An Overview of Analytical Methodologies for Determination of Vancomycin in Human Plasma

Abstract

Vancomycin is regarded as the last resort of defense for a wide range of infections due to drug resistance and toxicity. The detection of vancomycin in plasma has always aroused particular concern because the performance of the assay affects the clinical treatment outcome. This article reviews various methods for vancomycin detection in human plasma and analyzes the advantages and disadvantages of each technique. Immunoassay has been the first choice for vancomycin concentration monitoring due to its simplicity and practicality, occasionally interfered with by other substances. Chromatographic methods have mainly been used for scientific research due to operational complexity and the particular requirement of the instrument. However, the advantages of a small amount of sample needed, high sensitivity, and specificity makes chromatography irreplaceable. Other methods are less commonly used in clinical applications because of the operational feasibility, clinical application, contamination, etc. Simplicity, good performance, economy, and environmental friendliness have been points of laboratory methodological concern. Unfortunately, no one method has met all of the elements so far.

Fluorescence polarization immunoassay for the determination and screening of medicines.

“…Однако использование метода FPIA в клинике было ограничено и другими проблемами -это неспецифическое связывание флуоресцентно меченного антигена с белками сыворотки и ее собственная флуоресценция. Для ряда препаратов эти проблемы были решены, и разработаны тесты для определения различных терапевтических препаратов в плазме или сыворотке крови, включая гентамицин, карбамазепин [32,33], теофиллин [34], фенитоин и фенобарбитал [35], ванкомицин [36], и было показано,…”

Analisis Senyawa Obat Vankomisin dalam Plasma Darah dengan Berbagai Metode

“…Analisis vankomisin dalam plasma darah telah banyak dilakukan dengan menggunakan berbagai metode seperti bioassay, radioimmunoassay (RIA), fluorescence polarization immunoassay (FPIA), metode enzyme-multiplied immunoassay (EMIT), kromatografi cair kinerja tinggi (KCKT), kromatografi cair-spektrometri massa (LC-MS/MS), dan metode lainnya (Cheng, et al, 2022). Karena selektivitas, sensitivitas, dan kemudahan penggunaan, kromatografi cairspektrometri massa (LC-MS/MS) adalah pilihan analisis yang sesuai (Zhang et al, 2019).…”

Diagnostic value of BHI-V4 for heterogeneous and vancomycin-intermediate Staphylococcus aureus isolates: a systematic review and meta-analysis

“…This bacterium has developed resistance to multiple antibiotics, making it difficult to treat and increasing the risk of severe and prolonged infections. Initially, vancomycin was considered the last resort for treating multidrug-resistant Staphylococcus aureus (MRSA) [ 2 ]. However, the subsequent discovery of heterogeneous (hVISA) and vancomycin-intermediate Staphylococcus aureus (VISA) has posed new challenges in the clinical treatment of these pathogens [ 3 ].…”