An Outbreak of Human Coronavirus OC43 during the 2014&amp;ndash;2015 Influenza Season in Yamagata, Japan

Matoba, Yohei; Aoki, Yoko; Tanaka, Shizuka; Yahagi, Kazue; Shimotai, Yoshitaka; Matsuzaki, Yoko; Itagaki, Tsutomu; Mizuta, Katsumi

doi:10.7883/yoken.jjid.2015.292

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Isolation Of Clinical Isolates Of Hcovs By Ali Culture1

Communicated By Makoto Takeda1

-5 Human Coronaviruses1

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Cited by 12 publications

(21 citation statements)

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“…In temperate climates, HCoVs infections occur predominantly in the winter and early spring (1). Our longitudinal observation of HCoVs in Yamagata, Japan supported the seasonality of these outbreaks (2,3). However, we surprisingly experienced an outbreak of HCoV-OC43 in early summer in…”

supporting

confidence: 71%

Trends of Human Coronaviruses in Yamagata, Japan in 2015–2016 Focusing on the OC43 Outbreak of June 2016

et al. 2018

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supporting

confidence: 71%

Trends of Human Coronaviruses in Yamagata, Japan in 2015–2016 Focusing on the OC43 Outbreak of June 2016

et al. 2018

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“…Specimens diagnosed as containing HCoVs by real-time RT-PCR were used for virus isolation. Recently, the circulating HCoV strain in Japan has been biased toward HCoV-OC43 (Hara and Takao, 2015;Matoba et al, 2015;Yano et al, 2014). Therefore, we did not obtain specimens positive for HCoV-229E or HCoV-NL63; however, four HCoV-OC43 and two HCoV-HKU1 isolates were finally isolated successfully (Table 1).…”

Section: Isolation Of Clinical Isolates Of Hcovs By Ali Culturementioning

confidence: 99%

Wild-type human coronaviruses prefer cell-surface TMPRSS2 to endosomal cathepsins for cell entry

2018

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Human coronaviruses (HCoVs) enter cells via two distinct pathways: the endosomal pathway using cathepsins to activate spike protein and the cell-surface or early endosome pathway using extracellular proteases such as transmembrane protease serine 2 (TMPRSS2). We previously reported that clinical isolates of HCoV-229E preferred cell-surface TMPRSS2 to endosomal cathepsin for cell entry, and that they acquired the ability to use cathepsin L by repeated passage in cultured cells and were then able to enter cells via the endosomal pathway. Here, we show that clinical isolates of HCoV-OC43 and -HKU1 preferred the cell-surface TMRRSS2 to endosomal cathepsins for cell entry, similar to HCoV-229E. In addition, the cell-culture-adapted HCoV-OC43 lost the ability to infect and replicate in air-liquid interface cultures of human bronchial tracheal epithelial cells. These results suggest that circulating HCoVs in the field generally use cell-surface TMPRSS2 for cell entry, not endosomal cathepsins, in human airway epithelial cells.

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“…We carried out pilot molecular screening studies for human coronaviruses (HCoVs) using nasopharyngeal samples from children with ARIs. These studies revealed that 4 HCoVs (HCoV-229E, -OC43, -NL63, and -HKU1) were in circulation in Yamagata between 2010 and 2015 (2,3).…”

Section: Communicated By Makoto Takedamentioning

confidence: 99%

HeLa-ACE2-TMPRSS2 Cells Are Useful for the Isolation of Human Coronavirus 229E

et al. 2016

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An Outbreak of Human Coronavirus OC43 during the 2014–2015 Influenza Season in Yamagata, Japan

Cited by 12 publications

References 10 publications

Trends of Human Coronaviruses in Yamagata, Japan in 2015–2016 Focusing on the OC43 Outbreak of June 2016

Trends of Human Coronaviruses in Yamagata, Japan in 2015–2016 Focusing on the OC43 Outbreak of June 2016

Wild-type human coronaviruses prefer cell-surface TMPRSS2 to endosomal cathepsins for cell entry

HeLa-ACE2-TMPRSS2 Cells Are Useful for the Isolation of Human Coronavirus 229E

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