An Outbreak of Febrile Gastroenteritis Associated with Delicatessen Meat Contaminated with<i>Listeria monocytogenes</i>

Frye, Douglas; Zweig, Rachael; Sturgeon, Joan; Tormey, Michael P.; Lecavalier, M. A.; Lee, Irene; Lawani, Leonard; Mascola, Laurene

doi:10.1086/342582

Cited by 112 publications

(54 citation statements)

References 28 publications

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“…monocytogenes accounted for the greatest number of food product recalls (9,31,39) and several recent outbreaks (10,11,23,31). Therefore, rapid and sensitive methods are required to detect this pathogen in food.…”

Section: Discussionmentioning

confidence: 99%

Detection of Low Levels of Listeria monocytogenes Cells by Using a Fiber-Optic Immunosensor

Geng¹,

Morgan

Bhunia³

2004

Appl Environ Microbiol

117

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Biosensor technology has a great potential to meet the need for sensitive and nearly real-time microbial detection from foods. An antibody-based fiber-optic biosensor to detect low levels of Listeria monocytogenes cells following an enrichment step was developed. The principle of the sensor is a sandwich immunoassay where a rabbit polyclonal antibody was first immobilized on polystyrene fiber waveguides through a biotin-streptavidin reaction to capture Listeria cells on the fiber. Capture of cells on the fibers was confirmed by scanning electron microscopy. A cyanine 5-labeled murine monoclonal antibody, C11E9, was used to generate a specific fluorescent signal, which was acquired by launching a 635-nm laser light from an Analyte 2000 and collected by a photodetector at 670 to 710 nm. This immunosensor was specific for L. monocytogenes and showed a significantly higher signal strength than for other Listeria species or other microorganisms, including Escherichia coli, Enterococcus faecalis, Salmonella enterica, Lactobacillus plantarum, Carnobacterium gallinarum, Hafnia alvei, Corynebacterium glutamicum, Enterobacter aerogenes, Pseudomonas aeruginosa, and Serratia marcescens, in pure or in mixed-culture setup. Fiber-optic results could be obtained within 2.5 h of sampling. The sensitivity threshold was about 4.3 ؋ 10 3 CFU/ml for a pure culture of L. monocytogenes grown at 37°C. When L. monocytogenes was mixed with lactic acid bacteria or grown at 10°C with 3.5% NaCl, the detection threshold was 4

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Section: Discussionmentioning

confidence: 99%

Detection of Low Levels of Listeria monocytogenes Cells by Using a Fiber-Optic Immunosensor

Geng¹,

Morgan

Bhunia³

2004

Appl Environ Microbiol

117

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“…For all others not exquisitely sensitive to listeriosis, exposure to L. monocytogenes is seldom fatal. Several outbreaks of L. monocytogenes-associated febrile gastroenteritis have been reported among healthy adults, but only at doses of 10 5 CFU or greater (1,4,8,17). Concern about listeriosis led the Center for Food Safety and Applied Nutrition of the U.S. Food and Drug Administration (FDA), the Food Safety Inspection Service of the U.S. Department of Agriculture (USDA), and the Centers for Disease Control and Prevention (CDC) to conduct a risk assessment for L. monocytogenes in ready-to-eat foods (7).…”

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confidence: 99%

Dose-Response Model for Listeria monocytogenes -Induced Stillbirths in Nonhuman Primates

et al. 2008

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A dose-response model using rhesus monkeys as a surrogate for pregnant women indicates that oral exposure to 10 7 CFU of Listeria monocytogenes results in about 50% stillbirths. Ten of 33 pregnant rhesus monkeys exposed orally to a single dose of 10 2 to 10 10 CFU of L. monocytogenes had stillbirths. A log-logistic model predicts a dose affecting 50% of animals at 10 7 CFU, comparable to an estimated 10 6 CFU based on an outbreak among pregnant women but much less than the extrapolated estimate (10 13 CFU) from the FDA-U.S. Department of Agriculture-CDC risk assessment using an exponential curve based on mouse data. Exposure and etiology of the disease are the same in humans and primates but not in mice. This information will aid in risk assessment, assist policy makers, and provide a model for mechanistic studies of L. monocytogenes-induced stillbirths.

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“…Rarely, outbreaks have been caused by non-4b serotypes. For example, a serotype-3a strain outbreak was linked to contaminated butter in Finland (22), and a serotype-1/2a outbreak of gastrointestinal listeriosis was linked to sliced turkey in the United States (11). Previous studies have also shown that lineage I strains are more common among human listeriosis cases and outbreaks than among animal cases, while lineage III strains are significantly more common among animal listeriosis cases than among human cases (17).…”

mentioning

confidence: 99%

Listeria monocytogenesIsolates from Foods and Humans Form Distinct but Overlapping Populations

Gray

Zadoks

Fortes

et al. 2004

Appl Environ Microbiol

239

229

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. Assignment of isolates to lineages and to the majority of L. monocytogenes subtypes was significantly associated with the isolate source (food or human), although most subtypes and lineages included both human and food isolates. Some subtypes were also significantly associated with isolation from specific food types. Tissue culture plaque assay characterization of the 42 human isolates from Maryland and California and of 91 representative food isolates revealed significantly higher average infectivity and cell-to-cell spread for the human isolates, further supporting the hypothesis that food and human isolates form distinct populations. Combined analysis of subtype and cytopathogenicity data showed that strains classified into specific ribotypes previously linked to multiple human listeriosis outbreaks, as well as those classified into lineage I, are more common among human cases and generate larger plaques than other subtypes, suggesting that these subtypes may represent particularly virulent clonal groups. These data will provide a framework for prediction of the public health risk associated with specific L. monocytogenes subtypes.

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An Outbreak of Febrile Gastroenteritis Associated with Delicatessen Meat Contaminated withListeria monocytogenes

Cited by 112 publications

References 28 publications

Detection of Low Levels of Listeria monocytogenes Cells by Using a Fiber-Optic Immunosensor

Detection of Low Levels of Listeria monocytogenes Cells by Using a Fiber-Optic Immunosensor

Dose-Response Model for Listeria monocytogenes -Induced Stillbirths in Nonhuman Primates

Listeria monocytogenesIsolates from Foods and Humans Form Distinct but Overlapping Populations

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