An outbreak of sheep pox associated with goat poxvirus in Gansu province of China

Yan, Xingcheng; Chu, Yuefeng; Wu, Guohua; Zhao, Zhongwei; Li, Jian; Zhu, Hongwei; Zhang, Qiang

doi:10.1016/j.vetmic.2011.11.015

Cited by 49 publications

(37 citation statements)

References 10 publications

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“…After the viruses underwent 6 passages in LT cells, CPEs were prominent (48 h post-infection). The viruses were harvested 5-6 days post-infection (Yan et al, 2012). (Figure 2A).…”

Section: Resultsmentioning

confidence: 99%

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Identification and phylogenetic analysis of a sheep pox virus isolated from the Ningxia Hui Autonomous Region of China

Zhu¹,

Yang²,

Li³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. An outbreak of sheep pox was investigated in the Ningxia Hui Autonomous Region in China. Through immunofluorescence testing, isolated viruses, polymerase chain reaction identification, and electron microscopic examination, the isolated strain was identified as a sheep pox virus. The virus was identified through sequence and phylogenetic analysis of the P32 gene, open reading frame (ORF) 095, and ORF 103 genes. This study is the first to use the ORF 095 and ORF 103 genes as candidate genes for the analysis of sheep pox. The results showed that the ORF 095 and ORF 103 genes could be used for the genotyping of the sheep pox virus.

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“…After the viruses underwent 6 passages in LT cells, CPEs were prominent (48 h post-infection). The viruses were harvested 5-6 days post-infection (Yan et al, 2012). (Figure 2A).…”

Section: Resultsmentioning

confidence: 99%

“…The most frequently used technique is still PCR, and the P32 gene has been developed for the differentiation of CaPVs (Hosamani et al, 2004). The G protein-coupled receptor gene (Le Goff et al, 2009) and the 30-kDa RNA polymerase subunit gene (Lamien et al, 2011) are also being considered for use in PCR testing of CaPVs (Yan et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Identification and phylogenetic analysis of a sheep pox virus isolated from the Ningxia Hui Autonomous Region of China

Zhu¹,

Yang²,

Li³

et al. 2013

Genet. Mol. Res.

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“…Characterization of these viruses needs molecular techniques targeting CaPVs specific genes like P32, GPCR and RPO30 genes (Zhou et al, 2010;Yan et al, 2012). Unfortunately, the knowledge on the current situation of Capripoxvirus infection of sheep and goats in Saudi Arabia is very scanty.…”

Section: Introductionmentioning

confidence: 99%

Molecular detection and phylogenetic analysis of sheeppox virus in Al - Hassa of Eastern Province of Saudi Arabia

Al-Shabebi¹,

El-Sabagh²,

Abuelzein³

et al. 2014

Adv. Anim. Vet. Sci.

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The current study was conducted to molecularly identify Sheep pox virus (SPPV) from recent outbreak affected sheep farms in Al-Hassa of Eastern Province of Saudi Arabia during the period between the winter and the spring, 2013. Thirty skin lesions of crusted scab samples were collected from six clinically infected SPPV sheep showing average morbidity and mortality rates of 80% and 15%, respectively. The collected samples were screened for presence of SPPV DNAs using KS-1.5/KS-1.6 and InS-1.1/InS-1.1 / -based multiplex PCR. The P32 gene of selected two positive samples was sequenced and aligned with different SPPV, GTPV and LSDV available in GenBank. The phylogenetic analysis revealed that the Sheep pox virus strain; SPPV/Al-Hassa/2013/Saudi Arabia (accession number, KF204447) clustered on SPPV clade with SPPVs from India and China. This finding elucidated that the causative agent of this recent sheep pox outbreak is SPPV.All copyrights reserved to Nexus® academic publishers

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“…[26] and Yan et al . [29]. In the past, detection and identification of CaPVs were mentioned in several PCR assays [4,14-17].…”

Section: Discussionmentioning

confidence: 99%

Detection, identification, and differentiation of sheep pox virus and goat pox virus from clinical cases in Giza Governorate, Egypt

Mahmoud

Khafagi

2016

Vet World

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Aim:To isolate, identify, and differentiate Capripoxviruses (CaPV) (sheep pox virus and goat pox virus) infections by egg inoculation, transmission electron microscopy (TEM), and 30 kDa RNA polymerase subunit gene-based polymerase chain reaction (PCR) (RPO30) in clinically affected animals in Hawamdia township of Giza Governorate, Egypt.Materials and Methods:A total of 37 scab samples were collected from clinically suspected field cases of sheep pox and goat pox. These samples were collected during (2014-2015) during different outbreaks of sheep pox and goat pox from Hawamdia township of Giza Governorate, Egypt. The samples were subjected to egg inoculation, TEM, and (RPO30) gene-based PCR. By using the egg inoculation: Previously prepared 37 scab samples (n=23 sheep and n=14 goats) were inoculated on the chorioallantoic membrane of specific pathogen free (SPF) embryonated chicken eggs (12 days old age). In the presence of the suitable percentage of humidity and candling, the inoculated eggs were incubated at 37°C. By using the TEM: Samples showed positive pock lesions on the chorioallantoic membranes, were fixed in glutaraldehyde, then processed and sectioned for TEM. Using the (RPO30) gene-based PCR assay, 30 of positive samples after egg inoculation (n=19 sheep and n=11 goats) were screened.Results:Using the egg inoculation, a characteristic pock lesions for poxviruses were seen in 30/37 (n=19 sheep and n=11 goats) (81.08%). Using the TEM, examination of the positive samples after egg inoculation revealed positive result in 23/30 (n=15 sheep and n=8 goats) (76.66%). The positive results represented by the presence of negatively stained oval-shape virus particles. Using the (RPO30) gene-based PCR assay, out of 30 total of positive samples after egg inoculation (n=19 sheep and n=11 goats) were screened, 27 (90%) samples (n=17 sheep and n=10 goats) were positive. The given band sizes of sheep and goats were 172 and 152 bp, respectively.Conclusion:PCR assay depended on RPO30 gene can be used lonely for the detection, identification, and differentiation of CaPVs. RPO30 gene-based PCR assay in combination with gene sequencing helps in molecular epidemiological studies of CaPV infection.

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An outbreak of sheep pox associated with goat poxvirus in Gansu province of China

Cited by 49 publications

References 10 publications

Identification and phylogenetic analysis of a sheep pox virus isolated from the Ningxia Hui Autonomous Region of China

Identification and phylogenetic analysis of a sheep pox virus isolated from the Ningxia Hui Autonomous Region of China

Molecular detection and phylogenetic analysis of sheeppox virus in Al - Hassa of Eastern Province of Saudi Arabia

Detection, identification, and differentiation of sheep pox virus and goat pox virus from clinical cases in Giza Governorate, Egypt

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