An Organoselenium Compound Inhibits Staphylococcus aureus Biofilms on Hemodialysis Catheters In Vivo

Abstract

Colonization of central venous catheters (CVCs) by pathogenic bacteria leads to catheter-related bloodstream infections (CRBSIs). These colonizing bacteria form highly antibiotic-resistant biofilms. Staphylococcus aureus is one of the most frequently isolated pathogens in CRBSIs. Impregnating CVC surfaces with antimicrobial agents has various degrees of effectiveness in reducing the incidence of CRBSIs. We recently showed that organoselenium covalently attached to disks as an antibiofilm agent inhibited the development of S. aureus biofilms. In this study, we investigated the ability of an organoselenium coating on hemodialysis catheters (HDCs) to inhibit S. aureus biofilms in vitro and in vivo. S. aureus failed to develop biofilms on HDCs coated with selenocyanatodiacetic acid (SCAA) in either static or flowthrough continuous-culture systems. The SCAA coating also inhibited the development of S. aureus biofilms on HDCs in vivo for 3 days. The SCAA coating was stable and nontoxic to cell culture or animals. This new method for coating the internal and external surfaces of HDCs with SCAA has the potential to prevent catheter-related infections due to S. aureus.

Garlic ointment inhibits biofilm formation by bacterial pathogens from burn wounds

“…2b). Quantitative analysis of the biofilm using the COMSTAT program revealed a biomass (35.35 mm 3 per mm 2 ), mean thickness (120.79 mm) and surface area (2.15610 7 mm 2 ) similar to P. aeruginosa biofilms in the flow-through continuous-culture system (Schaber et al, 2007) and S. aureus AH133 biofilms on haemodialysis catheters in vivo (Tran et al, 2012). Quantification of the bacteria present showed a 4-5 log increase in the number of micro-organisms from 10 3 -10 4 c.f.u.…”

Organo-Selenium-containing Dental Sealant Inhibits Bacterial Biofilm

“…We examined the ability of S. salivarius and S. mutans to form biofilms on selenium-free dental sealant discs, using the modified microtiter biofilm assay (Hammond et al, 2010;Tran et al, 2012). We recovered an average of 4 x 104 CFU/sealant disc for S. salivarius and an average of 2 x 105 CFU/sealant disc for S. mutans, using as control a selenium-free sealant (Figs.…”

“…The biofilm assay was performed, as previously described, by a modified microtiter plate assay (Hammond et al, 2010;Tran et al, 2012). Seven-millimeter discs of selenium-free dental sealant or organo-selenium dental sealants were incubated in 1 mL BHI media in the presence of approximately 1,000 initial colony-forming units (CFU) of S. salivarius or 7,500 CFU of S. mutans in each well of the microtiter plate.…”

“…The superoxide radical appears to account for most of selenium's toxicity toward different bacteria, such as Staphylococcus epidermidis, Staphylococcus aureus, Listeria monocytogenes, Salmonella typhimurium, and Escherichia coli in vitro (Babior et al, 1975;Bortolussi et al, 1987;Kramer and Ames, 1988). We previously showed that organo-selenium covalently attached to different biomaterials and medical devices, such as intravenous catheter, contact lenses, and reverse osmosis (RO) membranes, as well as cellulose, blocks the formation of Staphylococcus aureus and Pseudomonas aeruginosa biofilms (Mathews et al, 2006;Tran et al, 2009Tran et al, , 2012Reid et al, 2010;Low et al, 2011). Low concentrations of organo-selenium (0.1% or 0.2%) were sufficient to inhibit bacterial attachment to these materials.…”

Nanoscale Plasma Coating Inhibits Formation of Staphylococcus aureus Biofilm

“…Because animal studies have not yet been reported for these approaches, the in vivo efficacy of such antibiofilm techniques remains unclear. One possible exception is the recent report by Tran et al (18). These investigators coated hemodialysis catheters with an organoselenium compound through a wet coating process consisting of multiple steps and taking 3 days to complete; the organose-lenium-coated catheters inhibited S. aureus biofilms both in vitro and in vivo.…”