An outer membrane determinant for RNA phage genome entry in Pseudomonas aeruginosa

Bae, Hee-Won; Choi, Shin-Yae; Cho, You-Hee

doi:10.1016/j.isci.2023.108675

iScience

2024

DOI: 10.1016/j.isci.2023.108675

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An outer membrane determinant for RNA phage genome entry in Pseudomonas aeruginosa

Hee-Won Bae,

Shin-Yae Choi,

You-Hee Cho

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2024

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A bacterial site 2 protease contributes to RNA phage resistance by targeting phage lysis protein

Bae,

Kim,

Choi

et al. 2024

Preprint

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Lytic phages culminate their lifecycle by causing lysis of the infected host cell. Despite extensive research on the molecular mechanisms of phage lysis, our understanding of anti-phage resistance mechanisms during the lysis stage remains less understood. Here, we demonstrated that MucP, a site 2 protease of Pseudomonas aeruginosa (PA), mitigates the activity of the RNA phage PP7 lysis protein (LP), which contains a transmembrane (TM) helix, suggesting that MucP act as a resistance mechanism against PP7-induced lysis. We identified an LP variant (LP*) having enhanced helical propensity due to P26L and S40L mutations, which was unaffected by MucP and exhibited killing activity against PA strains that are resistant to the wild type LP, with an inverse correlation between MucP activity and LP susceptibility. A PP7 mutant with LP* exhibited MucP-escaper phenotype such as discernable plaque formation on MucP-expressing cells. These results suggest that MucP targets the RNA phage LP at the TM helix in certain strains, providing a resistance function compromising phage lysis by utilizing an existing bacterial enzyme in PA.

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A bacterial site 2 protease contributes to RNA phage resistance by targeting phage lysis protein

Bae,

Kim,

Choi

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

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An outer membrane determinant for RNA phage genome entry in Pseudomonas aeruginosa

Cited by 1 publication

References 43 publications

A bacterial site 2 protease contributes to RNA phage resistance by targeting phage lysis protein

A bacterial site 2 protease contributes to RNA phage resistance by targeting phage lysis protein

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