An Overview Focusing on Food Liposomes and Their Stability to Electric Fields

Jara-Quijada, Erick; Pérez‐Won, Mario; Tabilo‐Munizaga, Gipsy; González-Cavieres, Luis; Lemus‐Mondaca, Roberto

doi:10.1007/s12393-022-09306-2

Cited by 5 publications

(4 citation statements)

References 143 publications

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“…Katouzian and Taheri ( 2021 ) reported an increase in electrical conductivity when incorporating chitosan in chitosan-coated liposomes, which is related to the results obtained in this study. Jara-Quijada et al ( 2022 ) mentioned that the electrical conductivity of liposomes mainly depends on their membrane composition, biological nature of phospholipids, phospholipid polarity, asymmetric membranes, cholesterol content, and surfactant content.…”

Section: Resultsmentioning

confidence: 99%

“…From an operational point of view, one of the most critical challenges in applying liposomes in the food industry is their relative instability in aqueous dispersions (Nakhaei et al, 2021 ); this can lead to unwanted effects such as reduced encapsulation efficiency (EE%) (Toh & Chiu, 2013 ), oxidation, hydrolysis (Frenzel & Steffen-Heins, 2015 ), membrane binding, and aggregation (Rahdar et al, 2019 ). Recently, Jara-Quijada et al ( 2022 ) reported that the modification of the membrane surface is an important factor in the design of liposomes. Cholesterol has been shown to provide stability to liposomes (Nakhaei et al, 2021 ), such as regulation of permeability, elasticity, rigidity, membrane strength, matter transport (Ermilova & Lyubartsev, 2019 ), thermal stability (Jovanović et al, 2018 ), and electrical (Kotnik et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

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Liposomes Loaded with Green Tea Polyphenols—Optimization, Characterization, and Release Kinetics Under Conventional Heating and Pulsed Electric Fields

Jara-Quijada

Pérez‐Won

Tabilo‐Munizaga

et al. 2023

Food Bioprocess Technol

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This study aimed to increase the encapsulation efficiency (EE%) of liposomes loaded with green tea polyphenols (GTP), by optimizing with response surface methodology (RSM), characterizing the obtained particles, and modeling their release under conventional heating and pulsed electric fields. GTP-loaded liposomes were prepared under conditions of Lecithin/Tween 80 (4:1, 1:1, and 1:4), cholesterol (0, 30, and 50%), and chitosan as coating (0, 0.05, and 0.1%). Particles were characterized by size, polydispersity index, ζ -potential, electrical conductivity, and optical microscopy. The release kinetics was modeled at a temperature of 60 °C and an electric field of 5.88 kV/cm. The optimal manufacturing conditions of GTP liposomes (ratio of lecithin/Tween 80 of 1:1, cholesterol 50%, and chitosan 0.1%) showed an EE% of 60.89% with a particle diameter of 513.75 nm, polydispersity index of 0.21, ζ -potential of 33.67 mV, and electrical conductivity of 0.14 mS/cm. Optical microscopy verified layering in the liposomes. The kinetic study revealed that the samples with chitosan were more stable to conventional heating, and those with higher cholesterol content were more stable to pulsed electric fields. However, in both treatments, the model with the best fit was the Peppas model. The results of the study allow us to give an indication of the knowledge of the behavior of liposomes under conditions of thermal and non-thermal treatments, helping the development of new functional ingredients based on liposomes for processed foods.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Liposomes Loaded with Green Tea Polyphenols—Optimization, Characterization, and Release Kinetics Under Conventional Heating and Pulsed Electric Fields

Jara-Quijada

Pérez‐Won

Tabilo‐Munizaga

et al. 2023

Food Bioprocess Technol

Self Cite

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“…Manufacturers should effectively communicate the benefits and safety of liposome-enriched foods by emphasizing nutrient delivery, safety, controlled release, masking flavors, targeted delivery, longer shelf life, and transparent education. By doing so, they can build consumer trust and encourage the adoption of these innovative food products [ 108 , 121 ].…”

