An outbreak of food poisoning due to egg yolk reaction-negative Staphylococcus aureus

Miwa, Norinaga; Kawamura, Asako; Masuda, Takashi; Akiyama, Masato

doi:10.1016/s0168-1605(00)00446-3

Cited by 38 publications

(30 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The confirmed presence of the same resistance genes in staphylococci isolated from humans and animals, as well as the possibility of transference of genetic determinants between different species of bacteria, suggest that Staphylococcus bacteria, particularly CNS strains, are a reservoir of genes of antibiotic resistance in the natural environment (Aarestrup et al 2000, Khan et al 2000. Many authors also emphasize the role of poultry and poultry products as a significant source of outbreaks of food poisoning caused by Staphylococcus bacteria (Miwa et al 2001, Przybylska 2001.…”

Section: S Epidermidis N=3mentioning

confidence: 99%

Characterization of bacteria of the genus Staphylococcus isolated from the eggs of Japanese quail (Coturnix coturnix japonica)

Pyzik¹,

Marek²

2012

Polish Journal of Veterinary Sciences

View full text Add to dashboard Cite

The study attempted to analyse and characterize bacteria of the genus Staphylococcus isolated from the surface and contents of quail eggs, taking into account their phenotypic properties, biochemical reactions, antibiotic sensitivity patterns, and PCR to test for the presence of the mecA gene, which is responsible for resistance to methicillin. The study included 45 strains of the genus Staphylococcus isolated from the whites, yolks and shells of table quail eggs. The results obtained indicate that a fairly high percentage of the retail quail eggs tested were contaminated with Staphylococcus bacteria. Among the species isolated (11 in total), the most frequently occurring strains were of Staphylococcus hominis (26.7%), followed by Staphylococcus aureus (15.6%), S. xylosus and S. lentus (13.3% each), while percentages of the other Staphylococcus species were under 10%. The Staphylococcus strains tested had highly differentiated biochemical and enzymatic properties. As many as 7 biotypes were distinguished among the 7 S. aureus isolates, 6 biotypes within the species S. xylosus (6 strains tested), 5 biotypes among the 6 strains of S. lentus, but only 4 biotypes among the 12 S. hominis strains. The antibiotic sensitivity testing showed 15.5% of the strains to be resistant to one or more of the therapeutic agents tested. Moreover, some isolates exhibited intermediate sensitivity to the drugs, particularly to gentamicin (24.4%), neomycin (31.1%), streptomycin (46.7%) and Linco-Spectin (48.9%).

show abstract

Section: S Epidermidis N=3mentioning

confidence: 99%

Characterization of bacteria of the genus Staphylococcus isolated from the eggs of Japanese quail (Coturnix coturnix japonica)

Pyzik¹,

Marek²

2012

Polish Journal of Veterinary Sciences

View full text Add to dashboard Cite

show abstract

“…Although six prototypic SEs (SEA to SEE and SEH) have been implicated in outbreaks of food poisoning, SEA is most frequently implicated (Atanassova et al, 2001;Wieneke, 1974;Miwa et al, 2001). Some available commercial enzyme-linked immunosorbent assay (ELISA) kits for SE (SEA to SEE) detection are simple, sensitive (0.15 ng/mL), and rapid (Park et al, 1994), but have some drawbacks.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Identification Methods for Staphylococcus aureus and Development of Modified Detection Methods for Staphylococcal Enterotoxin A

Shimamura

Murata

2009

FSTR

View full text Add to dashboard Cite

We evaluated whether the agar plate method using a Baird Parker + rabbit plasma fibrinogen (RPF) agar could be used in place of the combination of a latex agglutination test (PS-latex) and a tube coagulase test for identification of Staphylococcus aureus from other strains of staphylococci. The PS-latex and tube coagulase tests showed positive results for 96 (100%) and 87 (90.6%) S. aureus strains, respectively. However, the PS-latex test showed positive results for 32 non-S. aureus strains. On the other hand, the agar plate method was positive for all the strains of S. aureus and showed no false-positive or -negative results. Next, we developed a highly sensitive and selective enzyme-linked immunosorbent assay (ELISA) for the detection of staphylococcal enterotoxin A (SEA). The interference by protein A and an unknown protein were eliminated using proteolytic digestion. This improved method is useful for SEA detection.

show abstract

“…Although the presence of 18 types of SE (SEA-SEE, and SEG-SEU) has been reported, SEA is most frequently implicated in food poisoning (Atanassova et al, 2001;Ichikawa et al, 1996;Miwa et al, 2001;Wieneke, 1974). Staphylococcal food poisoning is often associated with food that is extensively manipulated by hand (Atanassova et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

“…In Japan, the coagulase-typing method has been used successfully in epidemiological investigations of staphylococcal food poisoning. It has been reported that coagulase type VII (accounting for about 70% of the total outbreaks), III, II, and VI are, respectively, the first, second, third, and fourth most predominant types of coagulase responsible for food poisoning incidents throughout Japan (Ichikawa et al, 1996;Miwa et al, 2001;Terayama and Igarashi, 1981;Wieneke et al, 1974). The contamination of Japanese foods by S. aureus and the enterotoxigenicity of isolates have been examined (Igarashi, 1993;Oda, 1998;Shimizu et al, 2000;Terayama, 1977).…”

Section: Introductionmentioning

confidence: 99%

Relationship among Properties of Staphylococcus aureus Isolated from Retail Foods and Human Hands, and Distribution of MRSA

Shimamura

Murata²

2008

FSTR

View full text Add to dashboard Cite

The relationship among staphylococcal enterotoxin (SE) type, coagulase type and production of β-lactamase of Staphylococcus aureus isolates was investigated. A total of 131 S. aureus strains isolated from food samples, human hands and environmental samples were used. The predominant type of SE was SEB (66.0%) and most strains producing SEB belonged to coagulase type VI (51.4%). β-Lactamase was detected in 37 (60.3%) of the 53 SE-positive isolates. Seven (88.9%) of the 8 SEA-producing isolates produced β-lactamase. One isolate from a healthy human hand was mecA-positive methicillin-resistant S. aureus, which showed coagulase type VIII and did not produce SE. Although PCR analysis was performed to determine the staphylococcal cassette chromosome mec (SCCmec) type of this strain, SCCmec type was not classified and this strain did not carry the Panton-Valentine leukocidin (PVL) gene.

show abstract

An outbreak of food poisoning due to egg yolk reaction-negative Staphylococcus aureus

Cited by 38 publications

References 11 publications

Characterization of bacteria of the genus Staphylococcus isolated from the eggs of Japanese quail (Coturnix coturnix japonica)

Characterization of bacteria of the genus Staphylococcus isolated from the eggs of Japanese quail (Coturnix coturnix japonica)

Evaluation of Identification Methods for Staphylococcus aureus and Development of Modified Detection Methods for Staphylococcal Enterotoxin A

Relationship among Properties of Staphylococcus aureus Isolated from Retail Foods and Human Hands, and Distribution of MRSA

Contact Info

Product

Resources

About