An orthotopic non-small cell lung cancer model for image-guided small animal radiotherapy platforms

Iglesias, Venus Sosa; Hoof, Stefan J van; Vaniqui, Ana; Schyns, Lotte E J R; Lieuwes, Natasja G.; Yaromina, Ala; Spiegelberg, Linda; Groot, Arjan J.; Verhaegen, Frank; Theys, Jan; Dubois, Ludwig; Vooijs, Marc

doi:10.1259/bjr.20180476

Cited by 19 publications

(23 citation statements)

References 36 publications

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“…The extended capabilities offered by small animal irradiators continues to be leveraged by investigators in delivering advanced preclinical protocols with wide-ranging experimental objectives. Treatment plans are being developed by combining CBTC datasets with online or sequential functional and anatomical imaging, which have been demonstrated in orthotopic pancreatic, lung and brain tumour models [54,68,69]. Other recent examples of innovative applications in the field include evaluation of radiosensitising strategies, such as hyperthermia [70] and drug CBCT, cone beam computed tomography; BLT, bioluminescence tomography; BLI, bioluminescence imaging.…”

Section: Preclinical Radiotherapy Techniquesmentioning

confidence: 99%

Evolution of the Supermodel: Progress in Modelling Radiotherapy Response in Mice

Butterworth

2019

Clinical Oncology

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Mouse models are essential tools in cancer research that have been used to understand the genetic basis of tumorigenesis, cancer progression and to test the efficacies of anticancer treatments including radiotherapy. They have played a critical role in our understanding of radiotherapy response in tumours and normal tissues and continue to evolve to better recapitulate the underlying biology of humans. In addition, recent developments in small animal irradiators have significantly improved in vivo irradiation techniques, allowing previously unimaginable experimental approaches to be explored in the laboratory. The combination of contemporary mouse models with small animal irradiators represents a major step forward for the radiobiology field in being able to much more accurately replicate clinical exposure scenarios. As radiobiology studies become ever more sophisticated in reflecting developments in the clinic, it is increasingly important to understand the basis and potential limitations of extrapolating data from mice to humans. This review provides an overview of mouse models and small animal radiotherapy platforms currently being used as advanced radiobiological research tools towards improving the translational power of preclinical studies.

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Section: Preclinical Radiotherapy Techniquesmentioning

confidence: 99%

Evolution of the Supermodel: Progress in Modelling Radiotherapy Response in Mice

Butterworth

2019

Clinical Oncology

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“…A major challenge is to develop robust models for sequential evaluation of tumour and normal tissue response in situ accounting for the multifactorial interactions within the tumour-normal tissue microenvironment which are known to be important mediators of radiation response. 41 A number of orthotopic tumour models involving implantation of human NSCLC cells into immuno-deficient mice have recently been reported 42,43 and may provide further insight 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65…”

Section: Challenges For Small Animal Radiotherapymentioning

confidence: 99%

Preclinical models of radiation-induced lung damage: challenges and opportunities for small animal radiotherapy

Ghita

Dunne

Hanna

et al. 2019

BJR

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“…Orthotopically implanted gliomas in syngeneic, immunocompetent animals are thought to provide the most accurate representation of the biological features of cancer growth and metastasis in humans [7]. Modern techniques now permit closer replication of clinical practice when irradiating orthotopic models, and recently developed treatment planning systems allow for better protection of healthy tissue from the radiation dose [4,5,8,9].…”

Section: Introductionmentioning

confidence: 99%

Use of a Luciferase-Expressing Orthotopic Rat Brain Tumor Model to Optimize a Targeted Irradiation Strategy for Efficacy Testing with Temozolomide

Mowday

Lieuwes

Biemans

et al. 2020

Cancers

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Glioblastoma multiforme (GBM) is a common and aggressive malignant brain cancer with a mean survival time of approximately 15 months after initial diagnosis. Currently, the standard-of-care (SOC) treatment for this disease consists of radiotherapy (RT) with concomitant and adjuvant temozolomide (TMZ). We sought to develop an orthotopic preclinical model of GBM and to optimize a protocol for non-invasive monitoring of tumor growth, allowing for determination of the efficacy of SOC therapy using a targeted RT strategy combined with TMZ. A strong correlation (r = 0.80) was observed between contrast-enhanced (CE)-CT-based volume quantification and bioluminescent (BLI)-integrated image intensity when monitoring tumor growth, allowing for BLI imaging as a substitute for CE-CT. An optimized parallel-opposed single-angle RT beam plan delivered on average 96% of the expected RT dose (20, 30 or 60 Gy) to the tumor. Normal tissue on the ipsilateral and contralateral sides of the brain were spared 84% and 99% of the expected dose, respectively. An increase in median survival time was demonstrated for all SOC regimens compared to untreated controls (average 5.2 days, p < 0.05), but treatment was not curative, suggesting the need for novel treatment options to increase therapeutic efficacy.

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An orthotopic non-small cell lung cancer model for image-guided small animal radiotherapy platforms

Cited by 19 publications

References 36 publications

Evolution of the Supermodel: Progress in Modelling Radiotherapy Response in Mice

Evolution of the Supermodel: Progress in Modelling Radiotherapy Response in Mice

Preclinical models of radiation-induced lung damage: challenges and opportunities for small animal radiotherapy

Use of a Luciferase-Expressing Orthotopic Rat Brain Tumor Model to Optimize a Targeted Irradiation Strategy for Efficacy Testing with Temozolomide

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