demonstrated against E. piscicida isolate S11-285, a member of the common MLSA clade 1 (Griffin et al, 2020). Although the E. ictaluri vaccine is effective against multiple E. ictaluri isolates from catfish farms in Mississippi and Alabama, molecular analysis revealed these geographically and temporally discrete isolates collected from 1993 to 2006 were largely clonal (Aarattuthodiyil et al, 2020). Given the genetic plasticity of E. piscicida in catfish aquaculture, it is prudent to evaluate protection against other E. piscicida phylogroups in future studies of E. piscicida variants from channel and hybrid catfish aquaculture.…”

Section: Discussionmentioning

confidence: 99%

Genetic variability of Edwardsiella piscicida isolates from Mississippi catfish aquaculture with an assessment of virulence in channel and channel × blue hybrid catfish

López-Porras

Griffin

Armwood

et al. 2021

Journal of Fish Diseases

Self Cite

View full text Add to dashboard Cite

The bacterium Edwardsiella piscicida causes significant losses in global aquaculture, particularly channel (Ictalurus punctatus) × blue (I. furcatus) hybrid catfish cultured in the south-eastern United States. Emergence of E. piscicida in hybrid catfish is worrisome given current industry trends towards increased hybrid production. The project objectives were to assess intraspecific genetic variability of E. piscicida isolates recovered from diseased channel and hybrid catfish in Mississippi; and determine virulence associations among genetic variants. Repetitive extragenic palindromic sequencebased PCR (rep-PCR) using ERIC I and II primers was used to screen 158 E. piscicida diagnostic case isolates. A subsample of 39 E. piscicida isolates, representing predominant rep-PCR profiles, was further characterized using BOX and (GTG) 5 rep-PCR primers, virulence gene assessment and multilocus sequence analysis (MLSA) targeting housekeeping genes gyrb, pgi and phoU. The MLSA provided greater resolution than rep-PCR, revealing 5 discrete phylogroups that correlated similarly with virulence gene profiles. Virulence assessments using E. piscicida representatives from each MLSA group resulted in 14-day cumulative mortality ranging from 22% to 54% and 63 to 72% in channel and hybrid fingerlings, respectively. Across all phylogroups, mortality was higher in hybrid catfish (p < .05), supporting previous work indicating E. piscicida is an emerging threat to hybrid catfish aquaculture in the south-eastern United States.

show abstract

An orally delivered, live‐attenuated Edwardsiella ictaluri vaccine efficiently protects channel catfish fingerlings against multiple Edwardsiella ictaluri field isolates

Abstract: The commercial channel catfish (Ictalurus punctatus) industry is adversely impacted by enteric septicemia of catfish (ESC), caused by the Gram-negative enteric rod Edwardsiella ictaluri. In efforts to develop more effective management

Cited by 15 publications

References 65 publications

Cross‐protective efficacy of a live‐attenuated Edwardsiella ictaluri vaccine against heterologous Edwardsiella piscicida isolates in channel and channel × blue catfish hybrids

Cross‐protective efficacy of a live‐attenuated Edwardsiella ictaluri vaccine against heterologous Edwardsiella piscicida isolates in channel and channel × blue catfish hybrids

Validation of Edwardsiella ictaluri oral vaccination platform in experimental pond trials

Genetic variability of Edwardsiella piscicida isolates from Mississippi catfish aquaculture with an assessment of virulence in channel and channel × blue hybrid catfish

Contact Info

Product

Resources

About