Section: Consumer Expectationsmentioning

confidence: 99%

Liposomes as Carriers of Bioactive Compounds in Human Nutrition

Rudzińska,

Grygier,

Knight

et al. 2024

Foods

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This article provides an overview of the literature data on the role of liposomal structures and encapsulated substances in food technology and human nutrition. The paper briefly describes how liposomes are created and how they encapsulate food ingredients, which can either be individual compounds or plant extracts. Another very interesting application of liposomes is their use as antimicrobial carriers to protect food products from spoilage during storage. The encapsulation of food ingredients in liposomes can increase their bioavailability, which is particularly important for compounds with health-promoting properties but low bioavailability. Particular attention was paid to compounds such as phytosterols, which lower blood cholesterol levels but have very low absorption in the human body. In addition, consumer expectations and regulations for liposomes in food are discussed. To date, no in vivo human studies have been conducted to indicate which encapsulation methods give the best results for gastrointestinal effects and which food-added substances are most stable during food storage and processing. The paper identifies further lines of research that are needed before liposomes can be introduced into food.

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“…Conversely, liposomes having positive charge are readily taken up by the cells due to electrostatic attraction between liposomes and negatively charged cell membrane [ 165 ]. In terms of stability and pores formation under electric field stimulation, negatively charged liposomes (i.e., POPS-1-palmitoyl-2-oleoyl-sn-glycerol-3-phospho- L-serine) allow greater ion transport by forming large hydrophobic pores compare to positively charged (i.e., POPC-1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphatidylcholine) [ 166 , 167 ].…”

Section: Electroporationmentioning

confidence: 99%

Electroporation in Head-and-Neck Cancer: An Innovative Approach with Immunotherapy and Nanotechnology Combination

Pisani

Bertino

Prina-Mello

et al. 2022

Cancers

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Squamous cell carcinoma is the most common malignancy that arises in the head-and-neck district. Traditional treatment could be insufficient in case of recurrent and/or metastatic cancers; for this reason, more selective and enhanced treatments are in evaluation in preclinical and clinical trials to increase in situ concentration of chemotherapy drugs promoting a selectively antineoplastic activity. Among all cancer treatment types (i.e., surgery, chemotherapy, radiotherapy), electroporation (EP) has emerged as a safe, less invasive, and effective approach for cancer treatment. Reversible EP, using an intensive electric stimulus (i.e., 1000 V/cm) applied for a short time (i.e., 100 μs), determines a localized electric field that temporarily permealizes the tumor cell membranes while maintaining high cell viability, promoting cytoplasm cell uptake of antineoplastic agents such as bleomycin and cisplatin (electrochemotherapy), calcium (Ca2+ electroporation), siRNA and plasmid DNA (gene electroporation). The higher intracellular concentration of antineoplastic agents enhances the antineoplastic activity and promotes controlled tumor cell death (apoptosis). As secondary effects, localized EP (i) reduces the capillary blood flow in tumor tissue (“vascular lock”), lowering drug washout, and (ii) stimulates the immune system acting against cancer cells. After years of preclinical development, electrochemotherapy (ECT), in combination with bleomycin or cisplatin, is currently one of the most effective treatments used for cutaneous metastases and primary skin and mucosal cancers that are not amenable to surgery. To reach this clinical evidence, in vitro and in vivo models were preclinically developed for evaluating the efficacy and safety of ECT on different tumor cell lines and animal models to optimize dose and administration routes of drugs, duration, and intensity of the electric field. Improvements in reversible EP efficacy are under evaluation for HNSCC treatment, where the focus is on the development of a combination treatment between EP-enhanced nanotechnology and immunotherapy strategies.

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An Overview Focusing on Food Liposomes and Their Stability to Electric Fields

Cited by 5 publications

References 143 publications

Liposomes Loaded with Green Tea Polyphenols—Optimization, Characterization, and Release Kinetics Under Conventional Heating and Pulsed Electric Fields

Liposomes Loaded with Green Tea Polyphenols—Optimization, Characterization, and Release Kinetics Under Conventional Heating and Pulsed Electric Fields

Liposomes as Carriers of Bioactive Compounds in Human Nutrition

Electroporation in Head-and-Neck Cancer: An Innovative Approach with Immunotherapy and Nanotechnology Combination

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