An overview of chromatographic methods coupled with mass spectrometric detection for determination of angiotensin-converting enzyme inhibitors in biological material

Validated Chromatographic Methods for Simultaneous Determination of Amlodipine Besylate and Perindopril Arginine in Binary Mixtures and in Pharmaceutical Dosage Form

“…On the other hand decreasing acetonitrile ratio in the mobile phase (less than 40%) did not affect the resolution but decrease the analysis time. Also phosphate buffer with different pH values was tested (3)(4)(5)(6) where the optimum separation with symmetric untailed peaks was obtained when using pH=4.6. The effect of the mobile phase flow rate (1, 1.5 and 2 mL.min -1 ) on the separation was also tested, where using flow rate of 1 mL.min -1 gave the optimum chromatographic separation within reasonable time analysis.…”

“…The literature survey revealed that there are some analytical methods for the determination of Perindopril Erbumine including Potentiometric [1], HPLC [5], LC-MS [6] and CE [7]. Amlodipine besylate was determined by spectrophotometric [8][9][10], Spectrofluorometric [11], LC [1], RP-HPLC [12][13][14], LC-MS [15], HPTLC [16] and HPLC stability indicating assay method [17].…”

Development of On-Line High Performance Liquid Chromatography (HPLC)-Biochemical Detection Methods as Tools in the Identification of Bioactives

“…Rudzki et al [64] reviewed the use of techniques hyphenating HPLC and gas chromatography with MS to search for ACE inhibitors based on chemical structure. By combining information from a myriad of publications they emphasized the importance of stereochemical purity of synthesized inhibitors since ( S )-stereo-isomers were active while ( R )-stereo-isomers were not.…”

Captopril Determination in Dried Blood Spot Samples with LC-MS and LCHRMS: A Potential Method for Neonate Pharmacokinetic Studies

“…To determine captopril or its metabolites in blood or plasma, several analytical methods have been described including HPLC with UV and fluorescence detection [6][7][8][9][10], liquid chromatographymass spectrometry (LC-MS), liquid chromatography tandem mass spectrometry (LC-MS/MS) [11][12][13][14], gas chromatography-mass spectrometry (GC-MS) [15,16], radioimmunoassay (RIA) [6] and enzyme immunoassay [6] with the HPLC methods most widely investigated. Although the analytical literature seems abundant for the determination of captopril in human blood or plasma, a method using standard analytical equipment providing a sensitive and simple micro-analytical method for determining captopril from a small blood volume has not been addressed and was therefore investigated in the present study.…”